Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2389 and Dsui_2390 are separated by 101 nucleotides Dsui_2390 and Dsui_2391 overlap by 4 nucleotides Dsui_2391 and Dsui_2392 are separated by 2 nucleotides
Dsui_2389: Dsui_2389 - putative membrane protein, at 2,541,439 to 2,542,710
_2389
Dsui_2390: Dsui_2390 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,542,812 to 2,543,528
_2390
Dsui_2391: Dsui_2391 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,543,525 to 2,544,013
_2391
Dsui_2392: Dsui_2392 - 2''-5'' RNA ligase, at 2,544,016 to 2,544,591
_2392
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2541.931 kb on - strand at 2541.960 kb on + strand at 2541.997 kb on - strand, within Dsui_2389 at 2542.019 kb on + strand at 2542.034 kb on + strand at 2542.092 kb on - strand, within Dsui_2389 at 2542.149 kb on - strand, within Dsui_2389 at 2542.181 kb on - strand, within Dsui_2389 at 2542.218 kb on - strand, within Dsui_2389 at 2542.345 kb on - strand, within Dsui_2389 at 2542.374 kb on + strand, within Dsui_2389 at 2542.430 kb on - strand at 2542.579 kb on - strand, within Dsui_2389 at 2542.583 kb on - strand at 2542.583 kb on - strand at 2542.629 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.786 kb on - strand at 2544.017 kb on + strand at 2544.162 kb on - strand at 2544.192 kb on + strand, within Dsui_2392 at 2544.221 kb on - strand at 2544.255 kb on - strand at 2544.255 kb on - strand at 2544.315 kb on + strand, within Dsui_2392 at 2544.524 kb on + strand, within Dsui_2392
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,541,931 - +0.8 2,541,960 + +0.8 2,541,997 - Dsui_2389 0.44 -1.5 2,542,019 + +0.8 2,542,034 + -0.2 2,542,092 - Dsui_2389 0.51 +0.8 2,542,149 - Dsui_2389 0.56 -0.2 2,542,181 - Dsui_2389 0.58 +0.8 2,542,218 - Dsui_2389 0.61 -1.2 2,542,345 - Dsui_2389 0.71 +0.8 2,542,374 + Dsui_2389 0.74 -0.2 2,542,430 - +0.8 2,542,579 - Dsui_2389 0.90 -2.9 2,542,583 - +0.8 2,542,583 - -0.2 2,542,629 - +0.8 2,542,652 - -1.5 2,542,652 - +0.8 2,542,652 - +0.8 2,542,652 - -0.2 2,542,652 - +0.1 2,542,652 - +0.8 2,542,652 - +0.8 2,542,786 - +0.8 2,544,017 + -0.3 2,544,162 - -0.2 2,544,192 + Dsui_2392 0.31 -0.0 2,544,221 - +0.8 2,544,255 - +0.8 2,544,255 - -0.2 2,544,315 + Dsui_2392 0.52 -0.5 2,544,524 + Dsui_2392 0.88 -0.5
Or see this region's nucleotide sequence