Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2671 and Dshi_2672 are separated by 174 nucleotides Dshi_2672 and Dshi_2673 are separated by 297 nucleotides Dshi_2673 and Dshi_2674 are separated by 357 nucleotides
Dshi_2671: Dshi_2671 - putative ABC transporter periplasmic solute-binding protein (RefSeq), at 2,826,918 to 2,827,889
_2671
Dshi_2672: Dshi_2672 - cytochrome c class I (RefSeq), at 2,828,064 to 2,828,483
_2672
Dshi_2673: Dshi_2673 - Pyrrolo-quinoline quinone (RefSeq), at 2,828,781 to 2,830,547
_2673
Dshi_2674: Dshi_2674 - two component transcriptional regulator, LuxR family (RefSeq), at 2,830,905 to 2,831,591
_2674
Position (kb)
2828
2829
2830
2831 Strain fitness (log2 ratio)
-2
-1
0
1 at 2827.783 kb on + strand, within Dshi_2671 at 2827.867 kb on + strand at 2827.874 kb on + strand at 2827.895 kb on - strand at 2827.917 kb on + strand at 2827.935 kb on + strand at 2827.944 kb on - strand at 2827.968 kb on + strand at 2828.014 kb on - strand at 2828.031 kb on + strand at 2828.039 kb on - strand at 2828.088 kb on + strand at 2828.088 kb on + strand at 2828.096 kb on - strand at 2828.108 kb on + strand, within Dshi_2672 at 2828.174 kb on + strand, within Dshi_2672 at 2828.182 kb on - strand, within Dshi_2672 at 2828.326 kb on + strand, within Dshi_2672 at 2828.402 kb on + strand, within Dshi_2672 at 2828.405 kb on + strand, within Dshi_2672 at 2828.420 kb on - strand, within Dshi_2672 at 2828.519 kb on - strand at 2828.576 kb on + strand at 2828.581 kb on + strand at 2828.594 kb on + strand at 2828.631 kb on - strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.728 kb on - strand at 2828.728 kb on - strand at 2828.729 kb on + strand at 2828.757 kb on + strand at 2828.757 kb on + strand at 2828.821 kb on + strand at 2828.853 kb on + strand at 2828.861 kb on - strand at 2828.867 kb on - strand at 2828.961 kb on + strand, within Dshi_2673 at 2828.986 kb on + strand, within Dshi_2673 at 2829.046 kb on + strand, within Dshi_2673 at 2829.056 kb on - strand, within Dshi_2673 at 2829.071 kb on - strand, within Dshi_2673 at 2829.154 kb on + strand, within Dshi_2673 at 2829.154 kb on + strand, within Dshi_2673 at 2829.157 kb on - strand, within Dshi_2673 at 2829.162 kb on - strand, within Dshi_2673 at 2829.162 kb on - strand, within Dshi_2673 at 2829.266 kb on - strand, within Dshi_2673 at 2829.290 kb on + strand, within Dshi_2673 at 2829.291 kb on + strand, within Dshi_2673 at 2829.555 kb on - strand, within Dshi_2673 at 2829.597 kb on + strand, within Dshi_2673 at 2829.605 kb on - strand, within Dshi_2673 at 2829.724 kb on + strand, within Dshi_2673 at 2829.743 kb on - strand, within Dshi_2673 at 2829.884 kb on + strand, within Dshi_2673 at 2829.887 kb on - strand, within Dshi_2673 at 2829.894 kb on + strand, within Dshi_2673 at 2829.918 kb on + strand, within Dshi_2673 at 2830.272 kb on + strand, within Dshi_2673 at 2830.286 kb on - strand, within Dshi_2673 at 2830.294 kb on + strand, within Dshi_2673 at 2830.426 kb on + strand at 2830.494 kb on - strand at 2830.634 kb on - strand at 2830.764 kb on - strand at 2830.973 kb on + strand at 2830.981 kb on - strand, within Dshi_2674 at 2831.141 kb on + strand, within Dshi_2674 at 2831.147 kb on - strand, within Dshi_2674 at 2831.289 kb on + strand, within Dshi_2674 at 2831.297 kb on - strand, within Dshi_2674 at 2831.297 kb on - strand, within Dshi_2674 at 2831.345 kb on + strand, within Dshi_2674 at 2831.359 kb on - strand, within Dshi_2674 at 2831.373 kb on - strand, within Dshi_2674 at 2831.440 kb on + strand, within Dshi_2674 at 2831.447 kb on - strand, within Dshi_2674 at 2831.516 kb on - strand, within Dshi_2674
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 2,827,783 + Dshi_2671 0.89 +0.6 2,827,867 + +0.4 2,827,874 + -0.5 2,827,895 - -0.9 2,827,917 + -0.6 2,827,935 + +0.3 2,827,944 - -0.7 2,827,968 + -0.1 2,828,014 - -0.2 2,828,031 + -0.3 2,828,039 - -0.7 2,828,088 + -0.1 2,828,088 + -0.4 2,828,096 - -0.2 2,828,108 + Dshi_2672 0.10 +0.8 2,828,174 + Dshi_2672 0.26 -0.8 2,828,182 - Dshi_2672 0.28 +0.7 2,828,326 + Dshi_2672 0.62 -0.4 2,828,402 + Dshi_2672 0.80 +0.3 2,828,405 + Dshi_2672 0.81 +0.2 2,828,420 - Dshi_2672 0.85 +0.1 2,828,519 - -0.0 2,828,576 + -1.1 2,828,581 + +0.8 2,828,594 + -0.5 2,828,631 - -0.7 2,828,720 + +0.1 2,828,720 + -0.1 2,828,720 + -0.1 2,828,720 + -0.5 2,828,728 - -0.0 2,828,728 - -0.3 2,828,729 + +0.2 2,828,757 + -0.4 2,828,757 + -0.3 2,828,821 + +0.9 2,828,853 + +0.1 2,828,861 - -0.5 2,828,867 - -1.0 2,828,961 + Dshi_2673 0.10 -0.1 2,828,986 + Dshi_2673 0.12 -0.1 2,829,046 + Dshi_2673 0.15 -1.1 2,829,056 - Dshi_2673 0.16 -0.5 2,829,071 - Dshi_2673 0.16 +0.2 2,829,154 + Dshi_2673 0.21 +0.7 2,829,154 + Dshi_2673 0.21 +0.0 2,829,157 - Dshi_2673 0.21 -0.2 2,829,162 - Dshi_2673 0.22 -1.9 2,829,162 - Dshi_2673 0.22 -1.1 2,829,266 - Dshi_2673 0.27 -0.1 2,829,290 + Dshi_2673 0.29 -0.3 2,829,291 + Dshi_2673 0.29 -0.7 2,829,555 - Dshi_2673 0.44 -0.8 2,829,597 + Dshi_2673 0.46 +0.2 2,829,605 - Dshi_2673 0.47 +0.5 2,829,724 + Dshi_2673 0.53 -0.1 2,829,743 - Dshi_2673 0.54 -0.5 2,829,884 + Dshi_2673 0.62 -0.1 2,829,887 - Dshi_2673 0.63 -0.3 2,829,894 + Dshi_2673 0.63 -0.5 2,829,918 + Dshi_2673 0.64 -0.7 2,830,272 + Dshi_2673 0.84 +0.6 2,830,286 - Dshi_2673 0.85 -0.2 2,830,294 + Dshi_2673 0.86 -1.1 2,830,426 + +0.4 2,830,494 - -0.3 2,830,634 - +0.4 2,830,764 - -0.2 2,830,973 + +0.6 2,830,981 - Dshi_2674 0.11 -0.1 2,831,141 + Dshi_2674 0.34 +0.0 2,831,147 - Dshi_2674 0.35 -0.3 2,831,289 + Dshi_2674 0.56 -1.0 2,831,297 - Dshi_2674 0.57 -0.1 2,831,297 - Dshi_2674 0.57 -1.3 2,831,345 + Dshi_2674 0.64 +0.4 2,831,359 - Dshi_2674 0.66 +0.8 2,831,373 - Dshi_2674 0.68 +0.1 2,831,440 + Dshi_2674 0.78 +0.2 2,831,447 - Dshi_2674 0.79 +0.7 2,831,516 - Dshi_2674 0.89 -1.1
Or see this region's nucleotide sequence