Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2174 and Dshi_2175 overlap by 4 nucleotides Dshi_2175 and Dshi_2176 are separated by 12 nucleotides Dshi_2176 and Dshi_2177 overlap by 10 nucleotides Dshi_2177 and Dshi_2178 are separated by 107 nucleotides
Dshi_2174: Dshi_2174 - phage major capsid protein, HK97 family (RefSeq), at 2,301,398 to 2,302,615
_2174
Dshi_2175: Dshi_2175 - phage prohead protease, HK97 family (RefSeq), at 2,302,612 to 2,303,229
_2175
Dshi_2176: Dshi_2176 - hypothetical protein (RefSeq), at 2,303,242 to 2,303,508
_2176
Dshi_2177: Dshi_2177 - phage portal protein, HK97 family (RefSeq), at 2,303,499 to 2,304,689
_2177
Dshi_2178: Dshi_2178 - protein of unknown function DUF264 (RefSeq), at 2,304,797 to 2,306,155
_2178
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-1
0
1
2 at 2302.527 kb on - strand at 2302.599 kb on - strand at 2302.651 kb on - strand at 2302.670 kb on - strand at 2302.673 kb on + strand at 2302.777 kb on - strand, within Dshi_2175 at 2302.943 kb on + strand, within Dshi_2175 at 2302.981 kb on + strand, within Dshi_2175 at 2302.986 kb on + strand, within Dshi_2175 at 2303.106 kb on + strand, within Dshi_2175 at 2303.114 kb on - strand, within Dshi_2175 at 2303.118 kb on - strand, within Dshi_2175 at 2303.160 kb on - strand, within Dshi_2175 at 2303.195 kb on + strand at 2303.293 kb on - strand, within Dshi_2176 at 2303.439 kb on - strand, within Dshi_2176 at 2303.477 kb on + strand, within Dshi_2176 at 2303.498 kb on + strand at 2303.498 kb on + strand at 2303.549 kb on - strand at 2303.588 kb on - strand at 2303.618 kb on - strand, within Dshi_2177 at 2303.669 kb on + strand, within Dshi_2177 at 2303.706 kb on + strand, within Dshi_2177 at 2303.721 kb on - strand, within Dshi_2177 at 2303.831 kb on + strand, within Dshi_2177 at 2303.890 kb on + strand, within Dshi_2177 at 2303.973 kb on - strand, within Dshi_2177 at 2304.053 kb on - strand, within Dshi_2177 at 2304.064 kb on - strand, within Dshi_2177 at 2304.151 kb on - strand, within Dshi_2177 at 2304.206 kb on - strand, within Dshi_2177 at 2304.214 kb on + strand, within Dshi_2177 at 2304.221 kb on + strand, within Dshi_2177 at 2304.229 kb on - strand, within Dshi_2177 at 2304.476 kb on - strand, within Dshi_2177 at 2304.574 kb on - strand at 2304.594 kb on - strand at 2304.675 kb on - strand at 2304.809 kb on + strand at 2304.809 kb on + strand at 2304.817 kb on - strand at 2304.852 kb on - strand at 2304.861 kb on + strand at 2304.869 kb on - strand at 2304.984 kb on - strand, within Dshi_2178 at 2305.085 kb on - strand, within Dshi_2178 at 2305.085 kb on - strand, within Dshi_2178 at 2305.099 kb on + strand, within Dshi_2178 at 2305.120 kb on + strand, within Dshi_2178 at 2305.278 kb on - strand, within Dshi_2178 at 2305.286 kb on - strand, within Dshi_2178 at 2305.346 kb on - strand, within Dshi_2178 at 2305.347 kb on + strand, within Dshi_2178 at 2305.663 kb on + strand, within Dshi_2178 at 2305.681 kb on - strand, within Dshi_2178
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 2,302,527 - -0.6 2,302,599 - -1.0 2,302,651 - -0.2 2,302,670 - +1.8 2,302,673 + -0.4 2,302,777 - Dshi_2175 0.27 +0.1 2,302,943 + Dshi_2175 0.54 -0.5 2,302,981 + Dshi_2175 0.60 +0.3 2,302,986 + Dshi_2175 0.61 -0.2 2,303,106 + Dshi_2175 0.80 +0.4 2,303,114 - Dshi_2175 0.81 -0.4 2,303,118 - Dshi_2175 0.82 -0.1 2,303,160 - Dshi_2175 0.89 -0.0 2,303,195 + +0.2 2,303,293 - Dshi_2176 0.19 -1.2 2,303,439 - Dshi_2176 0.74 +0.6 2,303,477 + Dshi_2176 0.88 -0.3 2,303,498 + -0.0 2,303,498 + +0.5 2,303,549 - -0.5 2,303,588 - -1.2 2,303,618 - Dshi_2177 0.10 -0.2 2,303,669 + Dshi_2177 0.14 -0.5 2,303,706 + Dshi_2177 0.17 +0.4 2,303,721 - Dshi_2177 0.19 +0.2 2,303,831 + Dshi_2177 0.28 +0.6 2,303,890 + Dshi_2177 0.33 +0.3 2,303,973 - Dshi_2177 0.40 -0.6 2,304,053 - Dshi_2177 0.47 +0.3 2,304,064 - Dshi_2177 0.47 +1.0 2,304,151 - Dshi_2177 0.55 +0.4 2,304,206 - Dshi_2177 0.59 +0.7 2,304,214 + Dshi_2177 0.60 +0.4 2,304,221 + Dshi_2177 0.61 +0.7 2,304,229 - Dshi_2177 0.61 -0.1 2,304,476 - Dshi_2177 0.82 +0.1 2,304,574 - -0.2 2,304,594 - -0.2 2,304,675 - -1.6 2,304,809 + +0.8 2,304,809 + +0.3 2,304,817 - -0.4 2,304,852 - -0.7 2,304,861 + +0.2 2,304,869 - +0.0 2,304,984 - Dshi_2178 0.14 -0.3 2,305,085 - Dshi_2178 0.21 +0.5 2,305,085 - Dshi_2178 0.21 +0.6 2,305,099 + Dshi_2178 0.22 -0.4 2,305,120 + Dshi_2178 0.24 -0.1 2,305,278 - Dshi_2178 0.35 +0.1 2,305,286 - Dshi_2178 0.36 -0.6 2,305,346 - Dshi_2178 0.40 -0.3 2,305,347 + Dshi_2178 0.40 -0.5 2,305,663 + Dshi_2178 0.64 -0.3 2,305,681 - Dshi_2178 0.65 -0.6
Or see this region's nucleotide sequence