Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20910 and PGA1_c20920 are separated by 272 nucleotides PGA1_c20920 and PGA1_c20930 are separated by 132 nucleotides PGA1_c20930 and PGA1_c20940 are separated by 145 nucleotides
PGA1_c20910: PGA1_c20910 - PHA synthesis repressor protein PhaR, at 2,168,403 to 2,168,963
_c20910
PGA1_c20920: PGA1_c20920 - granule associated protein (phasin), at 2,169,236 to 2,169,679
_c20920
PGA1_c20930: PGA1_c20930 - poly(3-hydroxyalkanoate) polymerase PhaC, at 2,169,812 to 2,171,641
_c20930
PGA1_c20940: PGA1_c20940 - PHA depolymerase PhaZ, at 2,171,787 to 2,173,061
_c20940
Position (kb)
2169
2170
2171
2172 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2168.910 kb on - strand at 2168.910 kb on - strand at 2168.910 kb on - strand at 2168.944 kb on + strand at 2168.952 kb on - strand at 2168.952 kb on - strand at 2168.973 kb on + strand at 2168.978 kb on + strand at 2168.986 kb on - strand at 2168.999 kb on + strand at 2168.999 kb on + strand at 2169.007 kb on - strand at 2169.019 kb on + strand at 2169.027 kb on + strand at 2169.027 kb on + strand at 2169.027 kb on + strand at 2169.027 kb on + strand at 2169.035 kb on - strand at 2169.035 kb on - strand at 2169.035 kb on - strand at 2169.081 kb on - strand at 2169.102 kb on - strand at 2169.114 kb on + strand at 2169.122 kb on - strand at 2169.122 kb on - strand at 2169.156 kb on + strand at 2169.156 kb on + strand at 2169.164 kb on + strand at 2169.167 kb on - strand at 2169.167 kb on - strand at 2169.167 kb on - strand at 2169.212 kb on - strand at 2169.325 kb on - strand, within PGA1_c20920 at 2169.335 kb on - strand, within PGA1_c20920 at 2169.457 kb on - strand, within PGA1_c20920 at 2169.541 kb on - strand, within PGA1_c20920 at 2169.541 kb on - strand, within PGA1_c20920 at 2169.545 kb on + strand, within PGA1_c20920 at 2169.553 kb on - strand, within PGA1_c20920 at 2169.698 kb on - strand at 2169.962 kb on - strand at 2169.982 kb on + strand at 2170.010 kb on - strand, within PGA1_c20930 at 2170.017 kb on + strand, within PGA1_c20930 at 2170.022 kb on + strand, within PGA1_c20930 at 2170.025 kb on - strand, within PGA1_c20930 at 2170.090 kb on + strand, within PGA1_c20930 at 2170.098 kb on + strand, within PGA1_c20930 at 2170.172 kb on + strand, within PGA1_c20930 at 2170.192 kb on + strand, within PGA1_c20930 at 2170.207 kb on + strand, within PGA1_c20930 at 2170.215 kb on - strand, within PGA1_c20930 at 2170.261 kb on + strand, within PGA1_c20930 at 2170.261 kb on + strand, within PGA1_c20930 at 2170.261 kb on + strand, within PGA1_c20930 at 2170.269 kb on - strand, within PGA1_c20930 at 2170.269 kb on - strand, within PGA1_c20930 at 2170.303 kb on + strand, within PGA1_c20930 at 2170.333 kb on + strand, within PGA1_c20930 at 2170.333 kb on + strand, within PGA1_c20930 at 2170.333 kb on + strand, within PGA1_c20930 at 2170.341 kb on - strand, within PGA1_c20930 at 2170.342 kb on - strand, within PGA1_c20930 at 2170.348 kb on - strand, within PGA1_c20930 at 2170.360 kb on + strand, within PGA1_c20930 at 2170.368 