Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20350

Experiment: Ammonium chloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20340 and BWI76_RS20345 are separated by 291 nucleotidesBWI76_RS20345 and BWI76_RS20350 are separated by 136 nucleotidesBWI76_RS20350 and BWI76_RS20355 are separated by 33 nucleotidesBWI76_RS20355 and BWI76_RS20360 are separated by 199 nucleotides BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841 _RS20340 BWI76_RS20345: BWI76_RS20345 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,259,133 to 4,259,702 _RS20345 BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356 _RS20350 BWI76_RS20355: BWI76_RS20355 - colicin V production protein, at 4,261,390 to 4,261,878 _RS20355 BWI76_RS20360: BWI76_RS20360 - cell division protein DedD, at 4,262,078 to 4,262,767 _RS20360 Position (kb) 4259 4260 4261 4262Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4258.848 kb on + strandat 4259.966 kb on + strandat 4259.966 kb on + strandat 4260.090 kb on + strand, within BWI76_RS20350at 4260.225 kb on + strand, within BWI76_RS20350at 4260.314 kb on - strand, within BWI76_RS20350at 4260.658 kb on + strand, within BWI76_RS20350at 4260.664 kb on - strand, within BWI76_RS20350at 4261.070 kb on - strand, within BWI76_RS20350at 4261.323 kb on + strandat 4261.470 kb on + strand, within BWI76_RS20355at 4261.551 kb on - strand, within BWI76_RS20355at 4261.570 kb on + strand, within BWI76_RS20355at 4262.158 kb on + strand, within BWI76_RS20360

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N)
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4,258,848 + -0.3
4,259,966 + -2.8
4,259,966 + -4.3
4,260,090 + BWI76_RS20350 0.17 -4.8
4,260,225 + BWI76_RS20350 0.25 -4.4
4,260,314 - BWI76_RS20350 0.31 -4.1
4,260,658 + BWI76_RS20350 0.54 -4.6
4,260,664 - BWI76_RS20350 0.54 -6.2
4,261,070 - BWI76_RS20350 0.81 -4.7
4,261,323 + -0.8
4,261,470 + BWI76_RS20355 0.16 -5.2
4,261,551 - BWI76_RS20355 0.33 -3.8
4,261,570 + BWI76_RS20355 0.37 -4.5
4,262,158 + BWI76_RS20360 0.12 -0.8

Or see this region's nucleotide sequence