Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0965 and BT0966 are separated by 63 nucleotides BT0966 and BT0967 are separated by 94 nucleotides BT0967 and BT0968 are separated by 43 nucleotides
BT0965: BT0965 - putative anti-sigma factor (NCBI ptt file), at 1,182,131 to 1,183,105
BT0965
BT0966: BT0966 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 1,183,169 to 1,183,720
BT0966
BT0967: BT0967 - hypothetical protein (NCBI ptt file), at 1,183,815 to 1,184,036
BT0967
BT0968: BT0968 - conserved hypothetical protein (NCBI ptt file), at 1,184,080 to 1,187,214
BT0968
Position (kb)
1183
1184
1185 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1182.819 kb on - strand, within BT0965 at 1182.826 kb on + strand, within BT0965 at 1182.881 kb on + strand, within BT0965 at 1183.121 kb on + strand at 1183.124 kb on - strand at 1183.198 kb on + strand at 1183.198 kb on + strand at 1183.268 kb on + strand, within BT0966 at 1183.268 kb on + strand, within BT0966 at 1183.274 kb on + strand, within BT0966 at 1183.312 kb on + strand, within BT0966 at 1183.313 kb on - strand, within BT0966 at 1183.359 kb on - strand, within BT0966 at 1183.361 kb on + strand, within BT0966 at 1183.364 kb on + strand, within BT0966 at 1183.370 kb on + strand, within BT0966 at 1183.371 kb on - strand, within BT0966 at 1183.371 kb on - strand, within BT0966 at 1183.433 kb on + strand, within BT0966 at 1183.438 kb on - strand, within BT0966 at 1183.452 kb on + strand, within BT0966 at 1183.452 kb on + strand, within BT0966 at 1183.504 kb on - strand, within BT0966 at 1183.603 kb on + strand, within BT0966 at 1183.603 kb on + strand, within BT0966 at 1183.605 kb on + strand, within BT0966 at 1183.636 kb on - strand, within BT0966 at 1183.638 kb on + strand, within BT0966 at 1183.638 kb on + strand, within BT0966 at 1183.646 kb on - strand, within BT0966 at 1183.711 kb on + strand at 1183.807 kb on + strand at 1183.817 kb on - strand at 1183.838 kb on - strand, within BT0967 at 1183.899 kb on - strand, within BT0967 at 1183.959 kb on + strand, within BT0967 at 1184.139 kb on + strand at 1184.208 kb on + strand at 1184.274 kb on - strand at 1184.393 kb on + strand at 1184.532 kb on - strand, within BT0968 at 1184.565 kb on - strand, within BT0968 at 1184.576 kb on - strand, within BT0968 at 1184.609 kb on + strand, within BT0968 at 1184.728 kb on + strand, within BT0968 at 1184.738 kb on + strand, within BT0968 at 1184.787 kb on + strand, within BT0968 at 1184.792 kb on - strand, within BT0968 at 1185.033 kb on + strand, within BT0968
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 1,182,819 - BT0965 0.71 -2.1 1,182,826 + BT0965 0.71 +1.1 1,182,881 + BT0965 0.77 +0.1 1,183,121 + -0.5 1,183,124 - -0.7 1,183,198 + +0.1 1,183,198 + -0.6 1,183,268 + BT0966 0.18 +0.1 1,183,268 + BT0966 0.18 -0.8 1,183,274 + BT0966 0.19 +0.1 1,183,312 + BT0966 0.26 -0.3 1,183,313 - BT0966 0.26 +0.1 1,183,359 - BT0966 0.34 -0.9 1,183,361 + BT0966 0.35 -1.7 1,183,364 + BT0966 0.35 -2.5 1,183,370 + BT0966 0.36 -1.2 1,183,371 - BT0966 0.37 +0.1 1,183,371 - BT0966 0.37 +0.1 1,183,433 + BT0966 0.48 -1.3 1,183,438 - BT0966 0.49 -1.3 1,183,452 + BT0966 0.51 +0.4 1,183,452 + BT0966 0.51 -2.1 1,183,504 - BT0966 0.61 -0.7 1,183,603 + BT0966 0.79 -1.2 1,183,603 + BT0966 0.79 -1.3 1,183,605 + BT0966 0.79 +0.1 1,183,636 - BT0966 0.85 -2.1 1,183,638 + BT0966 0.85 +1.7 1,183,638 + BT0966 0.85 +0.7 1,183,646 - BT0966 0.86 -1.0 1,183,711 + +0.4 1,183,807 + -0.9 1,183,817 - +1.1 1,183,838 - BT0967 0.10 -0.5 1,183,899 - BT0967 0.38 -3.7 1,183,959 + BT0967 0.65 -1.3 1,184,139 + -0.9 1,184,208 + +1.1 1,184,274 - +0.5 1,184,393 + -2.3 1,184,532 - BT0968 0.14 -0.5 1,184,565 - BT0968 0.15 +1.7 1,184,576 - BT0968 0.16 -1.3 1,184,609 + BT0968 0.17 +0.6 1,184,728 + BT0968 0.21 -0.5 1,184,738 + BT0968 0.21 -1.3 1,184,787 + BT0968 0.23 -0.9 1,184,792 - BT0968 0.23 +1.1 1,185,033 + BT0968 0.30 +1.1
Or see this region's nucleotide sequence