kb on - strand, within PGA1_c20930 at 2170.375 kb on + strand, within PGA1_c20930 at 2170.383 kb on + strand, within PGA1_c20930 at 2170.391 kb on - strand, within PGA1_c20930 at 2170.413 kb on + strand, within PGA1_c20930 at 2170.437 kb on + strand, within PGA1_c20930 at 2170.603 kb on + strand, within PGA1_c20930 at 2170.611 kb on - strand, within PGA1_c20930 at 2170.638 kb on + strand, within PGA1_c20930 at 2170.638 kb on + strand, within PGA1_c20930 at 2170.638 kb on + strand, within PGA1_c20930 at 2170.638 kb on + strand, within PGA1_c20930 at 2170.646 kb on - strand, within PGA1_c20930 at 2170.646 kb on - strand, within PGA1_c20930 at 2170.646 kb on - strand, within PGA1_c20930 at 2170.646 kb on - strand, within PGA1_c20930 at 2170.646 kb on - strand, within PGA1_c20930 at 2170.707 kb on - strand, within PGA1_c20930 at 2170.712 kb on - strand, within PGA1_c20930 at 2170.726 kb on - strand, within PGA1_c20930 at 2170.740 kb on - strand, within PGA1_c20930 at 2170.743 kb on + strand, within PGA1_c20930 at 2170.752 kb on - strand, within PGA1_c20930 at 2170.759 kb on + strand, within PGA1_c20930 at 2170.768 kb on + strand, within PGA1_c20930 at 2170.768 kb on + strand, within PGA1_c20930 at 2170.770 kb on + strand, within PGA1_c20930 at 2170.770 kb on + strand, within PGA1_c20930 at 2170.770 kb on + strand, within PGA1_c20930 at 2170.776 kb on - strand, within PGA1_c20930 at 2170.776 kb on - strand, within PGA1_c20930 at 2170.776 kb on - strand, within PGA1_c20930 at 2170.777 kb on - strand, within PGA1_c20930 at 2170.778 kb on - strand, within PGA1_c20930 at 2170.783 kb on - strand, within PGA1_c20930 at 2170.805 kb on + strand, within PGA1_c20930 at 2170.805 kb on + strand, within PGA1_c20930 at 2170.805 kb on + strand, within PGA1_c20930 at 2170.813 kb on - strand, within PGA1_c20930 at 2170.813 kb on - strand, within PGA1_c20930 at 2170.813 kb on - strand, within PGA1_c20930 at 2170.820 kb on - strand, within PGA1_c20930 at 2170.881 kb on - strand, within PGA1_c20930 at 2170.898 kb on + strand, within PGA1_c20930 at 2170.912 kb on - strand, within PGA1_c20930 at 2170.939 kb on + strand, within PGA1_c20930 at 2170.939 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.049 kb on + strand, within PGA1_c20930 at 2171.057 kb on - strand, within PGA1_c20930 at 2171.057 kb on - strand, within PGA1_c20930 at 2171.057 kb on - strand, within PGA1_c20930 at 2171.057 kb on - strand, within PGA1_c20930 at 2171.057 kb on - strand, within PGA1_c20930 at 2171.067 kb on - strand, within PGA1_c20930 at 2171.082 kb on + strand, within PGA1_c20930 at 2171.220 kb on + strand, within PGA1_c20930 at 2171.226 kb on + strand, within PGA1_c20930 at 2171.226 kb on + strand, within PGA1_c20930 at 2171.234 kb on - strand, within PGA1_c20930 at 2171.240 kb on - strand, within PGA1_c20930 at 2171.243 kb on - strand, within PGA1_c20930 at 2171.302 kb on + strand, within PGA1_c20930 at 2171.310 kb on - strand, within PGA1_c20930 at 2171.324 kb on + strand, within PGA1_c20930 at 2171.324 kb on + strand, within PGA1_c20930 at 