Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0489 and BT0490 are separated by 201 nucleotides BT0490 and BT0491 are separated by 335 nucleotides BT0491 and BT0492 overlap by 4 nucleotides BT0492 and BT0493 are separated by 33 nucleotides
BT0489: BT0489 - putative gluconate aldolase (NCBI ptt file), at 605,607 to 606,278
BT0489
BT0490: BT0490 - hypothetical protein (NCBI ptt file), at 606,480 to 606,746
BT0490
BT0491: BT0491 - conserved hypothetical protein (NCBI ptt file), at 607,082 to 607,408
BT0491
BT0492: BT0492 - integral membrane protein (NCBI ptt file), at 607,405 to 608,022
BT0492
BT0493: BT0493 - hypothetical protein (NCBI ptt file), at 608,056 to 608,757
BT0493
Position (kb) 607
608 Strain fitness (log2 ratio) -2
-1
0
1 at 606.340 kb on + strand at 606.356 kb on - strand at 606.360 kb on - strand at 606.364 kb on + strand at 606.371 kb on + strand at 606.372 kb on - strand at 606.469 kb on - strand at 606.614 kb on + strand, within BT0490 at 606.663 kb on + strand, within BT0490 at 606.663 kb on + strand, within BT0490 at 606.664 kb on - strand, within BT0490 at 606.740 kb on + strand at 606.762 kb on - strand at 606.768 kb on + strand at 606.772 kb on + strand at 606.772 kb on + strand at 606.799 kb on + strand at 606.800 kb on - strand at 606.828 kb on - strand at 606.844 kb on + strand at 606.879 kb on + strand at 606.903 kb on - strand at 606.911 kb on + strand at 606.983 kb on + strand at 606.983 kb on + strand at 606.983 kb on + strand at 607.079 kb on + strand at 607.080 kb on - strand at 607.112 kb on - strand at 607.214 kb on - strand, within BT0491 at 607.289 kb on - strand, within BT0491 at 607.289 kb on - strand, within BT0491 at 607.307 kb on + strand, within BT0491 at 607.476 kb on + strand, within BT0492 at 607.578 kb on - strand, within BT0492 at 607.578 kb on - strand, within BT0492 at 607.593 kb on + strand, within BT0492 at 607.694 kb on - strand, within BT0492 at 607.780 kb on - strand, within BT0492 at 607.910 kb on + strand, within BT0492 at 607.958 kb on - strand, within BT0492 at 608.005 kb on - strand at 608.005 kb on - strand at 608.013 kb on + strand at 608.014 kb on - strand at 608.027 kb on - strand at 608.140 kb on + strand, within BT0493 at 608.288 kb on + strand, within BT0493 at 608.296 kb on + strand, within BT0493 at 608.391 kb on - strand, within BT0493
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 606,340 + -0.7 606,356 - -1.4 606,360 - -1.1 606,364 + -0.6 606,371 + -1.1 606,372 - -0.5 606,469 - +0.9 606,614 + BT0490 0.50 -0.4 606,663 + BT0490 0.69 -0.1 606,663 + BT0490 0.69 -0.1 606,664 - BT0490 0.69 -0.7 606,740 + -0.4 606,762 - -1.0 606,768 + +0.1 606,772 + -0.1 606,772 + -0.8 606,799 + -0.7 606,800 - -0.3 606,828 - -1.6 606,844 + -0.9 606,879 + -0.5 606,903 - -1.6 606,911 + +0.5 606,983 + -1.3 606,983 + -0.6 606,983 + -0.4 607,079 + +0.3 607,080 - -0.1 607,112 - -0.8 607,214 - BT0491 0.40 -0.7 607,289 - BT0491 0.63 +0.9 607,289 - BT0491 0.63 -2.7 607,307 + BT0491 0.69 -1.1 607,476 + BT0492 0.11 -1.1 607,578 - BT0492 0.28 +0.9 607,578 - BT0492 0.28 -2.5 607,593 + BT0492 0.30 +0.9 607,694 - BT0492 0.47 -1.3 607,780 - BT0492 0.61 +0.9 607,910 + BT0492 0.82 -0.3 607,958 - BT0492 0.89 -1.4 608,005 - -0.1 608,005 - +0.9 608,013 + -0.7 608,014 - +0.9 608,027 - -1.7 608,140 + BT0493 0.12 -0.9 608,288 + BT0493 0.33 -1.7 608,296 + BT0493 0.34 -1.0 608,391 - BT0493 0.48 -1.4
Or see this region's nucleotide sequence