Experiment: PYE 0.5x 25C survival rep1 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03893 and CCNA_01721 are separated by 22 nucleotides CCNA_01721 and CCNA_01722 overlap by 32 nucleotides CCNA_01722 and CCNA_01723 are separated by 92 nucleotides
CCNA_03893: CCNA_03893 - hypothetical protein, at 1,848,134 to 1,848,526
_03893
CCNA_01721: CCNA_01721 - RNA polymerase ECF sigma factor, at 1,848,549 to 1,849,133
_01721
CCNA_01722: CCNA_01722 - hypothetical protein, at 1,849,102 to 1,849,506
_01722
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
Position (kb)
1849
1850 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1848.189 kb on - strand, within CCNA_03893 at 1848.268 kb on + strand, within CCNA_03893 at 1848.292 kb on + strand, within CCNA_03893 at 1848.323 kb on + strand, within CCNA_03893 at 1848.331 kb on - strand, within CCNA_03893 at 1848.386 kb on + strand, within CCNA_03893 at 1848.461 kb on + strand, within CCNA_03893 at 1848.495 kb on + strand at 1848.495 kb on + strand at 1848.520 kb on + strand at 1848.520 kb on + strand at 1848.536 kb on + strand at 1848.537 kb on + strand at 1848.589 kb on + strand at 1848.597 kb on - strand at 1848.614 kb on - strand, within CCNA_01721 at 1848.629 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.674 kb on - strand, within CCNA_01721 at 1848.675 kb on - strand, within CCNA_01721 at 1848.731 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.827 kb on + strand, within CCNA_01721 at 1848.881 kb on - strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.988 kb on + strand, within CCNA_01721 at 1849.044 kb on + strand, within CCNA_01721 at 1849.052 kb on - strand, within CCNA_01721 at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.130 kb on - strand at 1849.198 kb on + strand at 1849.200 kb on + strand, within CCNA_01722 at 1849.277 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.520 kb on + strand at 1850.421 kb on + strand at 1850.454 kb on + strand at 1850.454 kb on + strand at 1850.456 kb on + strand at 1850.462 kb on - strand at 1850.503 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 25C survival rep1 12 days remove 1,848,189 - CCNA_03893 0.14 +1.1 1,848,268 + CCNA_03893 0.34 +0.4 1,848,292 + CCNA_03893 0.40 +0.9 1,848,323 + CCNA_03893 0.48 +3.7 1,848,331 - CCNA_03893 0.50 +0.0 1,848,386 + CCNA_03893 0.64 -0.5 1,848,461 + CCNA_03893 0.83 -0.5 1,848,495 + -0.0 1,848,495 + -2.5 1,848,520 + -1.3 1,848,520 + -1.9 1,848,536 + -5.6 1,848,537 + -1.4 1,848,589 + +0.3 1,848,597 - +1.7 1,848,614 - CCNA_01721 0.11 +1.1 1,848,629 + CCNA_01721 0.14 +0.7 1,848,666 + CCNA_01721 0.20 -0.1 1,848,674 - CCNA_01721 0.21 +1.1 1,848,675 - CCNA_01721 0.22 +1.1 1,848,731 + CCNA_01721 0.31 +1.7 1,848,812 + CCNA_01721 0.45 +0.7 1,848,812 + CCNA_01721 0.45 +1.7 1,848,820 - CCNA_01721 0.46 +0.3 1,848,820 - CCNA_01721 0.46 +1.1 1,848,827 + CCNA_01721 0.48 -0.1 1,848,881 - CCNA_01721 0.57 +1.1 1,848,936 + CCNA_01721 0.66 +0.5 1,848,936 + CCNA_01721 0.66 +0.0 1,848,988 + CCNA_01721 0.75 +0.7 1,849,044 + CCNA_01721 0.85 +0.5 1,849,052 - CCNA_01721 0.86 +2.5 1,849,122 + +1.1 1,849,122 + +0.3 1,849,122 + +0.7 1,849,122 + +0.4 1,849,130 - +0.3 1,849,198 + -1.0 1,849,200 + CCNA_01722 0.24 -4.7 1,849,277 + CCNA_01722 0.43 -3.5 1,849,282 + CCNA_01722 0.44 -4.5 1,849,282 + CCNA_01722 0.44 -3.9 1,849,520 + -0.1 1,850,421 + -1.3 1,850,454 + -1.2 1,850,454 + +0.0 1,850,456 + +0.6 1,850,462 - +1.7 1,850,503 + -2.2
Or see this region's nucleotide sequence