Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_6016 and Dshi_6017 are separated by 196 nucleotides Dshi_6017 and Dshi_0625 are separated by 109 nucleotides Dshi_0625 and Dshi_0626 are separated by 230 nucleotides Dshi_0626 and Dshi_0627 overlap by 8 nucleotides
Dshi_6016: Dshi_6016 - 23SrRNA (RefSeq), at 619,764 to 622,680
_6016
Dshi_6017: Dshi_6017 - 5SrRNA (RefSeq), at 622,877 to 622,994
_6017
Dshi_0625: Dshi_0625 - hypothetical protein (RefSeq), at 623,104 to 623,826
_0625
Dshi_0626: Dshi_0626 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 624,057 to 624,743
_0626
Dshi_0627: Dshi_0627 - ABC transporter related (RefSeq), at 624,736 to 625,491
_0627
Position (kb) 623
624 Strain fitness (log2 ratio) -2
-1
0
1 at 623.220 kb on - strand, within Dshi_0625 at 623.481 kb on - strand, within Dshi_0625 at 624.259 kb on - strand, within Dshi_0626 at 624.305 kb on + strand, within Dshi_0626 at 624.313 kb on + strand, within Dshi_0626 at 624.391 kb on + strand, within Dshi_0626 at 624.397 kb on - strand, within Dshi_0626 at 624.444 kb on + strand, within Dshi_0626 at 624.501 kb on - strand, within Dshi_0626 at 624.522 kb on - strand, within Dshi_0626 at 624.563 kb on - strand, within Dshi_0626 at 624.679 kb on + strand at 624.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 623,220 - Dshi_0625 0.16 -2.2 623,481 - Dshi_0625 0.52 -0.7 624,259 - Dshi_0626 0.29 +0.1 624,305 + Dshi_0626 0.36 -1.0 624,313 + Dshi_0626 0.37 +1.6 624,391 + Dshi_0626 0.49 -0.7 624,397 - Dshi_0626 0.49 +0.5 624,444 + Dshi_0626 0.56 -0.0 624,501 - Dshi_0626 0.65 -0.0 624,522 - Dshi_0626 0.68 -0.3 624,563 - Dshi_0626 0.74 -0.4 624,679 + -0.1 624,761 + -0.3
Or see this region's nucleotide sequence