Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00840 and PGA1_c00850 are separated by 326 nucleotides PGA1_c00850 and PGA1_c00860 are separated by 156 nucleotides PGA1_c00860 and PGA1_c00870 are separated by 97 nucleotides PGA1_c00870 and PGA1_c00880 are separated by 75 nucleotides
PGA1_c00840: PGA1_c00840 - glutathione transferase-like protein, at 83,063 to 84,037
_c00840
PGA1_c00850: PGA1_c00850 - hypothetical protein, at 84,364 to 84,726
_c00850
PGA1_c00860: PGA1_c00860 - cystathionine beta-lyase PatB, at 84,883 to 86,055
_c00860
PGA1_c00870: PGA1_c00870 - peptide deformylase Def, at 86,153 to 86,671
_c00870
PGA1_c00880: PGA1_c00880 - peptide deformylase Def, at 86,747 to 87,256
_c00880
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 83.908 kb on - strand, within PGA1_c00840 at 83.913 kb on + strand, within PGA1_c00840 at 84.044 kb on + strand at 84.046 kb on + strand at 84.052 kb on - strand at 84.052 kb on - strand at 84.054 kb on - strand at 84.054 kb on - strand at 84.064 kb on - strand at 84.076 kb on - strand at 84.084 kb on - strand at 84.084 kb on - strand at 84.088 kb on - strand at 84.105 kb on - strand at 84.139 kb on + strand at 84.146 kb on + strand at 84.146 kb on + strand at 84.146 kb on + strand at 84.154 kb on - strand at 84.161 kb on + strand at 84.185 kb on + strand at 84.190 kb on + strand at 84.194 kb on + strand at 84.202 kb on + strand at 84.202 kb on + strand at 84.220 kb on - strand at 84.263 kb on + strand at 84.274 kb on + strand at 84.279 kb on - strand at 84.279 kb on - strand at 84.282 kb on - strand at 84.282 kb on - strand at 84.282 kb on - strand at 84.325 kb on + strand at 84.366 kb on + strand at 84.401 kb on - strand, within PGA1_c00850 at 84.412 kb on + strand, within PGA1_c00850 at 84.414 kb on + strand, within PGA1_c00850 at 84.414 kb on + strand, within PGA1_c00850 at 84.415 kb on - strand, within PGA1_c00850 at 84.415 kb on - strand, within PGA1_c00850 at 84.422 kb on - strand, within PGA1_c00850 at 84.422 kb on - strand, within PGA1_c00850 at 84.521 kb on + strand, within PGA1_c00850 at 84.540 kb on + strand, within PGA1_c00850 at 84.607 kb on + strand, within PGA1_c00850 at 84.607 kb on + strand, within PGA1_c00850 at 84.677 kb on + strand, within PGA1_c00850 at 84.687 kb on - strand, within PGA1_c00850 at 84.689 kb on + strand, within PGA1_c00850 at 84.689 kb on + strand, within PGA1_c00850 at 84.699 kb on + strand at 84.728 kb on - strand at 84.736 kb on + strand at 84.739 kb on - strand at 84.756 kb on - strand at 84.765 kb on - strand at 84.856 kb on - strand at 84.864 kb on + strand at 84.864 kb on + strand at 84.872 kb on - strand at 84.872 kb on - strand at 84.872 kb on - strand at 84.882 kb on + strand at 84.926 kb on + strand at 84.926 kb on + strand at 84.927 kb on + strand at 84.934 kb on - strand at 84.934 kb on - strand at 85.026 kb on + strand, within PGA1_c00860 at 85.026 kb on + strand, within PGA1_c00860 at 85.026 kb on + strand, within PGA1_c00860 at 85.026 kb on + strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.034 kb on - strand, within PGA1_c00860 