Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2289 and Dshi_2290 are separated by 8 nucleotides Dshi_2290 and Dshi_2291 are separated by 33 nucleotides Dshi_2291 and Dshi_2292 are separated by 143 nucleotides
Dshi_2289: Dshi_2289 - amidohydrolase (RefSeq), at 2,418,278 to 2,419,648
_2289
Dshi_2290: Dshi_2290 - Carbohydrate-selective porin OprB (RefSeq), at 2,419,657 to 2,420,955
_2290
Dshi_2291: Dshi_2291 - protein of unknown function DUF306 Meta and HslJ (RefSeq), at 2,420,989 to 2,421,627
_2291
Dshi_2292: Dshi_2292 - hypothetical protein (RefSeq), at 2,421,771 to 2,422,820
_2292
Position (kb)
2419
2420
2421 Strain fitness (log2 ratio)
-1
0
1 at 2418.851 kb on - strand, within Dshi_2289 at 2418.856 kb on - strand, within Dshi_2289 at 2418.900 kb on + strand, within Dshi_2289 at 2418.937 kb on - strand, within Dshi_2289 at 2418.937 kb on - strand, within Dshi_2289 at 2418.937 kb on - strand, within Dshi_2289 at 2419.075 kb on + strand, within Dshi_2289 at 2419.095 kb on + strand, within Dshi_2289 at 2419.137 kb on + strand, within Dshi_2289 at 2419.145 kb on - strand, within Dshi_2289 at 2419.180 kb on + strand, within Dshi_2289 at 2419.181 kb on - strand, within Dshi_2289 at 2419.210 kb on - strand, within Dshi_2289 at 2419.211 kb on - strand, within Dshi_2289 at 2419.244 kb on - strand, within Dshi_2289 at 2419.318 kb on - strand, within Dshi_2289 at 2419.404 kb on + strand, within Dshi_2289 at 2419.406 kb on + strand, within Dshi_2289 at 2419.414 kb on - strand, within Dshi_2289 at 2419.414 kb on - strand, within Dshi_2289 at 2419.414 kb on - strand, within Dshi_2289 at 2419.452 kb on + strand, within Dshi_2289 at 2419.454 kb on + strand, within Dshi_2289 at 2419.494 kb on + strand, within Dshi_2289 at 2419.530 kb on + strand at 2419.722 kb on - strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.770 kb on - strand at 2419.799 kb on - strand, within Dshi_2290 at 2419.802 kb on + strand, within Dshi_2290 at 2419.827 kb on - strand, within Dshi_2290 at 2419.827 kb on - strand, within Dshi_2290 at 2419.849 kb on + strand, within Dshi_2290 at 2419.884 kb on + strand, within Dshi_2290 at 2419.978 kb on - strand, within Dshi_2290 at 2419.978 kb on - strand, within Dshi_2290 at 2420.015 kb on + strand, within Dshi_2290 at 2420.075 kb on + strand, within Dshi_2290 at 2420.110 kb on - strand, within Dshi_2290 at 2420.215 kb on + strand, within Dshi_2290 at 2420.243 kb on + strand, within Dshi_2290 at 2420.354 kb on - strand, within Dshi_2290 at 2420.354 kb on - strand, within Dshi_2290 at 2420.475 kb on + strand, within Dshi_2290 at 2420.518 kb on - strand, within Dshi_2290 at 2420.574 kb on + strand, within Dshi_2290 at 2420.582 kb on - strand, within Dshi_2290 at 2420.623 kb on - strand, within Dshi_2290 at 2420.735 kb on + strand, within Dshi_2290 at 2420.735 kb on + strand, within Dshi_2290 at 2420.750 kb on + strand, within Dshi_2290 at 2420.750 kb on + strand, within Dshi_2290 at 2420.758 kb on - strand, within Dshi_2290 at 2420.763 kb on - strand, within Dshi_2290 at 2420.824 kb on - strand, within Dshi_2290 at 2420.902 kb on - strand at 2420.903 kb on - strand at 2420.928 kb on - strand at 2421.123 kb on + strand, within Dshi_2291 at 2421.128 kb on + strand, within