Experiment: 4-Hydroxyacetophenone 0.00025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_67 and Pf6N2E2_68 are separated by 153 nucleotides Pf6N2E2_68 and Pf6N2E2_69 are separated by 210 nucleotides
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467
_68
Pf6N2E2_69 - Probable type IV pilus assembly FimV-related transmembrane protein, at 109,678 to 112,272
_69
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1 at 108.356 kb on - strand at 108.441 kb on + strand at 108.450 kb on + strand at 108.451 kb on - strand at 108.451 kb on - strand at 108.451 kb on - strand at 109.084 kb on - strand, within Pf6N2E2_68 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.195 kb on - strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.398 kb on + strand at 109.451 kb on + strand at 109.465 kb on + strand at 109.472 kb on + strand at 109.472 kb on + strand at 109.500 kb on + strand at 109.511 kb on + strand at 109.511 kb on + strand at 109.529 kb on + strand at 109.530 kb on - strand at 109.618 kb on - strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.678 kb on - strand at 109.703 kb on + strand at 109.703 kb on + strand at 109.704 kb on - strand at 109.704 kb on - strand at 109.709 kb on + strand at 109.710 kb on - strand at 110.051 kb on - strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyacetophenone 0.00025 M remove 108,356 - +1.2 108,441 + -0.0 108,450 + +0.5 108,451 - -0.0 108,451 - +0.9 108,451 - -0.6 109,084 - Pf6N2E2_68 0.62 -0.6 109,084 - Pf6N2E2_68 0.62 +0.1 109,195 - Pf6N2E2_68 0.73 -0.9 109,333 + Pf6N2E2_68 0.87 +0.2 109,333 + Pf6N2E2_68 0.87 -0.9 109,334 - Pf6N2E2_68 0.87 -0.7 109,334 - Pf6N2E2_68 0.87 +0.4 109,334 - Pf6N2E2_68 0.87 -1.1 109,376 + -0.3 109,376 + +0.8 109,376 + -0.7 109,376 + -0.6 109,376 + +0.8 109,377 - -0.4 109,377 - -0.3 109,377 - -0.0 109,380 + -0.2 109,380 + +0.8 109,380 + -0.4 109,381 - -2.0 109,381 - -1.4 109,381 - -2.2 109,381 - +0.8 109,398 + +0.1 109,451 + -0.1 109,465 + +0.3 109,472 + +0.8 109,472 + +0.1 109,500 + +0.7 109,511 + -0.6 109,511 + +0.8 109,529 + -2.3 109,530 - +0.5 109,618 - +0.0 109,634 + -1.8 109,634 + +0.8 109,634 + -0.6 109,634 + +1.2 109,678 - -0.7 109,703 + -0.2 109,703 + -0.3 109,704 - -0.1 109,704 - +1.4 109,709 + +1.3 109,710 - +0.3 110,051 - Pf6N2E2_69 0.14 -0.0 110,057 + Pf6N2E2_69 0.15 +0.9 110,057 + Pf6N2E2_69 0.15 +0.8 110,057 + Pf6N2E2_69 0.15 +1.1 110,058 - Pf6N2E2_69 0.15 +0.8 110,058 - Pf6N2E2_69 0.15 -0.2 110,058 - Pf6N2E2_69 0.15 +1.2 110,164 + Pf6N2E2_69 0.19 -2.1 110,164 + Pf6N2E2_69 0.19 +0.7 110,165 - Pf6N2E2_69 0.19 +0.5 110,165 - Pf6N2E2_69 0.19 -0.8 110,165 - Pf6N2E2_69 0.19 -0.4
Or see this region's nucleotide sequence