at 85.054 kb on + strand, within PGA1_c00860 at 85.054 kb on + strand, within PGA1_c00860 at 85.054 kb on + strand, within PGA1_c00860 at 85.062 kb on - strand, within PGA1_c00860 at 85.062 kb on - strand, within PGA1_c00860 at 85.062 kb on - strand, within PGA1_c00860 at 85.062 kb on - strand, within PGA1_c00860 at 85.062 kb on - strand, within PGA1_c00860 at 85.091 kb on - strand, within PGA1_c00860 at 85.098 kb on + strand, within PGA1_c00860 at 85.102 kb on - strand, within PGA1_c00860 at 85.104 kb on + strand, within PGA1_c00860 at 85.106 kb on + strand, within PGA1_c00860 at 85.112 kb on - strand, within PGA1_c00860 at 85.112 kb on - strand, within PGA1_c00860 at 85.122 kb on - strand, within PGA1_c00860 at 85.122 kb on - strand, within PGA1_c00860 at 85.125 kb on + strand, within PGA1_c00860 at 85.129 kb on - strand, within PGA1_c00860 at 85.145 kb on + strand, within PGA1_c00860 at 85.183 kb on - strand, within PGA1_c00860 at 85.203 kb on + strand, within PGA1_c00860 at 85.210 kb on - strand, within PGA1_c00860 at 85.315 kb on + strand, within PGA1_c00860 at 85.318 kb on - strand, within PGA1_c00860 at 85.390 kb on + strand, within PGA1_c00860 at 85.395 kb on + strand, within PGA1_c00860 at 85.398 kb on - strand, within PGA1_c00860 at 85.398 kb on - strand, within PGA1_c00860 at 85.413 kb on + strand, within PGA1_c00860 at 85.413 kb on + strand, within PGA1_c00860 at 85.413 kb on + strand, within PGA1_c00860 at 85.436 kb on + strand, within PGA1_c00860 at 85.476 kb on - strand, within PGA1_c00860 at 85.509 kb on + strand, within PGA1_c00860 at 85.509 kb on + strand, within PGA1_c00860 at 85.509 kb on + strand, within PGA1_c00860 at 85.520 kb on + strand, within PGA1_c00860 at 85.520 kb on + strand, within PGA1_c00860 at 85.520 kb on + strand, within PGA1_c00860 at 85.520 kb on + strand, within PGA1_c00860 at 85.528 kb on - strand, within PGA1_c00860 at 85.557 kb on + strand, within PGA1_c00860 at 85.651 kb on - strand, within PGA1_c00860 at 85.653 kb on + strand, within PGA1_c00860 at 85.661 kb on - strand, within PGA1_c00860 at 85.661 kb on - strand, within PGA1_c00860 at 85.661 kb on - strand, within PGA1_c00860 at 85.680 kb on + strand, within PGA1_c00860 at 85.700 kb on + strand, within PGA1_c00860 at 85.700 kb on + strand, within PGA1_c00860 at 85.700 kb on + strand, within PGA1_c00860 at 85.700 kb on + strand, within PGA1_c00860 at 85.708 kb on - strand, within PGA1_c00860 at 85.739 kb on - strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.760 kb on + strand, within PGA1_c00860 at 85.768 kb on - strand, within PGA1_c00860 at 85.768 kb on - strand, within PGA1_c00860 at 85.770 kb on + strand, within PGA1_c00860 at 85.770 kb on + strand, within PGA1_c00860 at 85.770 kb on + strand, within PGA1_c00860 at 85.773 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.778 kb on - strand, within PGA1_c00860 at 85.783 kb on - strand, within PGA1_c00860 at 85.829 kb on + strand, within PGA1_c00860 at 85.846 kb on + strand, within PGA1_c00860 at 85.864 kb on + strand, within PGA1_c00860 at 85.873 kb on + strand, within