Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0169 and Dsui_0170 overlap by 4 nucleotides Dsui_0170 and Dsui_0171 are separated by 555 nucleotides Dsui_0171 and Dsui_0172 overlap by 4 nucleotides
Dsui_0169: Dsui_0169 - anthranilate phosphoribosyltransferase, at 172,705 to 173,727
_0169
Dsui_0170: Dsui_0170 - glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase, at 173,724 to 174,296
_0170
Dsui_0171: Dsui_0171 - putative transcriptional regulator, at 174,852 to 175,070
_0171
Dsui_0172: Dsui_0172 - hypothetical protein, at 175,067 to 175,741
_0172
Position (kb)
173
174
175 Strain fitness (log2 ratio)
-2
-1
0
1 at 173.733 kb on - strand at 173.733 kb on - strand at 174.500 kb on + strand at 174.530 kb on + strand at 174.536 kb on + strand at 174.536 kb on + strand at 174.544 kb on - strand at 174.544 kb on - strand at 174.544 kb on - strand at 174.544 kb on - strand at 174.544 kb on - strand at 174.566 kb on + strand at 174.595 kb on + strand at 174.663 kb on + strand at 174.666 kb on - strand at 174.671 kb on - strand at 174.676 kb on - strand at 174.676 kb on - strand at 174.697 kb on + strand at 174.753 kb on + strand at 174.780 kb on - strand at 174.780 kb on - strand at 174.785 kb on - strand at 174.822 kb on + strand at 174.823 kb on - strand at 174.823 kb on - strand at 174.955 kb on + strand, within Dsui_0171 at 174.982 kb on + strand, within Dsui_0171 at 174.990 kb on - strand, within Dsui_0171 at 175.018 kb on - strand, within Dsui_0171 at 175.028 kb on + strand, within Dsui_0171 at 175.041 kb on - strand, within Dsui_0171 at 175.074 kb on - strand at 175.122 kb on + strand at 175.122 kb on + strand at 175.123 kb on + strand at 175.212 kb on + strand, within Dsui_0172 at 175.215 kb on - strand, within Dsui_0172 at 175.238 kb on + strand at 175.246 kb on - strand, within Dsui_0172 at 175.257 kb on - strand at 175.274 kb on + strand, within Dsui_0172
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 173,733 - +0.3 173,733 - -0.1 174,500 + +0.7 174,530 + +0.7 174,536 + -0.3 174,536 + -0.3 174,544 - +0.7 174,544 - +0.7 174,544 - -0.9 174,544 - -0.3 174,544 - -0.3 174,566 + -2.1 174,595 + +0.7 174,663 + -0.9 174,666 - -0.3 174,671 - -1.3 174,676 - +0.7 174,676 - -1.6 174,697 + +0.7 174,753 + -0.3 174,780 - +0.7 174,780 - -0.3 174,785 - -0.3 174,822 + +0.7 174,823 - -1.6 174,823 - +0.7 174,955 + Dsui_0171 0.47 -0.3 174,982 + Dsui_0171 0.59 -0.3 174,990 - Dsui_0171 0.63 -1.6 175,018 - Dsui_0171 0.76 -1.6 175,028 + Dsui_0171 0.80 -1.9 175,041 - Dsui_0171 0.86 -2.1 175,074 - -0.3 175,122 + -0.5 175,122 + -0.5 175,123 + -1.1 175,212 + Dsui_0172 0.21 -0.3 175,215 - Dsui_0172 0.22 -2.1 175,238 + -0.3 175,246 - Dsui_0172 0.27 -0.9 175,257 - +0.7 175,274 + Dsui_0172 0.31 +0.7
Or see this region's nucleotide sequence