Experiment: 4-Hydroxyacetophenone 0.00025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5385 and Pf6N2E2_5386 are separated by 99 nucleotides Pf6N2E2_5386 and Pf6N2E2_5387 are separated by 35 nucleotides
Pf6N2E2_5385 - Alginate biosynthesis protein AlgK precursor, at 6,188,941 to 6,190,341
_5385
Pf6N2E2_5386 - Alginate biosynthesis protein Alg44, at 6,190,441 to 6,191,610
_5386
Pf6N2E2_5387 - Alginate biosynthesis protein Alg8, at 6,191,646 to 6,193,127
_5387
Position (kb)
6190
6191
6192 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.459 kb on - strand, within Pf6N2E2_5385 at 6189.501 kb on - strand, within Pf6N2E2_5385 at 6189.719 kb on + strand, within Pf6N2E2_5385 at 6189.720 kb on - strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.741 kb on - strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6190.176 kb on - strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.414 kb on + strand at 6190.415 kb on - strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.431 kb on + strand at 6190.432 kb on - strand at 6190.432 kb on - strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.446 kb on + strand at 6190.446 kb on + strand at 6190.511 kb on - strand at 6190.665 kb on + strand, within Pf6N2E2_5386 at 6191.004 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.067 kb on - strand, within Pf6N2E2_5386 at 6191.345 kb on + strand, within Pf6N2E2_5386 at 6191.346 kb on - strand, within Pf6N2E2_5386 at 6191.417 kb on + strand, within Pf6N2E2_5386 at 6191.418 kb on - strand, within Pf6N2E2_5386 at 6191.506 kb on - strand at 6191.584 kb on + strand at 6191.584 kb on + strand at 6191.584 kb on + strand at 6191.585 kb on - strand at 6191.585 kb on - strand at 6191.585 kb on - strand at 6191.624 kb on + strand at 6191.625 kb on - strand at 6191.628 kb on + strand at 6191.628 kb on + strand at 6191.628 kb on + strand at 6191.629 kb on - strand at 6191.629 kb on - strand at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.806 kb on + strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.807 kb on - strand, within Pf6N2E2_5387 at 6191.839 kb on + strand, within Pf6N2E2_5387 at 6191.840 kb on - strand, within Pf6N2E2_5387 at 6191.840 kb on - strand, within Pf6N2E2_5387 at 6191.842 kb on + strand, within Pf6N2E2_5387 at 6191.935 kb on + strand, within Pf6N2E2_5387 at 6191.935 kb on + strand, within Pf6N2E2_5387 at 6191.936 kb on - strand, within Pf6N2E2_5387 at 6191.936 kb on - strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.959 kb on + strand, within Pf6N2E2_5387 at 6191.960 kb on - strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.196 kb on + strand, within Pf6N2E2_5387 at 6192.197 kb on - strand, within Pf6N2E2_5387 at 6192.454 kb on + strand, within Pf6N2E2_5387 at 6192.455 kb on - strand, within Pf6N2E2_5387 at 6192.580 kb on - strand, within Pf6N2E2_5387
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyacetophenone 0.00025 M remove 6,189,458 + Pf6N2E2_5385 0.37 +0.7 6,189,458 + Pf6N2E2_5385 0.37 +0.3 6,189,459 - Pf6N2E2_5385 0.37 -0.0 6,189,501 - Pf6N2E2_5385 0.40 +1.2 6,189,719 + Pf6N2E2_5385 0.56 +0.9 6,189,720 - Pf6N2E2_5385 0.56 +0.4 6,189,740 + Pf6N2E2_5385 0.57 +0.1 6,189,740 + Pf6N2E2_5385 0.57 +0.2 6,189,740 + Pf6N2E2_5385 0.57 +0.1 6,189,741 - Pf6N2E2_5385 0.57 +1.5 6,189,806 + Pf6N2E2_5385 0.62 -1.4 6,189,806 + Pf6N2E2_5385 0.62 -0.4 6,189,807 - Pf6N2E2_5385 0.62 -2.5 6,189,807 - Pf6N2E2_5385 0.62 +0.0 6,189,807 - Pf6N2E2_5385 0.62 -0.5 6,189,807 - Pf6N2E2_5385 0.62 -0.2 6,189,929 + Pf6N2E2_5385 