PGA1_c00860 at 85.890 kb on - strand, within PGA1_c00860 at 85.913 kb on + strand, within PGA1_c00860 at 85.925 kb on - strand, within PGA1_c00860 at 85.925 kb on - strand, within PGA1_c00860 at 85.925 kb on - strand, within PGA1_c00860 at 86.000 kb on - strand at 86.033 kb on + strand at 86.063 kb on - strand at 86.071 kb on + strand at 86.123 kb on - strand at 86.628 kb on + strand at 86.696 kb on - strand at 86.705 kb on + strand at 86.711 kb on + strand at 86.711 kb on + strand at 86.711 kb on + strand at 86.711 kb on + strand at 86.713 kb on - strand at 86.720 kb on - strand at 86.791 kb on - strand at 86.889 kb on + strand, within PGA1_c00880 at 86.934 kb on + strand, within PGA1_c00880 at 86.942 kb on - strand, within PGA1_c00880 at 86.957 kb on + strand, within PGA1_c00880 at 86.957 kb on + strand, within PGA1_c00880 at 86.962 kb on + strand, within PGA1_c00880 at 86.965 kb on - strand, within PGA1_c00880 at 86.982 kb on + strand, within PGA1_c00880 at 87.045 kb on + strand, within PGA1_c00880 at 87.045 kb on + strand, within PGA1_c00880 at 87.053 kb on - strand, within PGA1_c00880 at 87.053 kb on - strand, within PGA1_c00880
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 83,908 - PGA1_c00840 0.87 -1.0 83,913 + PGA1_c00840 0.87 +0.3 84,044 + +0.6 84,046 + -0.1 84,052 - -0.4 84,052 - +1.0 84,054 - +0.0 84,054 - +0.5 84,064 - -0.5 84,076 - -0.5 84,084 - -0.4 84,084 - -0.4 84,088 - +0.3 84,105 - -0.5 84,139 + -0.4 84,146 + +1.0 84,146 + +1.6 84,146 + +0.3 84,154 - +0.4 84,161 + +0.8 84,185 + -0.5 84,190 + -1.2 84,194 + +1.1 84,202 + +0.4 84,202 + -0.6 84,220 - +0.1 84,263 + +0.3 84,274 + -0.3 84,279 - -0.8 84,279 - +1.5 84,282 - -1.4 84,282 - +0.1 84,282 - -0.3 84,325 + -1.8 84,366 + -0.3 84,401 - PGA1_c00850 0.10 +0.4 84,412 + PGA1_c00850 0.13 +0.1 84,414 + PGA1_c00850 0.14 -0.4 84,414 + PGA1_c00850 0.14 -0.1 84,415 - PGA1_c00850 0.14 -0.9 84,415 - PGA1_c00850 0.14 +1.2 84,422 - PGA1_c00850 0.16 +0.5 84,422 - PGA1_c00850 0.16 -0.7 84,521 + PGA1_c00850 0.43 -0.1 84,540 + PGA1_c00850 0.48 -1.3 84,607 + PGA1_c00850 0.67 -0.7 84,607 + PGA1_c00850 0.67 +0.5 84,677 + PGA1_c00850 0.86 +1.3 84,687 - PGA1_c00850 0.89 +0.6 84,689 + PGA1_c00850 0.90 +1.2 84,689 + PGA1_c00850 0.90 +0.3 84,699 + -0.8 84,728 - -1.0 84,736 + +0.8 84,739 - +1.0 84,756 - -0.5 84,765 - -0.2 84,856 - -0.5 84,864 + +0.8 84,864 + +0.4 84,872 - -1.1 84,872 - +1.3 84,872 - +2.8 84,882 + +1.2 84,926 + +0.5 84,926 + -2.3 84,927 + +0.2 84,934 - -2.3 84,934 - -1.4 85,026 + PGA1_c00860 0.12 -0.0 85,026 + PGA1_c00860 0.12 -0.7 85,026 + PGA1_c00860 0.12 +0.4 85,026 + PGA1_c00860 0.12 -1.2 85,034 - PGA1_c00860 0.13 +0.1 85,034 - PGA1_c00860 0.13 -0.4 85,034 - PGA1_c00860 0.13 -0.4 85,034 - PGA1_c00860 0.13 +0.4 85,034 - PGA1_c00860 0.13 -1.5 85,034 - PGA1_c00860 0.13 -2.4 85,034 - PGA1_c00860 0.13 -0.8 85,034 - PGA1_c00860 0.13 -0.4 85,034 - PGA1_c00860 0.13 -1.0 85,034 - PGA1_c00860 0.13 -0.7 85,054 + PGA1_c00860 0.15 -0.3 85,054 + PGA1_c00860 0.15 -1.8 85,054 + PGA1_c00860 0.15 -0.4 85,062 - PGA1_c00860 0.15 -2.1 85,062 - PGA1_c00860 0.15 -2.5 85,062 - PGA1_c00860 