Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1460 and Dshi_1461 are separated by 15 nucleotides Dshi_1461 and Dshi_1462 are separated by 78 nucleotides Dshi_1462 and Dshi_1463 overlap by 4 nucleotides Dshi_1463 and Dshi_1464 are separated by 4 nucleotides
Dshi_1460: Dshi_1460 - ribosomal protein L21 (RefSeq), at 1,517,692 to 1,518,357
_1460
Dshi_1461: Dshi_1461 - ribosomal protein L27 (RefSeq), at 1,518,373 to 1,518,642
_1461
Dshi_1462: Dshi_1462 - LysE family transport protein (RefSeq), at 1,518,721 to 1,519,377
_1462
Dshi_1463: Dshi_1463 - GCN5-related N-acetyltransferase (RefSeq), at 1,519,374 to 1,519,913
_1463
Dshi_1464: Dshi_1464 - GCN5-related N-acetyltransferase (RefSeq), at 1,519,918 to 1,520,427
_1464
Position (kb)
1518
1519
1520 Strain fitness (log2 ratio)
-1
0
1 at 1518.766 kb on + strand at 1518.873 kb on + strand, within Dshi_1462 at 1519.105 kb on - strand, within Dshi_1462 at 1519.263 kb on + strand, within Dshi_1462 at 1519.324 kb on + strand at 1519.518 kb on + strand, within Dshi_1463 at 1520.030 kb on + strand, within Dshi_1464 at 1520.117 kb on - strand, within Dshi_1464 at 1520.197 kb on + strand, within Dshi_1464 at 1520.205 kb on - strand, within Dshi_1464
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 1,518,766 + -0.3 1,518,873 + Dshi_1462 0.23 -0.7 1,519,105 - Dshi_1462 0.58 -1.4 1,519,263 + Dshi_1462 0.82 +0.0 1,519,324 + -0.3 1,519,518 + Dshi_1463 0.27 -0.8 1,520,030 + Dshi_1464 0.22 +0.9 1,520,117 - Dshi_1464 0.39 +0.4 1,520,197 + Dshi_1464 0.55 +0.2 1,520,205 - Dshi_1464 0.56 +0.1
Or see this region's nucleotide sequence