Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13270 and PGA1_c13280 are separated by 67 nucleotides PGA1_c13280 and PGA1_c13290 are separated by 229 nucleotides PGA1_c13290 and PGA1_c13300 are separated by 132 nucleotides PGA1_c13300 and PGA1_c13310 are separated by 136 nucleotides
PGA1_c13270: PGA1_c13270 - phosphoribosylglycinamide formyltransferase PurN, at 1,372,607 to 1,373,203
_c13270
PGA1_c13280: PGA1_c13280 - hypothetical protein, at 1,373,271 to 1,373,456
_c13280
PGA1_c13290: PGA1_c13290 - putative ribonuclease D, at 1,373,686 to 1,374,843
_c13290
PGA1_c13300: PGA1_c13300 - hypothetical protein, at 1,374,976 to 1,375,500
_c13300
PGA1_c13310: PGA1_c13310 - cysteine desulfuration protein sufE-like protein, at 1,375,637 to 1,376,050
_c13310
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1372.731 kb on + strand, within PGA1_c13270 at 1372.811 kb on + strand, within PGA1_c13270 at 1372.885 kb on + strand, within PGA1_c13270 at 1372.893 kb on - strand, within PGA1_c13270 at 1372.893 kb on - strand, within PGA1_c13270 at 1372.941 kb on + strand, within PGA1_c13270 at 1373.065 kb on - strand, within PGA1_c13270 at 1373.068 kb on + strand, within PGA1_c13270 at 1373.197 kb on + strand at 1373.197 kb on + strand at 1373.200 kb on - strand at 1373.269 kb on + strand at 1373.339 kb on + strand, within PGA1_c13280 at 1373.347 kb on - strand, within PGA1_c13280 at 1373.347 kb on - strand, within PGA1_c13280 at 1373.380 kb on - strand, within PGA1_c13280 at 1373.395 kb on - strand, within PGA1_c13280 at 1373.429 kb on + strand, within PGA1_c13280 at 1373.429 kb on + strand, within PGA1_c13280 at 1373.429 kb on + strand, within PGA1_c13280 at 1373.429 kb on + strand, within PGA1_c13280 at 1373.449 kb on - strand at 1373.449 kb on - strand at 1373.449 kb on - strand at 1373.449 kb on - strand at 1373.449 kb on - strand at 1373.487 kb on + strand at 1373.487 kb on + strand at 1373.487 kb on + strand at 1373.487 kb on + strand at 1373.487 kb on + strand at 1373.487 kb on + strand at 1373.495 kb on - strand at 1373.495 kb on - strand at 1373.495 kb on - strand at 1373.495 kb on - strand at 1373.563 kb on + strand at 1373.577 kb on + strand at 1373.617 kb on - strand at 1373.626 kb on - strand at 1373.633 kb on + strand at 1373.633 kb on + strand at 1373.633 kb on + strand at 1373.633 kb on + strand at 1373.639 kb on + strand at 1373.641 kb on - strand at 1374.822 kb on + strand at 1374.850 kb on - strand at 1374.958 kb on - strand at 1374.968 kb on - strand at 1375.079 kb on - strand, within PGA1_c13300 at 1375.081 kb on - strand, within PGA1_c13300 at 1375.090 kb on + strand, within PGA1_c13300 at 1375.090 kb on + strand, within PGA1_c13300 at 1375.090 kb on + strand, within PGA1_c13300 at 1375.098 kb on - strand, within PGA1_c13300 at 1375.154 kb on - strand, within PGA1_c13300 at 1375.280 kb on + strand, within PGA1_c13300 at 1375.287 kb on - strand, within PGA1_c13300 at 1375.315 kb on - strand, within PGA1_c13300 at 1375.336 kb on + strand, within PGA1_c13300 at 1375.378 kb on - strand, within PGA1_c13300 at 1375.383 kb on - strand, within PGA1_c13300 at 1375.392 kb on + strand, within PGA1_c13300 at 1375.403 kb on - strand, within PGA1_c13300 at 1375.404 kb on + strand, within PGA1_c13300 at 1375.404 kb on + strand, within PGA1_c13300 at 1375.482 kb on + strand at 1375.530 kb on - strand at 1375.554 kb on + strand at 1375.586 kb on + strand at 1375.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 1,372,731 + PGA1_c13270 0.21 -2.3 1,372,811 + PGA1_c13270 0.34 -1.9 1,372,885 + PGA1_c13270 0.47 -1.6 1,372,893 - PGA1_c13270 0.48 -2.6 1,372,893 - PGA1_c13270 0.48 -3.7 1,372,941 + PGA1_c13270 0.56 -2.4 1,373,065 - PGA1_c13270 0.77 -3.2 1,373,068 + PGA1_c13270 0.77 -0.6 1,373,197 + +0.4 1,373,197 + -1.8 1,373,200 - -2.4 1,373,269 + -1.2 1,373,339 + PGA1_c13280 0.37 -0.9 1,373,347 - PGA1_c13280 0.41 -1.8 1,373,347 - PGA1_c13280 0.41 -2.7 1,373,380 - PGA1_c13280 0.59 -0.7 1,373,395 - PGA1_c13280 0.67 -1.2 1,373,429 + PGA1_c13280 0.85 -1.2 1,373,429 + PGA1_c13280 0.85 -2.0 1,373,429 + PGA1_c13280 0.85 -2.1 1,373,429 + PGA1_c13280 0.85 -1.2 1,373,449 - -0.3 1,373,449 - -1.5 1,373,449 - -0.0 1,373,449 - -1.2 1,373,449 - -0.2 1,373,487 + -1.6 1,373,487 + -1.4 1,373,487 + -2.2 1,373,487 + -1.2 1,373,487 + -2.2 1,373,487 + -0.4 1,373,495 - -2.2 1,373,495 - -1.8 1,373,495 - -1.9 1,373,495 - -0.2 1,373,563 + +0.2 1,373,577 + -1.3 1,373,617 - +0.2 1,373,626 - -0.7 1,373,633 + -0.2 1,373,633 + +0.8 1,373,633 + -0.9 1,373,633 + -0.1 1,373,639 + +0.5 1,373,641 - +0.2 1,374,822 + -1.2 1,374,850 - -0.4 1,374,958 - -0.6 1,374,968 - -1.3 1,375,079 - PGA1_c13300 0.20 -0.7 1,375,081 - PGA1_c13300 0.20 +0.2 1,375,090 + PGA1_c13300 0.22 +0.8 1,375,090 + PGA1_c13300 0.22 +0.1 1,375,090 + PGA1_c13300 0.22 -1.2 1,375,098 - PGA1_c13300 0.23 +0.5 1,375,154 - PGA1_c13300 0.34 -0.6 1,375,280 + PGA1_c13300 0.58 +0.7 1,375,287 - PGA1_c13300 0.59 -0.2 1,375,315 - PGA1_c13300 0.65 +1.5 1,375,336 + PGA1_c13300 0.69 -0.8 1,375,378 - PGA1_c13300 0.77 -0.8 1,375,383 - PGA1_c13300 0.78 +0.1 1,375,392 + PGA1_c13300 0.79 +1.7 1,375,403 - PGA1_c13300 0.81 -1.1 1,375,404 + PGA1_c13300 0.82 +0.6 1,375,404 + PGA1_c13300 0.82 +1.0 1,375,482 + +0.6 1,375,530 - +0.0 1,375,554 + -0.3 1,375,586 + +0.3 1,375,600 - -0.3
Or see this region's nucleotide sequence