2171.326 kb on + strand, within PGA1_c20930 at 2171.326 kb on + strand, within PGA1_c20930 at 2171.326 kb on + strand, within PGA1_c20930 at 2171.326 kb on + strand, within PGA1_c20930 at 2171.326 kb on + strand, within PGA1_c20930 at 2171.332 kb on - strand, within PGA1_c20930 at 2171.332 kb on - strand, within PGA1_c20930 at 2171.332 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.334 kb on - strand, within PGA1_c20930 at 2171.390 kb on + strand, within PGA1_c20930 at 2171.392 kb on + strand, within PGA1_c20930 at 2171.453 kb on + strand, within PGA1_c20930 at 2171.467 kb on - strand at 2171.497 kb on - strand at 2171.506 kb on - strand at 2171.617 kb on + strand at 2171.625 kb on - strand at 2171.625 kb on - strand at 2171.630 kb on - strand at 2171.634 kb on + strand at 2171.637 kb on - strand at 2171.642 kb on - strand at 2171.661 kb on + strand at 2171.671 kb on + strand at 2171.673 kb on + strand at 2171.673 kb on + strand at 2171.673 kb on + strand at 2171.679 kb on - strand at 2171.679 kb on - strand at 2171.681 kb on - strand at 2171.681 kb on - strand at 2171.681 kb on - strand at 2171.696 kb on - strand at 2171.697 kb on + strand at 2171.705 kb on - strand at 2171.729 kb on + strand at 2171.769 kb on + strand at 2171.769 kb on + strand at 2171.789 kb on + strand at 2171.790 kb on + strand at 2171.821 kb on - strand at 2171.821 kb on - strand at 2171.828 kb on - strand at 2171.856 kb on + strand at 2171.859 kb on - strand at 2171.902 kb on - strand at 2171.954 kb on + strand, within PGA1_c20940 at 2171.993 kb on + strand, within PGA1_c20940 at 2171.996 kb on - strand, within PGA1_c20940 at 2172.001 kb on - strand, within PGA1_c20940 at 2172.090 kb on + strand, within PGA1_c20940 at 2172.093 kb on - strand, within PGA1_c20940 at 2172.141 kb on + strand, within PGA1_c20940 at 2172.144 kb on - strand, within PGA1_c20940 at 2172.144 kb on - strand, within PGA1_c20940 at 2172.144 kb on - strand, within PGA1_c20940 at 2172.149 kb on - strand, within PGA1_c20940 at 2172.190 kb on + strand, within PGA1_c20940 at 2172.202 kb on + strand, within PGA1_c20940 at 2172.202 kb on + strand, within PGA1_c20940 at 2172.202 kb on + strand, within PGA1_c20940 at 2172.202 kb on + strand, within PGA1_c20940 at 2172.391 kb on + strand, within PGA1_c20940 at 2172.433 kb on + strand, within PGA1_c20940 at 2172.527 kb on - strand, within PGA1_c20940 at 2172.533 kb on + strand, within PGA1_c20940 at 2172.541 kb on - strand, within PGA1_c20940 at 2172.589 kb on + strand, within PGA1_c20940 at 2172.629 kb on + strand, within PGA1_c20940 at 2172.633 kb on - strand, within PGA1_c20940
