Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3367 and Dshi_3368 are separated by 2 nucleotides Dshi_3368 and Dshi_3369 are separated by 84 nucleotides Dshi_3369 and Dshi_3370 are separated by 64 nucleotides Dshi_3370 and Dshi_3371 are separated by 92 nucleotides
Dshi_3367: Dshi_3367 - 2-polyprenylphenol 6-hydroxylase (RefSeq), at 3,539,582 to 3,541,111
_3367
Dshi_3368: Dshi_3368 - ubiquinone/menaquinone biosynthesis methyltransferase (RefSeq), at 3,541,114 to 3,541,866
_3368
Dshi_3369: Dshi_3369 - formamidopyrimidine-DNA glycosylase (RefSeq), at 3,541,951 to 3,542,805
_3369
Dshi_3370: Dshi_3370 - Enoyl-CoA hydratase/isomerase (RefSeq), at 3,542,870 to 3,543,646
_3370
Dshi_3371: Dshi_3371 - ribosomal protein S20 (RefSeq), at 3,543,739 to 3,544,005
_3371
Position (kb)
3541
3542
3543 Strain fitness (log2 ratio)
-1
0
1
2 at 3541.886 kb on - strand at 3541.940 kb on + strand at 3541.942 kb on + strand at 3541.942 kb on + strand at 3541.976 kb on - strand at 3541.988 kb on + strand at 3542.044 kb on + strand, within Dshi_3369 at 3542.052 kb on - strand, within Dshi_3369 at 3542.067 kb on - strand, within Dshi_3369 at 3542.080 kb on - strand, within Dshi_3369 at 3542.147 kb on - strand, within Dshi_3369 at 3542.271 kb on - strand, within Dshi_3369 at 3542.293 kb on + strand, within Dshi_3369 at 3542.321 kb on + strand, within Dshi_3369 at 3542.326 kb on + strand, within Dshi_3369 at 3542.356 kb on + strand, within Dshi_3369 at 3542.438 kb on + strand, within Dshi_3369 at 3542.448 kb on + strand, within Dshi_3369 at 3542.456 kb on - strand, within Dshi_3369 at 3542.476 kb on - strand, within Dshi_3369 at 3542.512 kb on + strand, within Dshi_3369 at 3542.517 kb on + strand, within Dshi_3369 at 3542.525 kb on - strand, within Dshi_3369 at 3542.532 kb on - strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.602 kb on + strand, within Dshi_3369 at 3542.610 kb on - strand, within Dshi_3369 at 3542.610 kb on - strand, within Dshi_3369 at 3542.610 kb on - strand, within Dshi_3369 at 3542.610 kb on - strand, within Dshi_3369 at 3542.621 kb on + strand, within Dshi_3369 at 3542.665 kb on + strand, within Dshi_3369 at 3542.693 kb on + strand, within Dshi_3369 at 3542.751 kb on - strand at 3542.763 kb on + strand at 3542.771 kb on - strand at 3542.776 kb on + strand at 3542.795 kb on + strand at 3542.805 kb on + strand at 3542.813 kb on - strand at 3542.825 kb on + strand at 3543.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 3,541,886 - +0.0 3,541,940 + -0.2 3,541,942 + +0.3 3,541,942 + +0.7 3,541,976 - -0.0 3,541,988 + -0.7 3,542,044 + Dshi_3369 0.11 -0.2 3,542,052 - Dshi_3369 0.12 -0.1 3,542,067 - Dshi_3369 0.14 +0.8 3,542,080 - Dshi_3369 0.15 -0.5 3,542,147 - Dshi_3369 0.23 -0.9 3,542,271 - Dshi_3369 0.37 +0.7 3,542,293 + Dshi_3369 0.40 -0.4 3,542,321 + Dshi_3369 0.43 -0.4 3,542,326 + Dshi_3369 0.44 +0.5 3,542,356 + Dshi_3369 0.47 -0.5 3,542,438 + Dshi_3369 0.57 +0.1 3,542,448 + Dshi_3369 0.58 -0.3 3,542,456 - Dshi_3369 0.59 +0.4 3,542,476 - Dshi_3369 0.61 -0.7 3,542,512 + Dshi_3369 0.66 +0.2 3,542,517 + Dshi_3369 0.66 -0.7 3,542,525 - Dshi_3369 0.67 -0.4 3,542,532 - Dshi_3369 0.68 +0.3 3,542,602 + Dshi_3369 0.76 +0.4 3,542,602 + Dshi_3369 0.76 +0.7 3,542,602 + Dshi_3369 0.76 -0.2 3,542,602 + Dshi_3369 0.76 +0.7 3,542,602 + Dshi_3369 0.76 +0.1 3,542,602 + Dshi_3369 0.76 +0.1 3,542,602 + Dshi_3369 0.76 -0.3 3,542,610 - Dshi_3369 0.77 +0.3 3,542,610 - Dshi_3369 0.77 +1.3 3,542,610 - Dshi_3369 0.77 +0.3 3,542,610 - Dshi_3369 0.77 -0.5 3,542,621 + Dshi_3369 0.78 +0.4 3,542,665 + Dshi_3369 0.84 -0.5 3,542,693 + Dshi_3369 0.87 -1.2 3,542,751 - -0.7 3,542,763 + +1.9 3,542,771 - -0.3 3,542,776 + -0.9 3,542,795 + +0.8 3,542,805 + +1.3 3,542,813 - -0.7 3,542,825 + -0.1 3,543,655 - -0.3
Or see this region's nucleotide sequence