0.15 -3.7 85,062 - PGA1_c00860 0.15 -1.1 85,062 - PGA1_c00860 0.15 -1.1 85,091 - PGA1_c00860 0.18 +0.1 85,098 + PGA1_c00860 0.18 -1.9 85,102 - PGA1_c00860 0.19 -0.8 85,104 + PGA1_c00860 0.19 -1.6 85,106 + PGA1_c00860 0.19 +0.4 85,112 - PGA1_c00860 0.20 -1.2 85,112 - PGA1_c00860 0.20 -1.6 85,122 - PGA1_c00860 0.20 -0.3 85,122 - PGA1_c00860 0.20 -2.2 85,125 + PGA1_c00860 0.21 +0.2 85,129 - PGA1_c00860 0.21 +0.0 85,145 + PGA1_c00860 0.22 -1.1 85,183 - PGA1_c00860 0.26 -0.5 85,203 + PGA1_c00860 0.27 -1.6 85,210 - PGA1_c00860 0.28 -2.1 85,315 + PGA1_c00860 0.37 -1.7 85,318 - PGA1_c00860 0.37 -1.8 85,390 + PGA1_c00860 0.43 -2.2 85,395 + PGA1_c00860 0.44 -1.6 85,398 - PGA1_c00860 0.44 -2.2 85,398 - PGA1_c00860 0.44 -2.1 85,413 + PGA1_c00860 0.45 -1.0 85,413 + PGA1_c00860 0.45 -0.8 85,413 + PGA1_c00860 0.45 -0.7 85,436 + PGA1_c00860 0.47 -0.5 85,476 - PGA1_c00860 0.51 -0.9 85,509 + PGA1_c00860 0.53 -1.3 85,509 + PGA1_c00860 0.53 +1.4 85,509 + PGA1_c00860 0.53 -0.6 85,520 + PGA1_c00860 0.54 -1.2 85,520 + PGA1_c00860 0.54 -2.7 85,520 + PGA1_c00860 0.54 -0.8 85,520 + PGA1_c00860 0.54 +0.5 85,528 - PGA1_c00860 0.55 -0.7 85,557 + PGA1_c00860 0.57 +0.2 85,651 - PGA1_c00860 0.65 -1.2 85,653 + PGA1_c00860 0.66 +0.3 85,661 - PGA1_c00860 0.66 +1.4 85,661 - PGA1_c00860 0.66 -1.0 85,661 - PGA1_c00860 0.66 -1.5 85,680 + PGA1_c00860 0.68 -1.7 85,700 + PGA1_c00860 0.70 -0.5 85,700 + PGA1_c00860 0.70 +0.2 85,700 + PGA1_c00860 0.70 -1.4 85,700 + PGA1_c00860 0.70 -0.7 85,708 - PGA1_c00860 0.70 -2.2 85,739 - PGA1_c00860 0.73 -1.0 85,760 + PGA1_c00860 0.75 -1.1 85,760 + PGA1_c00860 0.75 +0.5 85,760 + PGA1_c00860 0.75 -2.2 85,760 + PGA1_c00860 0.75 -2.1 85,760 + PGA1_c00860 0.75 +0.1 85,760 + PGA1_c00860 0.75 -1.3 85,760 + PGA1_c00860 0.75 -1.2 85,768 - PGA1_c00860 0.75 +0.4 85,768 - PGA1_c00860 0.75 -1.5 85,770 + PGA1_c00860 0.76 -1.0 85,770 + PGA1_c00860 0.76 +0.2 85,770 + PGA1_c00860 0.76 -1.8 85,773 - PGA1_c00860 0.76 -1.8 85,778 - PGA1_c00860 0.76 -1.0 85,778 - PGA1_c00860 0.76 -1.1 85,778 - PGA1_c00860 0.76 -1.7 85,778 - PGA1_c00860 0.76 -1.5 85,778 - PGA1_c00860 0.76 -0.8 85,778 - PGA1_c00860 0.76 +0.8 85,778 - PGA1_c00860 0.76 -0.6 85,783 - PGA1_c00860 0.77 -0.6 85,829 + PGA1_c00860 0.81 -0.5 85,846 + PGA1_c00860 0.82 -0.7 85,864 + PGA1_c00860 0.84 -1.0 85,873 + PGA1_c00860 0.84 +1.1 85,890 - PGA1_c00860 0.86 -1.4 85,913 + PGA1_c00860 0.88 -2.2 85,925 - PGA1_c00860 0.89 -1.0 85,925 - PGA1_c00860 0.89 +0.1 85,925 - PGA1_c00860 0.89 +1.4 86,000 - -1.3 86,033 + +0.1 86,063 - +0.9 86,071 + +0.3 86,123 - -1.1 86,628 + +0.2 86,696 - +0.8 86,705 + -0.0 86,711 + +2.4 86,711 + -0.7 86,711 + -0.5 86,711 + -0.8 86,713 - +0.4 86,720 - -0.3 86,791 - +1.8 86,889 + PGA1_c00880 0.28 -0.8 86,934 + PGA1_c00880 0.37 +0.3 86,942 - PGA1_c00880 0.38 +0.8 86,957 + PGA1_c00880 0.41 -0.0 86,957 + PGA1_c00880 0.41 -1.4 86,962 + PGA1_c00880 0.42 -0.1 86,965 - PGA1_c00880 0.43 -0.4 86,982 + PGA1_c00880 0.46 +0.4 87,045 + PGA1_c00880 0.58 +1.0 87,045 + PGA1_c00880 0.58 +0.4 87,053 - PGA1_c00880 0.60 +0.4 87,053 - PGA1_c00880 0.60 +0.1
Or see this region's nucleotide sequence