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 2,168,910 - +3.6 2,168,910 - -1.4 2,168,910 - +0.4 2,168,944 + +0.5 2,168,952 - -0.0 2,168,952 - +0.4 2,168,973 + +0.7 2,168,978 + -1.1 2,168,986 - +1.4 2,168,999 + +0.4 2,168,999 + +1.0 2,169,007 - +0.2 2,169,019 + -0.4 2,169,027 + -1.8 2,169,027 + -1.0 2,169,027 + +2.0 2,169,027 + -1.2 2,169,035 - +0.7 2,169,035 - +0.0 2,169,035 - -1.2 2,169,081 - +0.0 2,169,102 - +1.4 2,169,114 + +1.8 2,169,122 - -0.6 2,169,122 - -1.5 2,169,156 + -0.9 2,169,156 + +0.6 2,169,164 + -0.6 2,169,167 - -1.2 2,169,167 - +0.1 2,169,167 - -0.5 2,169,212 - -0.5 2,169,325 - PGA1_c20920 0.20 -0.8 2,169,335 - PGA1_c20920 0.22 -0.8 2,169,457 - PGA1_c20920 0.50 +0.0 2,169,541 - PGA1_c20920 0.69 -2.4 2,169,541 - PGA1_c20920 0.69 -2.4 2,169,545 + PGA1_c20920 0.70 -0.6 2,169,553 - PGA1_c20920 0.71 -0.2 2,169,698 - -0.7 2,169,962 - -4.8 2,169,982 + -3.6 2,170,010 - PGA1_c20930 0.11 -0.9 2,170,017 + PGA1_c20930 0.11 -3.3 2,170,022 + PGA1_c20930 0.11 -1.6 2,170,025 - PGA1_c20930 0.12 -0.6 2,170,090 + PGA1_c20930 0.15 -3.7 2,170,098 + PGA1_c20930 0.16 -3.7 2,170,172 + PGA1_c20930 0.20 -3.5 2,170,192 + PGA1_c20930 0.21 -3.6 2,170,207 + PGA1_c20930 0.22 -3.0 2,170,215 - PGA1_c20930 0.22 -1.7 2,170,261 + PGA1_c20930 0.25 -2.9 2,170,261 + PGA1_c20930 0.25 -2.6 2,170,261 + PGA1_c20930 0.25 -4.2 2,170,269 - PGA1_c20930 0.25 -3.4 2,170,269 - PGA1_c20930 0.25 -1.9 2,170,303 + PGA1_c20930 0.27 -1.9 2,170,333 + PGA1_c20930 0.28 -1.6 2,170,333 + PGA1_c20930 0.28 -2.6 2,170,333 + PGA1_c20930 0.28 -3.7 2,170,341 - PGA1_c20930 0.29 -1.9 2,170,342 - PGA1_c20930 0.29 -1.2 2,170,348 - PGA1_c20930 0.29 -0.7 2,170,360 + PGA1_c20930 0.30 -3.7 2,170,368 - PGA1_c20930 0.30 -1.4 2,170,375 + PGA1_c20930 0.31 -4.4 2,170,383 + PGA1_c20930 0.31 -2.7 2,170,391 - PGA1_c20930 0.32 -3.2 2,170,413 + PGA1_c20930 0.33 -3.9 2,170,437 + PGA1_c20930 0.34 -4.8 2,170,603 + PGA1_c20930 0.43 -1.2 2,170,611 - PGA1_c20930 0.44 -3.0 2,170,638 + PGA1_c20930 0.45 -1.6 2,170,638 + PGA1_c20930 0.45 -2.9 2,170,638 + PGA1_c20930 0.45 -2.0 2,170,638 + PGA1_c20930 0.45 -3.5 2,170,646 - PGA1_c20930 0.46 -1.7 2,170,646 - PGA1_c20930 0.46 -3.5 2,170,646 - PGA1_c20930 0.46 -2.7 2,170,646 - PGA1_c20930 0.46 -1.6 2,170,646 - PGA1_c20930 0.46 -2.7 2,170,707 - PGA1_c20930 0.49 -2.9 2,170,712 - PGA1_c20930 0.49 -2.2 2,170,726 - PGA1_c20930 0.50 -3.2 2,170,740 - PGA1_c20930 0.51 -1.6 2,170,743 + PGA1_c20930 0.51 -3.1 2,170,752 - PGA1_c20930 0.51 -2.1 2,170,759 + PGA1_c20930 0.52 -3.2 2,170,768 + PGA1_c20930 0.52 -3.6 2,170,768 + PGA1_c20930 0.52 -1.9 2,170,770 + PGA1_c20930 0.52 -3.9 2,170,770 + PGA1_c20930 0.52 -4.2 2,170,770 + PGA1_c20930 0.52 -2.7 2,170,776 - PGA1_c20930 0.53 -0.3 2,170,776 - PGA1_c20930 0.53 -3.4 2,170,776 - PGA1_c20930 0.53 -2.6 2,170,777 - PGA1_c20930 0.53 -3.8 2,170,778 - PGA1_c20930 0.53 -2.9 2,170,783 - PGA1_c20930 0.53 -2.9 2,170,805 + PGA1_c20930 0.54 -2.7 2,170,805 + PGA1_c20930 0.54 -2.7 2,170,805 + PGA1_c20930 0.54 -1.6 2,170,813 - PGA1_c20930 0.55 -2.6 2,170,813 - PGA1_c20930 0.55 -2.7 2,170,813 - PGA1_c20930 0.55 -3.3 2,170,820 - PGA1_c20930 0.55 -3.2 2,170,881 - PGA1_c20930 0.58 -1.2 2,170,898 + PGA1_c20930 0.59 -1.9 2,170,912 - PGA1_c20930 0.60 -3.6 2,170,939 + PGA1_c20930 0.62 -3.0 2,170,939 + PGA1_c20930 0.62 -3.8 2,171,049 + PGA1_c20930 0.68 -3.3 2,171,049 + PGA1_c20930 0.68 -2.4 2,171,049 + PGA1_c20930 0.68 -2.4 2,171,049 + PGA1_c20930 0.68 -4.0 2,171,049 + PGA1_c20930 0.68 -3.5 2,171,049 + PGA1_c20930 0.68 -2.5 2,171,049 + PGA1_c20930 0.68 -2.9 