Experiment: PYE 0.5x 25C survival rep3 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02008 and CCNA_02009 overlap by 1 nucleotides CCNA_02009 and CCNA_02010 are separated by 18 nucleotides CCNA_02010 and CCNA_02011 overlap by 1 nucleotides CCNA_02011 and CCNA_02012 are separated by 108 nucleotides
CCNA_02008: CCNA_02008 - prolyl-tRNA synthetase, at 2,155,544 to 2,156,875
_02008
CCNA_02009: CCNA_02009 - hypothetical protein, at 2,156,875 to 2,157,291
_02009
CCNA_02010: CCNA_02010 - hypothetical protein, at 2,157,310 to 2,157,570
_02010
CCNA_02011: CCNA_02011 - methylmalonyl CoA epimerase, at 2,157,570 to 2,157,989
_02011
CCNA_02012: CCNA_02012 - metal-dependent hydrolase, at 2,158,098 to 2,159,777
_02012
Position (kb)
2157
2158 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2156.914 kb on - strand at 2156.925 kb on - strand, within CCNA_02009 at 2156.958 kb on + strand, within CCNA_02009 at 2156.985 kb on + strand, within CCNA_02009 at 2157.031 kb on + strand, within CCNA_02009 at 2157.047 kb on - strand, within CCNA_02009 at 2157.064 kb on + strand, within CCNA_02009 at 2157.071 kb on - strand at 2157.072 kb on - strand, within CCNA_02009 at 2157.072 kb on - strand, within CCNA_02009 at 2157.231 kb on + strand, within CCNA_02009 at 2157.231 kb on + strand, within CCNA_02009 at 2157.239 kb on - strand, within CCNA_02009 at 2157.277 kb on - strand at 2157.295 kb on - strand at 2157.327 kb on - strand at 2157.335 kb on + strand at 2157.335 kb on - strand at 2157.544 kb on - strand, within CCNA_02010 at 2157.557 kb on + strand at 2157.568 kb on + strand at 2157.576 kb on - strand at 2157.576 kb on - strand at 2157.576 kb on - strand at 2157.626 kb on + strand, within CCNA_02011 at 2157.648 kb on - strand, within CCNA_02011 at 2157.696 kb on - strand, within CCNA_02011 at 2157.696 kb on - strand, within CCNA_02011 at 2157.699 kb on + strand, within CCNA_02011 at 2157.729 kb on - strand, within CCNA_02011 at 2157.729 kb on - strand, within CCNA_02011 at 2157.735 kb on - strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.771 kb on - strand, within CCNA_02011 at 2157.771 kb on - strand, within CCNA_02011 at 2157.816 kb on - strand, within CCNA_02011 at 2157.866 kb on - strand, within CCNA_02011 at 2157.868 kb on - strand, within CCNA_02011 at 2157.910 kb on - strand, within CCNA_02011 at 2157.920 kb on - strand, within CCNA_02011 at 2157.978 kb on - strand at 2157.996 kb on - strand at 2157.996 kb on - strand at 2158.030 kb on + strand at 2158.036 kb on + strand at 2158.066 kb on - strand at 2158.087 kb on + strand at 2158.109 kb on + strand at 2158.137 kb on - strand at 2158.176 kb on - strand at 2158.176 kb on - strand at 2158.260 kb on + strand at 2158.268 kb on - strand, within CCNA_02012 at 2158.268 kb on - strand, within CCNA_02012 at 2158.268 kb on - strand, within CCNA_02012 at 2158.275 kb on - strand, within CCNA_02012 at 2158.295 kb on - strand, within CCNA_02012 at 2158.317 kb on - strand, within CCNA_02012 at 2158.445 kb on + strand, within CCNA_02012 at 2158.453 kb on - strand, within CCNA_02012 at 2158.567 kb on + strand, within CCNA_02012
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 25C survival rep3 12 days remove 2,156,914 - -0.2 2,156,925 - CCNA_02009 0.12 +0.0 2,156,958 + CCNA_02009 0.20 +0.3 2,156,985 + CCNA_02009 0.26 -1.2 2,157,031 + CCNA_02009 0.37 -0.7 2,157,047 - CCNA_02009 0.41 -0.4 2,157,064 + CCNA_02009 0.45 -1.2 2,157,071 - -0.5 2,157,072 - CCNA_02009 0.47 -1.8 2,157,072 - CCNA_02009 0.47 -0.7 2,157,231 + CCNA_02009 0.85 -1.7 2,157,231 + CCNA_02009 0.85 +1.3 2,157,239 - CCNA_02009 0.87 -0.7 2,157,277 - +0.4 2,157,295 - +0.1 2,157,327 - +0.1 2,157,335 + -0.8 2,157,335 - +0.3 2,157,544 - CCNA_02010 0.90 -5.3 2,157,557 + +0.0 2,157,568 + +2.3 2,157,576 - +0.5 2,157,576 - +0.4 2,157,576 - +0.2 2,157,626 + CCNA_02011 0.13 +0.3 2,157,648 - CCNA_02011 0.19 -3.3 2,157,696 - CCNA_02011 0.30 -4.7 2,157,696 - CCNA_02011 0.30 -3.7 2,157,699 + CCNA_02011 0.31 +3.3 2,157,729 - CCNA_02011 0.38 -3.1 2,157,729 - CCNA_02011 0.38 -3.8 2,157,735 - CCNA_02011 0.39 -3.1 2,157,763 + CCNA_02011 0.46 +0.7 2,157,763 + CCNA_02011 0.46 +0.7 2,157,771 - CCNA_02011 0.48 -3.9 2,157,771 - CCNA_02011 0.48 -3.9 2,157,816 - CCNA_02011 0.59 -1.6 2,157,866 - CCNA_02011 0.70 -3.8 2,157,868 - CCNA_02011 0.71 -3.8 2,157,910 - CCNA_02011 0.81 -2.4 2,157,920 - CCNA_02011 0.83 -4.3 2,157,978 - -3.8 2,157,996 - +0.7 2,157,996 - +0.1 2,158,030 + +0.7 2,158,036 + +0.3 2,158,066 - -0.1 2,158,087 + +3.3 2,158,109 + +0.0 2,158,137 - -2.4 2,158,176 - -3.7 2,158,176 - -3.2 2,158,260 + +1.3 2,158,268 - CCNA_02012 0.10 -0.7 2,158,268 - CCNA_02012 0.10 -2.1 2,158,268 - CCNA_02012 0.10 -0.9 2,158,275 - CCNA_02012 0.11 -1.4 2,158,295 - CCNA_02012 0.12 -2.0 2,158,317 - CCNA_02012 0.13 -1.2 2,158,445 + CCNA_02012 0.21 -0.7 2,158,453 - CCNA_02012 0.21 -2.5 2,158,567 + CCNA_02012 0.28 +0.0
Or see this region's nucleotide sequence