0.71 +0.0 6,189,929 + Pf6N2E2_5385 0.71 +0.9 6,189,929 + Pf6N2E2_5385 0.71 +0.7 6,189,930 - Pf6N2E2_5385 0.71 -0.3 6,189,930 - Pf6N2E2_5385 0.71 +0.1 6,190,176 - Pf6N2E2_5385 0.88 -0.5 6,190,199 + Pf6N2E2_5385 0.90 -0.2 6,190,199 + Pf6N2E2_5385 0.90 +0.5 6,190,199 + Pf6N2E2_5385 0.90 +1.4 6,190,200 - Pf6N2E2_5385 0.90 -0.4 6,190,200 - Pf6N2E2_5385 0.90 +0.2 6,190,200 - Pf6N2E2_5385 0.90 -0.4 6,190,200 - Pf6N2E2_5385 0.90 -0.8 6,190,200 - Pf6N2E2_5385 0.90 +0.8 6,190,200 - Pf6N2E2_5385 0.90 -0.7 6,190,200 - Pf6N2E2_5385 0.90 -0.3 6,190,200 - Pf6N2E2_5385 0.90 -0.6 6,190,341 + +0.2 6,190,341 + +0.2 6,190,341 + -0.1 6,190,341 + +0.5 6,190,341 + +0.3 6,190,342 - -1.1 6,190,342 - -0.1 6,190,342 - +0.1 6,190,414 + -2.4 6,190,415 - -0.5 6,190,416 + +0.4 6,190,416 + -1.1 6,190,416 + +0.4 6,190,431 + -0.2 6,190,432 - +0.9 6,190,432 - +0.2 6,190,442 + -0.2 6,190,442 + -2.4 6,190,442 + -0.5 6,190,442 + -0.8 6,190,442 + +2.2 6,190,442 + +0.0 6,190,446 + +0.8 6,190,446 + -1.0 6,190,511 - -0.4 6,190,665 + Pf6N2E2_5386 0.19 +0.9 6,191,004 + Pf6N2E2_5386 0.48 -0.2 6,191,057 + Pf6N2E2_5386 0.53 +0.4 6,191,057 + Pf6N2E2_5386 0.53 -0.6 6,191,057 + Pf6N2E2_5386 0.53 +0.9 6,191,057 + Pf6N2E2_5386 0.53 -1.0 6,191,057 + Pf6N2E2_5386 0.53 +0.7 6,191,057 + Pf6N2E2_5386 0.53 +0.3 6,191,057 + Pf6N2E2_5386 0.53 +0.6 6,191,057 + Pf6N2E2_5386 0.53 -0.0 6,191,057 + Pf6N2E2_5386 0.53 +1.0 6,191,058 - Pf6N2E2_5386 0.53 +0.9 6,191,058 - Pf6N2E2_5386 0.53 +0.3 6,191,058 - Pf6N2E2_5386 0.53 +0.6 6,191,058 - Pf6N2E2_5386 0.53 +1.2 6,191,058 - Pf6N2E2_5386 0.53 +1.6 6,191,058 - Pf6N2E2_5386 0.53 -0.2 6,191,067 - Pf6N2E2_5386 0.54 -0.6 6,191,345 + Pf6N2E2_5386 0.77 +0.4 6,191,346 - Pf6N2E2_5386 0.77 +0.0 6,191,417 + Pf6N2E2_5386 0.83 +0.5 6,191,418 - Pf6N2E2_5386 0.84 +0.7 6,191,506 - +0.2 6,191,584 + -0.6 6,191,584 + +1.0 6,191,584 + -0.4 6,191,585 - -2.7 6,191,585 - +0.7 6,191,585 - -0.6 6,191,624 + -0.2 6,191,625 - -0.7 6,191,628 + +1.1 6,191,628 + +2.2 6,191,628 + -1.3 6,191,629 - -0.9 6,191,629 - -1.7 6,191,806 + Pf6N2E2_5387 0.11 -0.9 6,191,806 + Pf6N2E2_5387 0.11 +0.3 6,191,806 + Pf6N2E2_5387 0.11 -0.5 6,191,806 + Pf6N2E2_5387 0.11 -0.6 6,191,806 + Pf6N2E2_5387 0.11 -1.0 6,191,806 + Pf6N2E2_5387 0.11 -1.5 6,191,806 + Pf6N2E2_5387 0.11 +0.0 6,191,806 + Pf6N2E2_5387 0.11 -1.2 6,191,806 + Pf6N2E2_5387 0.11 +0.4 6,191,806 + Pf6N2E2_5387 0.11 -0.1 6,191,807 - Pf6N2E2_5387 0.11 +0.3 6,191,807 - Pf6N2E2_5387 0.11 -1.4 6,191,807 - Pf6N2E2_5387 0.11 -2.0 6,191,807 - Pf6N2E2_5387 0.11 -0.2 6,191,839 + Pf6N2E2_5387 0.13 +2.5 6,191,840 - Pf6N2E2_5387 0.13 -2.4 6,191,840 - Pf6N2E2_5387 0.13 -0.6 6,191,842 + Pf6N2E2_5387 0.13 +0.3 6,191,935 + Pf6N2E2_5387 0.20 -0.1 6,191,935 + Pf6N2E2_5387 0.20 -0.2 6,191,936 - Pf6N2E2_5387 0.20 -1.2 6,191,936 - Pf6N2E2_5387 0.20 +0.6 6,191,959 + Pf6N2E2_5387 0.21 -0.6 6,191,959 + Pf6N2E2_5387 0.21 -0.3 6,191,959 + Pf6N2E2_5387 0.21 -0.2 6,191,960 - Pf6N2E2_5387 0.21 +0.4 6,192,196 + Pf6N2E2_5387 0.37 -0.4 6,192,196 + Pf6N2E2_5387 0.37 +0.2 6,192,196 + Pf6N2E2_5387 0.37 +0.7 6,192,196 + Pf6N2E2_5387 0.37 +0.8 6,192,196 + Pf6N2E2_5387 0.37 -0.3 6,192,196 + Pf6N2E2_5387 0.37 +0.1 6,192,196 + Pf6N2E2_5387 0.37 -1.4 6,192,196 + Pf6N2E2_5387 0.37 -2.8 6,192,196 + Pf6N2E2_5387 0.37 -0.4 6,192,196 + Pf6N2E2_5387 0.37 +0.1 6,192,196 + Pf6N2E2_5387 0.37 +0.2 6,192,196 + Pf6N2E2_5387 0.37 -0.0 6,192,197 - Pf6N2E2_5387 0.37 -0.0 6,192,454 + Pf6N2E2_5387 0.55 -0.5 6,192,455 - Pf6N2E2_5387 0.55 -0.2 6,192,580 - Pf6N2E2_5387 0.63 -0.8
Or see this region's nucleotide sequence