Dshi_2291 at 2421.128 kb on + strand, within Dshi_2291 at 2421.128 kb on + strand, within Dshi_2291 at 2421.128 kb on + strand, within Dshi_2291 at 2421.136 kb on - strand, within Dshi_2291 at 2421.449 kb on - strand, within Dshi_2291 at 2421.552 kb on - strand, within Dshi_2291 at 2421.554 kb on + strand, within Dshi_2291 at 2421.554 kb on + strand, within Dshi_2291 at 2421.622 kb on + strand at 2421.622 kb on - strand at 2421.630 kb on - strand at 2421.637 kb on + strand at 2421.659 kb on - strand at 2421.671 kb on + strand at 2421.679 kb on - strand at 2421.679 kb on - strand at 2421.696 kb on - strand at 2421.856 kb on + strand at 2421.893 kb on - strand, within Dshi_2292 at 2421.908 kb on - strand, within Dshi_2292 at 2421.940 kb on + strand, within Dshi_2292
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 2,418,851 - Dshi_2289 0.42 -1.3 2,418,856 - Dshi_2289 0.42 -0.2 2,418,900 + Dshi_2289 0.45 -0.7 2,418,937 - Dshi_2289 0.48 -0.9 2,418,937 - Dshi_2289 0.48 +0.1 2,418,937 - Dshi_2289 0.48 +0.5 2,419,075 + Dshi_2289 0.58 -0.9 2,419,095 + Dshi_2289 0.60 -0.5 2,419,137 + Dshi_2289 0.63 +0.7 2,419,145 - Dshi_2289 0.63 -0.2 2,419,180 + Dshi_2289 0.66 -0.1 2,419,181 - Dshi_2289 0.66 -0.1 2,419,210 - Dshi_2289 0.68 -0.2 2,419,211 - Dshi_2289 0.68 -0.5 2,419,244 - Dshi_2289 0.70 -0.2 2,419,318 - Dshi_2289 0.76 +0.8 2,419,404 + Dshi_2289 0.82 +0.2 2,419,406 + Dshi_2289 0.82 +1.1 2,419,414 - Dshi_2289 0.83 -0.2 2,419,414 - Dshi_2289 0.83 +1.1 2,419,414 - Dshi_2289 0.83 -0.4 2,419,452 + Dshi_2289 0.86 -0.6 2,419,454 + Dshi_2289 0.86 -1.2 2,419,494 + Dshi_2289 0.89 -0.4 2,419,530 + +0.8 2,419,722 - +1.7 2,419,754 - +1.1 2,419,754 - -0.7 2,419,770 - +0.5 2,419,799 - Dshi_2290 0.11 -0.3 2,419,802 + Dshi_2290 0.11 +0.6 2,419,827 - Dshi_2290 0.13 +0.3 2,419,827 - Dshi_2290 0.13 +0.5 2,419,849 + Dshi_2290 0.15 -0.3 2,419,884 + Dshi_2290 0.17 -0.1 2,419,978 - Dshi_2290 0.25 -0.7 2,419,978 - Dshi_2290 0.25 -0.1 2,420,015 + Dshi_2290 0.28 +0.1 2,420,075 + Dshi_2290 0.32 -0.3 2,420,110 - Dshi_2290 0.35 -0.3 2,420,215 + Dshi_2290 0.43 +0.5 2,420,243 + Dshi_2290 0.45 -0.2 2,420,354 - Dshi_2290 0.54 +0.6 2,420,354 - Dshi_2290 0.54 +0.6 2,420,475 + Dshi_2290 0.63 -0.0 2,420,518 - Dshi_2290 0.66 -0.0 2,420,574 + Dshi_2290 0.71 -1.2 2,420,582 - Dshi_2290 0.71 -0.4 2,420,623 - Dshi_2290 0.74 +0.7 2,420,735 + Dshi_2290 0.83 +0.8 2,420,735 + Dshi_2290 0.83 +0.5 2,420,750 + Dshi_2290 0.84 +0.5 2,420,750 + Dshi_2290 0.84 -0.0 2,420,758 - Dshi_2290 0.85 +0.3 2,420,763 - Dshi_2290 0.85 +1.6 2,420,824 - Dshi_2290 0.90 +0.0 2,420,902 - +0.1 2,420,903 - -0.6 2,420,928 - +0.9 2,421,123 + Dshi_2291 0.21 -0.1 2,421,128 + Dshi_2291 0.22 -0.4 2,421,128 + Dshi_2291 0.22 +0.5 2,421,128 + Dshi_2291 0.22 +0.1 2,421,128 + Dshi_2291 0.22 -0.9 2,421,136 - Dshi_2291 0.23 -0.5 2,421,449 - Dshi_2291 0.72 +0.3 2,421,552 - Dshi_2291 0.88 -0.1 2,421,554 + Dshi_2291 0.88 -0.4 2,421,554 + Dshi_2291 0.88 -0.3 2,421,622 + +0.2 2,421,622 - +0.1 2,421,630 - +1.1 2,421,637 + -0.4 2,421,659 - -0.5 2,421,671 + +0.3 2,421,679 - -0.8 2,421,679 - -0.7 2,421,696 - +0.1 2,421,856 + -0.3 2,421,893 - Dshi_2292 0.12 -1.4 2,421,908 - Dshi_2292 0.13 +0.3 2,421,940 + Dshi_2292 0.16 +0.4
Or see this region's nucleotide sequence