Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00400 and BWI76_RS00405 are separated by 215 nucleotides BWI76_RS00405 and BWI76_RS00410 overlap by 48 nucleotides BWI76_RS00410 and BWI76_RS00415 are separated by 133 nucleotides BWI76_RS00415 and BWI76_RS00420 overlap by 7 nucleotides
BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943
_RS00400
BWI76_RS00405: BWI76_RS00405 - hypothetical protein, at 85,159 to 85,369
_RS00405
BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145
_RS00410
BWI76_RS00415: BWI76_RS00415 - glucose-1-phosphatase, at 87,279 to 87,878
_RS00415
BWI76_RS00420: BWI76_RS00420 - hypothetical protein, at 87,872 to 88,732
_RS00420
Position (kb) 85
86
87
88 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 84.616 kb on + strand, within BWI76_RS00400 at 84.953 kb on + strand at 84.961 kb on - strand at 85.041 kb on - strand at 85.289 kb on - strand, within BWI76_RS00405 at 87.087 kb on + strand at 87.269 kb on - strand at 87.414 kb on - strand, within BWI76_RS00415 at 87.492 kb on + strand, within BWI76_RS00415 at 87.523 kb on + strand, within BWI76_RS00415 at 87.543 kb on - strand, within BWI76_RS00415 at 87.604 kb on - strand, within BWI76_RS00415 at 87.614 kb on + strand, within BWI76_RS00415 at 87.772 kb on - strand, within BWI76_RS00415 at 87.796 kb on + strand, within BWI76_RS00415 at 87.804 kb on - strand, within BWI76_RS00415 at 87.882 kb on - strand at 88.062 kb on + strand, within BWI76_RS00420 at 88.140 kb on + strand, within BWI76_RS00420
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 84,616 + BWI76_RS00400 0.77 -3.0 84,953 + -3.0 84,961 - -3.3 85,041 - -4.1 85,289 - BWI76_RS00405 0.62 +0.4 87,087 + +0.0 87,269 - +0.7 87,414 - BWI76_RS00415 0.23 -0.7 87,492 + BWI76_RS00415 0.35 +1.2 87,523 + BWI76_RS00415 0.41 +1.5 87,543 - BWI76_RS00415 0.44 +0.0 87,604 - BWI76_RS00415 0.54 +0.7 87,614 + BWI76_RS00415 0.56 +0.5 87,772 - BWI76_RS00415 0.82 -1.1 87,796 + BWI76_RS00415 0.86 +0.6 87,804 - BWI76_RS00415 0.88 -1.5 87,882 - -0.4 88,062 + BWI76_RS00420 0.22 +0.9 88,140 + BWI76_RS00420 0.31 +1.3
Or see this region's nucleotide sequence