Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06440 and PGA1_c06450 are separated by 13 nucleotides PGA1_c06450 and PGA1_c06460 are separated by 118 nucleotides PGA1_c06460 and PGA1_c06470 are separated by 258 nucleotides
PGA1_c06440: PGA1_c06440 - Uncharacterized protein conserved in bacteria, at 658,926 to 659,456
_c06440
PGA1_c06450: PGA1_c06450 - short-chain dehydrogenases/reductase domain-containing protein, at 659,470 to 660,111
_c06450
PGA1_c06460: PGA1_c06460 - Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain, at 660,230 to 662,008
_c06460
PGA1_c06470: PGA1_c06470 - glycyl-tRNA synthetase alpha subunit, at 662,267 to 663,196
_c06470
Position (kb)
660
661
662
663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 659.239 kb on + strand, within PGA1_c06440 at 659.259 kb on + strand, within PGA1_c06440 at 659.273 kb on + strand, within PGA1_c06440 at 659.274 kb on + strand, within PGA1_c06440 at 659.284 kb on + strand, within PGA1_c06440 at 659.312 kb on - strand, within PGA1_c06440 at 659.369 kb on + strand, within PGA1_c06440 at 659.384 kb on + strand, within PGA1_c06440 at 659.415 kb on + strand at 659.420 kb on + strand at 659.437 kb on - strand at 659.534 kb on - strand at 659.550 kb on + strand, within PGA1_c06450 at 659.566 kb on - strand, within PGA1_c06450 at 659.581 kb on - strand, within PGA1_c06450 at 659.639 kb on - strand, within PGA1_c06450 at 659.711 kb on - strand, within PGA1_c06450 at 659.713 kb on + strand, within PGA1_c06450 at 659.713 kb on + strand, within PGA1_c06450 at 659.721 kb on - strand, within PGA1_c06450 at 659.721 kb on - strand, within PGA1_c06450 at 659.726 kb on - strand, within PGA1_c06450 at 659.726 kb on - strand, within PGA1_c06450 at 659.730 kb on + strand, within PGA1_c06450 at 659.795 kb on - strand, within PGA1_c06450 at 659.798 kb on + strand, within PGA1_c06450 at 659.839 kb on + strand, within PGA1_c06450 at 659.864 kb on + strand, within PGA1_c06450 at 659.870 kb on - strand, within PGA1_c06450 at 659.887 kb on - strand, within PGA1_c06450 at 660.028 kb on + strand, within PGA1_c06450 at 660.059 kb on - strand at 660.059 kb on - strand at 660.092 kb on + strand at 660.141 kb on + strand at 660.155 kb on + strand at 660.187 kb on + strand at 660.223 kb on + strand at 660.269 kb on - strand at 660.271 kb on + strand at 660.283 kb on - strand at 660.327 kb on - strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.339 kb on - strand at 660.349 kb on - strand at 660.349 kb on - strand at 660.349 kb on - strand at 660.360 kb on + strand at 660.387 kb on + strand at 660.387 kb on + strand at 660.387 kb on + strand at 660.431 kb on + strand, within PGA1_c06460 at 660.457 kb on + strand, within PGA1_c06460 at 660.465 kb on - strand, within PGA1_c06460 at 660.567 kb on - strand, within PGA1_c06460 at 660.620 kb on - strand, within PGA1_c06460 at 660.672 kb on + strand, within PGA1_c06460 at 660.673 kb on - strand, within PGA1_c06460 at 660.673 kb on - strand, within PGA1_c06460 at 660.777 kb on - strand, within PGA1_c06460 at 660.777 kb on - strand, within PGA1_c06460 at 660.791 kb on + strand, within PGA1_c06460 at 660.791 kb on + strand, within PGA1_c06460 at 660.841 kb on + strand, within PGA1_c06460 at 660.849 kb on - strand, within PGA1_c06460 at 660.849 kb on - strand, within PGA1_c06460 at 660.857 kb on - strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.975 kb on + strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.983 kb on - strand, within PGA1_c06460 at 660.993 kb on - strand, within PGA1_c06460 at 661.017 kb on - strand, within PGA1_c06460 at 661.034 kb on - strand, within PGA1_c06460 at 661.054 kb on + strand, within PGA1_c06460 at 661.169 kb on + strand, within PGA1_c06460 at 661.188 kb on - strand, within PGA1_c06460 at 661.203 kb on + strand, within PGA1_c06460 at 661.308 kb on + strand, within PGA1_c06460 at 661.336 kb on - strand, within PGA1_c06460 at 661.349 kb on + strand, within PGA1_c06460 at 661.357 kb on - strand, within PGA1_c06460 at 661.361 kb on + strand, within PGA1_c06460 at 661.362 kb on - strand, within PGA1_c06460 at 661.411 kb on - strand, within PGA1_c06460 at 661.492 kb on - strand, within PGA1_c06460 at 661.492 kb on - strand, within PGA1_c06460 at 661.562 kb on + strand, within PGA1_c06460 at 661.661 kb on + strand, within PGA1_c06460 at 661.865 kb on + strand at 661.873 kb on - strand at 661.965 kb on + strand at 662.019 kb on + strand at 662.027 kb on - strand at 662.037 kb on - strand at 662.073 kb on + strand at 662.086 kb on - strand at 662.132 kb on + strand at 662.164 kb on + strand at 662.172 kb on - strand at 662.172 kb on - strand at 662.172 kb on - strand at 662.172 kb on - strand at 662.172 kb on - strand at 662.180 kb on + strand at 662.192 kb on + strand at 662.200 kb on + strand at 662.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 659,239 + PGA1_c06440 0.59 -1.4 659,259 + PGA1_c06440 0.63 +0.6 659,273 + PGA1_c06440 0.65 +0.5 659,274 + PGA1_c06440 0.66 +0.1 659,284 + PGA1_c06440 0.67 -0.2 659,312 - PGA1_c06440 0.73 -0.5 659,369 + PGA1_c06440 0.83 -1.4 659,384 + PGA1_c06440 0.86 -0.1 659,415 + -1.2 659,420 + -0.2 659,437 - +0.0 659,534 - +0.6 659,550 + PGA1_c06450 0.12 -1.2 659,566 - PGA1_c06450 0.15 -0.8 659,581 - PGA1_c06450 0.17 -0.2 659,639 - PGA1_c06450 0.26 +1.5 659,711 - PGA1_c06450 0.38 +0.3 659,713 + PGA1_c06450 0.38 -0.1 659,713 + PGA1_c06450 0.38 -1.0 659,721 - PGA1_c06450 0.39 +0.2 659,721 - PGA1_c06450 0.39 +0.0 659,726 - PGA1_c06450 0.40 +0.1 659,726 - PGA1_c06450 0.40 -0.5 659,730 + PGA1_c06450 0.40 -1.3 659,795 - PGA1_c06450 0.51 +1.9 659,798 + PGA1_c06450 0.51 -2.5 659,839 + PGA1_c06450 0.57 +0.6 659,864 + PGA1_c06450 0.61 -2.2 659,870 - PGA1_c06450 0.62 -0.3 659,887 - PGA1_c06450 0.65 +0.0 660,028 + PGA1_c06450 0.87 +0.3 660,059 - +0.2 660,059 - -0.1 660,092 + -0.1 660,141 + +0.5 660,155 + +0.6 660,187 + +1.2 660,223 + -0.2 660,269 - -0.3 660,271 + -0.5 660,283 - -0.4 660,327 - -2.0 660,331 + -1.0 660,331 + -1.1 660,331 + -0.9 660,331 + -1.4 660,339 - -1.0 660,349 - +0.4 660,349 - +1.4 660,349 - +0.3 660,360 + +0.1 660,387 + -1.0 660,387 + +0.4 660,387 + -1.8 660,431 + PGA1_c06460 0.11 -1.4 660,457 + PGA1_c06460 0.13 +0.0 660,465 - PGA1_c06460 0.13 -0.6 660,567 - PGA1_c06460 0.19 -0.5 660,620 - PGA1_c06460 0.22 -1.0 660,672 + PGA1_c06460 0.25 -1.2 660,673 - PGA1_c06460 0.25 -1.0 660,673 - PGA1_c06460 0.25 -0.8 660,777 - PGA1_c06460 0.31 +0.1 660,777 - PGA1_c06460 0.31 -0.1 660,791 + PGA1_c06460 0.32 +0.3 660,791 + PGA1_c06460 0.32 -1.8 660,841 + PGA1_c06460 0.34 -1.0 660,849 - PGA1_c06460 0.35 +0.0 660,849 - PGA1_c06460 0.35 -0.6 660,857 - PGA1_c06460 0.35 -1.5 660,975 + PGA1_c06460 0.42 -2.5 660,975 + PGA1_c06460 0.42 -0.7 660,975 + PGA1_c06460 0.42 +0.6 660,975 + PGA1_c06460 0.42 -0.4 660,975 + PGA1_c06460 0.42 -0.7 660,975 + PGA1_c06460 0.42 -1.8 660,975 + PGA1_c06460 0.42 -0.4 660,975 + PGA1_c06460 0.42 -1.7 660,975 + PGA1_c06460 0.42 -2.6 660,975 + PGA1_c06460 0.42 -0.1 660,983 - PGA1_c06460 0.42 -0.8 660,983 - PGA1_c06460 0.42 -0.0 660,983 - PGA1_c06460 0.42 -0.4 660,983 - PGA1_c06460 0.42 -0.7 660,983 - PGA1_c06460 0.42 -1.1 660,983 - PGA1_c06460 0.42 -1.4 660,983 - PGA1_c06460 0.42 -1.5 660,983 - PGA1_c06460 0.42 -0.3 660,983 - PGA1_c06460 0.42 +0.4 660,993 - PGA1_c06460 0.43 -1.5 661,017 - PGA1_c06460 0.44 -2.2 661,034 - PGA1_c06460 0.45 -1.3 661,054 + PGA1_c06460 0.46 -0.7 661,169 + PGA1_c06460 0.53 +2.6 661,188 - PGA1_c06460 0.54 -0.8 661,203 + PGA1_c06460 0.55 -0.1 661,308 + PGA1_c06460 0.61 -0.4 661,336 - PGA1_c06460 0.62 -0.9 661,349 + PGA1_c06460 0.63 -1.2 661,357 - PGA1_c06460 0.63 -1.2 661,361 + PGA1_c06460 0.64 -1.8 661,362 - PGA1_c06460 0.64 -1.2 661,411 - PGA1_c06460 0.66 -0.6 661,492 - PGA1_c06460 0.71 +0.6 661,492 - PGA1_c06460 0.71 -0.0 661,562 + PGA1_c06460 0.75 -0.4 661,661 + PGA1_c06460 0.80 +0.0 661,865 + -0.7 661,873 - -0.1 661,965 + -0.3 662,019 + +0.2 662,027 - +0.6 662,037 - -0.2 662,073 + -1.7 662,086 - +0.4 662,132 + +0.0 662,164 + +1.4 662,172 - +1.0 662,172 - +0.3 662,172 - +0.2 662,172 - -0.4 662,172 - +0.0 662,180 + -0.1 662,192 + -0.5 662,200 + -0.4 662,200 - +0.1
Or see this region's nucleotide sequence