Experiment: m.b. Spectinomycin 0.0015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0434 and Dshi_0435 are separated by 253 nucleotides Dshi_0435 and Dshi_0436 overlap by 4 nucleotides Dshi_0436 and Dshi_0437 overlap by 4 nucleotides Dshi_0437 and Dshi_0438 overlap by 4 nucleotides Dshi_0438 and Dshi_0439 overlap by 10 nucleotides
Dshi_0434: Dshi_0434 - carbamate kinase (RefSeq), at 425,925 to 426,863
_0434
Dshi_0435: Dshi_0435 - ATP synthase F1, beta subunit (RefSeq), at 427,117 to 428,505
_0435
Dshi_0436: Dshi_0436 - ATP synthase F1, epsilon subunit (RefSeq), at 428,502 to 428,912
_0436
Dshi_0437: Dshi_0437 - F0F1-ATPase subunit, putative (RefSeq), at 428,909 to 429,196
_0437
Dshi_0438: Dshi_0438 - hypothetical protein (RefSeq), at 429,193 to 429,498
_0438
Dshi_0439: Dshi_0439 - ATP synthase F0, A subunit (RefSeq), at 429,489 to 430,178
_0439
Position (kb) 427
428
429 Strain fitness (log2 ratio) -2
-1
0
1
2 at 426.213 kb on + strand, within Dshi_0434 at 426.213 kb on + strand, within Dshi_0434 at 426.221 kb on - strand, within Dshi_0434 at 426.242 kb on + strand, within Dshi_0434 at 426.350 kb on - strand, within Dshi_0434 at 426.363 kb on + strand, within Dshi_0434 at 426.456 kb on - strand, within Dshi_0434 at 426.456 kb on - strand, within Dshi_0434 at 426.856 kb on - strand at 426.882 kb on + strand at 426.882 kb on + strand at 426.974 kb on + strand at 426.998 kb on - strand at 427.021 kb on + strand at 427.107 kb on + strand at 427.113 kb on + strand at 427.135 kb on + strand at 427.155 kb on - strand at 427.289 kb on + strand, within Dshi_0435 at 427.415 kb on - strand, within Dshi_0435 at 427.498 kb on + strand, within Dshi_0435 at 427.561 kb on + strand, within Dshi_0435 at 427.564 kb on - strand, within Dshi_0435 at 427.582 kb on - strand at 427.640 kb on + strand, within Dshi_0435 at 427.650 kb on + strand, within Dshi_0435 at 427.708 kb on - strand, within Dshi_0435 at 427.709 kb on + strand, within Dshi_0435 at 427.738 kb on - strand, within Dshi_0435 at 427.823 kb on + strand, within Dshi_0435 at 427.842 kb on - strand, within Dshi_0435 at 427.897 kb on - strand, within Dshi_0435 at 427.960 kb on + strand, within Dshi_0435 at 428.071 kb on + strand, within Dshi_0435 at 428.113 kb on - strand, within Dshi_0435 at 428.114 kb on + strand, within Dshi_0435 at 428.139 kb on + strand, within Dshi_0435 at 428.162 kb on + strand, within Dshi_0435 at 428.209 kb on + strand, within Dshi_0435 at 428.264 kb on - strand, within Dshi_0435 at 428.278 kb on + strand, within Dshi_0435 at 428.278 kb on + strand, within Dshi_0435 at 428.286 kb on - strand, within Dshi_0435 at 428.312 kb on + strand, within Dshi_0435 at 428.405 kb on + strand at 428.530 kb on + strand at 428.571 kb on - strand, within Dshi_0436 at 428.781 kb on - strand, within Dshi_0436 at 428.831 kb on - strand, within Dshi_0436 at 428.957 kb on + strand, within Dshi_0437 at 429.079 kb on - strand, within Dshi_0437 at 429.183 kb on - strand at 429.273 kb on + strand, within Dshi_0438 at 429.281 kb on - strand, within Dshi_0438 at 429.330 kb on + strand, within Dshi_0438 at 429.332 kb on + strand, within Dshi_0438 at 429.342 kb on + strand, within Dshi_0438 at 429.411 kb on - strand, within Dshi_0438 at 429.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.0015 mg/ml remove 426,213 + Dshi_0434 0.31 +0.2 426,213 + Dshi_0434 0.31 +0.6 426,221 - Dshi_0434 0.32 +0.4 426,242 + Dshi_0434 0.34 +0.2 426,350 - Dshi_0434 0.45 +0.2 426,363 + Dshi_0434 0.47 +0.1 426,456 - Dshi_0434 0.57 +0.5 426,456 - Dshi_0434 0.57 -0.6 426,856 - -0.4 426,882 + -0.6 426,882 + +0.1 426,974 + +0.0 426,998 - +1.2 427,021 + -0.1 427,107 + +0.6 427,113 + -0.3 427,135 + -2.5 427,155 - +1.2 427,289 + Dshi_0435 0.12 -0.2 427,415 - Dshi_0435 0.21 -0.4 427,498 + Dshi_0435 0.27 +1.2 427,561 + Dshi_0435 0.32 +0.0 427,564 - Dshi_0435 0.32 +0.7 427,582 - +0.6 427,640 + Dshi_0435 0.38 -0.0 427,650 + Dshi_0435 0.38 -0.4 427,708 - Dshi_0435 0.43 -0.5 427,709 + Dshi_0435 0.43 +0.3 427,738 - Dshi_0435 0.45 +0.5 427,823 + Dshi_0435 0.51 +1.8 427,842 - Dshi_0435 0.52 -0.1 427,897 - Dshi_0435 0.56 -0.3 427,960 + Dshi_0435 0.61 +0.5 428,071 + Dshi_0435 0.69 -0.2 428,113 - Dshi_0435 0.72 +0.2 428,114 + Dshi_0435 0.72 +0.1 428,139 + Dshi_0435 0.74 -0.6 428,162 + Dshi_0435 0.75 +0.2 428,209 + Dshi_0435 0.79 -0.4 428,264 - Dshi_0435 0.83 +0.1 428,278 + Dshi_0435 0.84 +0.5 428,278 + Dshi_0435 0.84 +0.4 428,286 - Dshi_0435 0.84 +1.8 428,312 + Dshi_0435 0.86 +0.2 428,405 + +0.1 428,530 + +0.2 428,571 - Dshi_0436 0.17 -0.9 428,781 - Dshi_0436 0.68 +0.0 428,831 - Dshi_0436 0.80 -0.1 428,957 + Dshi_0437 0.17 +0.7 429,079 - Dshi_0437 0.59 -0.3 429,183 - -0.1 429,273 + Dshi_0438 0.26 -1.5 429,281 - Dshi_0438 0.29 +0.2 429,330 + Dshi_0438 0.45 +0.5 429,332 + Dshi_0438 0.45 -1.2 429,342 + Dshi_0438 0.49 +0.1 429,411 - Dshi_0438 0.71 -0.3 429,492 + -0.3
Or see this region's nucleotide sequence