Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17240 and PGA1_c17250 are separated by 280 nucleotides PGA1_c17250 and PGA1_c17260 are separated by 101 nucleotides PGA1_c17260 and PGA1_c17270 are separated by 71 nucleotides PGA1_c17270 and PGA1_c17280 are separated by 142 nucleotides
PGA1_c17240: PGA1_c17240 - Uncharacterized protein conserved in bacteria, at 1,800,236 to 1,801,186
_c17240
PGA1_c17250: PGA1_c17250 - glyceraldehyde-3-phosphate dehydrogenase 2, at 1,801,467 to 1,802,468
_c17250
PGA1_c17260: PGA1_c17260 - hypothetical protein, at 1,802,570 to 1,802,713
_c17260
PGA1_c17270: PGA1_c17270 - phosphopantetheine adenylyltransferase CoaA, at 1,802,785 to 1,803,279
_c17270
PGA1_c17280: PGA1_c17280 - cystathionine beta-synthase-like protein, at 1,803,422 to 1,803,856
_c17280
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1800.475 kb on + strand, within PGA1_c17240 at 1800.493 kb on - strand, within PGA1_c17240 at 1800.514 kb on - strand, within PGA1_c17240 at 1800.539 kb on + strand, within PGA1_c17240 at 1800.547 kb on - strand, within PGA1_c17240 at 1800.594 kb on - strand, within PGA1_c17240 at 1800.717 kb on + strand, within PGA1_c17240 at 1800.746 kb on + strand, within PGA1_c17240 at 1800.767 kb on - strand, within PGA1_c17240 at 1800.776 kb on + strand, within PGA1_c17240 at 1800.885 kb on - strand, within PGA1_c17240 at 1801.088 kb on - strand, within PGA1_c17240 at 1801.219 kb on + strand at 1801.221 kb on - strand at 1801.227 kb on - strand at 1801.232 kb on - strand at 1801.232 kb on - strand at 1801.245 kb on + strand at 1801.253 kb on - strand at 1801.292 kb on + strand at 1801.292 kb on + strand at 1801.318 kb on + strand at 1801.361 kb on + strand at 1801.369 kb on - strand at 1801.458 kb on - strand at 1801.516 kb on + strand at 1801.542 kb on - strand at 1801.559 kb on + strand at 1801.566 kb on + strand at 1801.670 kb on + strand, within PGA1_c17250 at 1801.716 kb on + strand, within PGA1_c17250 at 1801.724 kb on - strand, within PGA1_c17250 at 1801.751 kb on - strand, within PGA1_c17250 at 1801.790 kb on - strand, within PGA1_c17250 at 1801.795 kb on - strand, within PGA1_c17250 at 1801.806 kb on + strand, within PGA1_c17250 at 1801.823 kb on - strand, within PGA1_c17250 at 1801.823 kb on - strand, within PGA1_c17250 at 1801.850 kb on + strand, within PGA1_c17250 at 1801.925 kb on + strand, within PGA1_c17250 at 1801.943 kb on + strand, within PGA1_c17250 at 1802.064 kb on + strand, within PGA1_c17250 at 1802.111 kb on + strand, within PGA1_c17250 at 1802.335 kb on + strand, within PGA1_c17250 at 1802.352 kb on + strand, within PGA1_c17250 at 1802.360 kb on - strand, within PGA1_c17250 at 1802.360 kb on - strand, within PGA1_c17250 at 1802.360 kb on - strand, within PGA1_c17250 at 1802.414 kb on + strand at 1802.470 kb on - strand at 1802.471 kb on - strand at 1802.493 kb on + strand at 1802.519 kb on - strand at 1802.530 kb on + strand at 1802.588 kb on - strand, within PGA1_c17260 at 1802.608 kb on - strand, within PGA1_c17260 at 1802.680 kb on - strand, within PGA1_c17260 at 1803.344 kb on - strand at 1803.363 kb on + strand at 1803.391 kb on - strand at 1803.396 kb on + strand at 1803.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 1,800,475 + PGA1_c17240 0.25 -1.9 1,800,493 - PGA1_c17240 0.27 -1.6 1,800,514 - PGA1_c17240 0.29 +0.8 1,800,539 + PGA1_c17240 0.32 +0.8 1,800,547 - PGA1_c17240 0.33 -0.5 1,800,594 - PGA1_c17240 0.38 +0.3 1,800,717 + PGA1_c17240 0.51 +0.1 1,800,746 + PGA1_c17240 0.54 +0.1 1,800,767 - PGA1_c17240 0.56 +0.4 1,800,776 + PGA1_c17240 0.57 -2.2 1,800,885 - PGA1_c17240 0.68 -0.3 1,801,088 - PGA1_c17240 0.90 -0.3 1,801,219 + +0.3 1,801,221 - +1.6 1,801,227 - +0.2 1,801,232 - -0.2 1,801,232 - -0.7 1,801,245 + -0.6 1,801,253 - +1.2 1,801,292 + -0.5 1,801,292 + -1.0 1,801,318 + -0.6 1,801,361 + -0.4 1,801,369 - -0.6 1,801,458 - -1.8 1,801,516 + -1.9 1,801,542 - -1.2 1,801,559 + -3.6 1,801,566 + -1.8 1,801,670 + PGA1_c17250 0.20 -1.2 1,801,716 + PGA1_c17250 0.25 -2.8 1,801,724 - PGA1_c17250 0.26 -1.6 1,801,751 - PGA1_c17250 0.28 -2.7 1,801,790 - PGA1_c17250 0.32 -3.2 1,801,795 - PGA1_c17250 0.33 -3.9 1,801,806 + PGA1_c17250 0.34 -1.6 1,801,823 - PGA1_c17250 0.36 -1.9 1,801,823 - PGA1_c17250 0.36 -3.5 1,801,850 + PGA1_c17250 0.38 -1.8 1,801,925 + PGA1_c17250 0.46 -3.1 1,801,943 + PGA1_c17250 0.48 -2.4 1,802,064 + PGA1_c17250 0.60 -3.6 1,802,111 + PGA1_c17250 0.64 -2.8 1,802,335 + PGA1_c17250 0.87 -2.9 1,802,352 + PGA1_c17250 0.88 -2.4 1,802,360 - PGA1_c17250 0.89 -3.0 1,802,360 - PGA1_c17250 0.89 -1.9 1,802,360 - PGA1_c17250 0.89 -2.2 1,802,414 + -1.9 1,802,470 - -1.3 1,802,471 - -0.3 1,802,493 + +0.6 1,802,519 - +2.0 1,802,530 + -1.0 1,802,588 - PGA1_c17260 0.12 +1.2 1,802,608 - PGA1_c17260 0.26 -0.9 1,802,680 - PGA1_c17260 0.76 -0.4 1,803,344 - -0.7 1,803,363 + +0.4 1,803,391 - +0.3 1,803,396 + -0.4 1,803,437 - -3.1
Or see this region's nucleotide sequence