2,171,057 - PGA1_c20930 0.68 -4.0 2,171,057 - PGA1_c20930 0.68 -4.0 2,171,057 - PGA1_c20930 0.68 -1.2 2,171,057 - PGA1_c20930 0.68 -3.2 2,171,057 - PGA1_c20930 0.68 -1.7 2,171,067 - PGA1_c20930 0.69 -3.2 2,171,082 + PGA1_c20930 0.69 -4.7 2,171,220 + PGA1_c20930 0.77 -3.4 2,171,226 + PGA1_c20930 0.77 -3.2 2,171,226 + PGA1_c20930 0.77 -3.2 2,171,234 - PGA1_c20930 0.78 -3.6 2,171,240 - PGA1_c20930 0.78 -2.5 2,171,243 - PGA1_c20930 0.78 -3.4 2,171,302 + PGA1_c20930 0.81 -1.9 2,171,310 - PGA1_c20930 0.82 -2.4 2,171,324 + PGA1_c20930 0.83 -3.2 2,171,324 + PGA1_c20930 0.83 -1.9 2,171,326 + PGA1_c20930 0.83 -3.7 2,171,326 + PGA1_c20930 0.83 -4.2 2,171,326 + PGA1_c20930 0.83 -3.8 2,171,326 + PGA1_c20930 0.83 -3.3 2,171,326 + PGA1_c20930 0.83 -3.0 2,171,332 - PGA1_c20930 0.83 -4.0 2,171,332 - PGA1_c20930 0.83 -2.7 2,171,332 - PGA1_c20930 0.83 -4.2 2,171,334 - PGA1_c20930 0.83 -2.4 2,171,334 - PGA1_c20930 0.83 -2.6 2,171,334 - PGA1_c20930 0.83 -2.3 2,171,334 - PGA1_c20930 0.83 -2.5 2,171,334 - PGA1_c20930 0.83 -1.6 2,171,334 - PGA1_c20930 0.83 -2.4 2,171,334 - PGA1_c20930 0.83 -1.6 2,171,334 - PGA1_c20930 0.83 -3.0 2,171,334 - PGA1_c20930 0.83 -3.2 2,171,334 - PGA1_c20930 0.83 -3.3 2,171,334 - PGA1_c20930 0.83 -3.6 2,171,334 - PGA1_c20930 0.83 -2.4 2,171,334 - PGA1_c20930 0.83 -2.9 2,171,334 - PGA1_c20930 0.83 -2.9 2,171,334 - PGA1_c20930 0.83 -3.4 2,171,334 - PGA1_c20930 0.83 -4.0 2,171,334 - PGA1_c20930 0.83 -3.2 2,171,334 - PGA1_c20930 0.83 -0.9 2,171,334 - PGA1_c20930 0.83 -2.4 2,171,334 - PGA1_c20930 0.83 -2.2 2,171,390 + PGA1_c20930 0.86 -2.2 2,171,392 + PGA1_c20930 0.86 -3.4 2,171,453 + PGA1_c20930 0.90 -1.2 2,171,467 - -2.3 2,171,497 - -4.0 2,171,506 - -2.4 2,171,617 + -2.9 2,171,625 - -4.0 2,171,625 - +0.7 2,171,630 - -0.2 2,171,634 + -1.1 2,171,637 - -1.6 2,171,642 - +0.7 2,171,661 + -0.7 2,171,671 + -0.7 2,171,673 + +0.4 2,171,673 + +2.0 2,171,673 + -0.2 2,171,679 - +0.2 2,171,679 - -0.0 2,171,681 - +1.0 2,171,681 - +0.1 2,171,681 - -1.0 2,171,696 - -0.2 2,171,697 + +0.6 2,171,705 - +0.3 2,171,729 + -0.9 2,171,769 + +0.4 2,171,769 + +0.2 2,171,789 + -0.7 2,171,790 + +0.0 2,171,821 - -0.4 2,171,821 - -0.5 2,171,828 - +0.8 2,171,856 + -1.0 2,171,859 - -0.2 2,171,902 - -1.9 2,171,954 + PGA1_c20940 0.13 +0.9 2,171,993 + PGA1_c20940 0.16 +0.1 2,171,996 - PGA1_c20940 0.16 +1.0 2,172,001 - PGA1_c20940 0.17 +0.1 2,172,090 + PGA1_c20940 0.24 +0.4 2,172,093 - PGA1_c20940 0.24 +0.3 2,172,141 + PGA1_c20940 0.28 +0.4 2,172,144 - PGA1_c20940 0.28 -0.6 2,172,144 - PGA1_c20940 0.28 +0.3 2,172,144 - PGA1_c20940 0.28 -0.2 2,172,149 - PGA1_c20940 0.28 +0.6 2,172,190 + PGA1_c20940 0.32 +0.1 2,172,202 + PGA1_c20940 0.33 -0.4 2,172,202 + PGA1_c20940 0.33 +0.9 2,172,202 + PGA1_c20940 0.33 -0.8 2,172,202 + PGA1_c20940 0.33 -1.7 2,172,391 + PGA1_c20940 0.47 +0.6 2,172,433 + PGA1_c20940 0.51 -1.0 2,172,527 - PGA1_c20940 0.58 -0.7 2,172,533 + PGA1_c20940 0.59 -1.1 2,172,541 - PGA1_c20940 0.59 +0.2 2,172,589 + PGA1_c20940 0.63 +0.9 2,172,629 + PGA1_c20940 0.66 -1.6 2,172,633 - PGA1_c20940 0.66 -0.5
Or see this region's nucleotide sequence