Experiment: PYE 0.5x 25C survival rep1 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01220 and CCNA_01221 are separated by 3 nucleotides CCNA_01221 and CCNA_01222 are separated by 67 nucleotides
CCNA_01220: CCNA_01220 - 7-keto-8-aminopelargonate synthetase, at 1,346,517 to 1,347,731
_01220
CCNA_01221: CCNA_01221 - acyl carrier protein, at 1,347,735 to 1,347,995
_01221
CCNA_01222: CCNA_01222 - NADH-ubiquinone oxidoreductase, at 1,348,063 to 1,349,016
_01222
Position (kb)
1347
1348 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1346.759 kb on + strand, within CCNA_01220 at 1346.767 kb on - strand, within CCNA_01220 at 1346.803 kb on + strand, within CCNA_01220 at 1346.846 kb on - strand, within CCNA_01220 at 1346.998 kb on - strand, within CCNA_01220 at 1347.038 kb on - strand, within CCNA_01220 at 1347.078 kb on + strand, within CCNA_01220 at 1347.108 kb on - strand, within CCNA_01220 at 1347.158 kb on + strand, within CCNA_01220 at 1347.251 kb on - strand, within CCNA_01220 at 1347.291 kb on + strand, within CCNA_01220 at 1347.291 kb on + strand, within CCNA_01220 at 1347.299 kb on - strand, within CCNA_01220 at 1347.314 kb on - strand, within CCNA_01220 at 1347.325 kb on - strand, within CCNA_01220 at 1347.526 kb on + strand, within CCNA_01220 at 1347.554 kb on + strand, within CCNA_01220 at 1347.614 kb on - strand at 1347.627 kb on - strand at 1347.648 kb on - strand at 1347.661 kb on - strand at 1347.685 kb on + strand at 1347.685 kb on + strand at 1347.693 kb on - strand at 1347.693 kb on - strand at 1347.705 kb on + strand at 1347.747 kb on - strand at 1347.754 kb on + strand at 1347.762 kb on - strand, within CCNA_01221 at 1347.821 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.932 kb on - strand, within CCNA_01221 at 1347.941 kb on + strand, within CCNA_01221 at 1347.949 kb on - strand, within CCNA_01221 at 1347.955 kb on - strand, within CCNA_01221 at 1347.963 kb on + strand, within CCNA_01221 at 1347.970 kb on - strand at 1347.980 kb on + strand at 1347.998 kb on - strand at 1347.998 kb on - strand at 1348.172 kb on + strand, within CCNA_01222 at 1348.193 kb on + strand, within CCNA_01222 at 1348.205 kb on + strand, within CCNA_01222 at 1348.206 kb on + strand, within CCNA_01222 at 1348.206 kb on + strand, within CCNA_01222 at 1348.214 kb on - strand, within CCNA_01222 at 1348.222 kb on + strand, within CCNA_01222 at 1348.230 kb on - strand, within CCNA_01222 at 1348.323 kb on - strand, within CCNA_01222 at 1348.333 kb on - strand, within CCNA_01222 at 1348.333 kb on - strand, within CCNA_01222 at 1348.366 kb on - strand, within CCNA_01222 at 1348.401 kb on + strand, within CCNA_01222 at 1348.459 kb on + strand, within CCNA_01222 at 1348.467 kb on - strand, within CCNA_01222 at 1348.525 kb on - strand, within CCNA_01222 at 1348.525 kb on - strand, within CCNA_01222 at 1348.527 kb on + strand, within CCNA_01222 at 1348.553 kb on - strand, within CCNA_01222 at 1348.553 kb on - strand, within CCNA_01222 at 1348.616 kb on + strand, within CCNA_01222 at 1348.662 kb on + strand, within CCNA_01222 at 1348.677 kb on - strand, within CCNA_01222 at 1348.717 kb on - strand, within CCNA_01222 at 1348.746 kb on + strand, within CCNA_01222 at 1348.755 kb on + strand, within CCNA_01222 at 1348.763 kb on + strand, within CCNA_01222 at 1348.800 kb on - strand, within CCNA_01222 at 1348.820 kb on - strand, within CCNA_01222 at 1348.825 kb on - strand, within CCNA_01222 at 1348.837 kb on - strand, within CCNA_01222 at 1348.867 kb on - strand, within CCNA_01222 at 1348.880 kb on - strand, within CCNA_01222
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 25C survival rep1 12 days remove 1,346,759 + CCNA_01220 0.20 +2.4 1,346,767 - CCNA_01220 0.21 -1.8 1,346,803 + CCNA_01220 0.24 +2.4 1,346,846 - CCNA_01220 0.27 -4.4 1,346,998 - CCNA_01220 0.40 -3.1 1,347,038 - CCNA_01220 0.43 +0.8 1,347,078 + CCNA_01220 0.46 +2.4 1,347,108 - CCNA_01220 0.49 -3.4 1,347,158 + CCNA_01220 0.53 +2.4 1,347,251 - CCNA_01220 0.60 -2.6 1,347,291 + CCNA_01220 0.64 +2.4 1,347,291 + CCNA_01220 0.64 +0.8 1,347,299 - CCNA_01220 0.64 -1.8 1,347,314 - CCNA_01220 0.66 -0.8 1,347,325 - CCNA_01220 0.67 -2.8 1,347,526 + CCNA_01220 0.83 +0.4 1,347,554 + CCNA_01220 0.85 +2.4 1,347,614 - -0.2 1,347,627 - -1.3 1,347,648 - -2.9 1,347,661 - -1.3 1,347,685 + +2.4 1,347,685 + +2.4 1,347,693 - -2.8 1,347,693 - -1.4 1,347,705 + +1.4 1,347,747 - -2.4 1,347,754 + +1.4 1,347,762 - CCNA_01221 0.10 -0.3 1,347,821 + CCNA_01221 0.33 +2.4 1,347,924 + CCNA_01221 0.72 +0.4 1,347,924 + CCNA_01221 0.72 -0.2 1,347,924 + CCNA_01221 0.72 +1.4 1,347,932 - CCNA_01221 0.75 -1.1 1,347,941 + CCNA_01221 0.79 +0.4 1,347,949 - CCNA_01221 0.82 -2.4 1,347,955 - CCNA_01221 0.84 -2.5 1,347,963 + CCNA_01221 0.87 +2.4 1,347,970 - -2.3 1,347,980 + -0.6 1,347,998 - +0.8 1,347,998 - +0.7 1,348,172 + CCNA_01222 0.11 -0.8 1,348,193 + CCNA_01222 0.14 +0.1 1,348,205 + CCNA_01222 0.15 +0.4 1,348,206 + CCNA_01222 0.15 +2.4 1,348,206 + CCNA_01222 0.15 +2.4 1,348,214 - CCNA_01222 0.16 -2.3 1,348,222 + CCNA_01222 0.17 +2.4 1,348,230 - CCNA_01222 0.18 -0.2 1,348,323 - CCNA_01222 0.27 -3.4 1,348,333 - CCNA_01222 0.28 +0.1 1,348,333 - CCNA_01222 0.28 -3.4 1,348,366 - CCNA_01222 0.32 -1.1 1,348,401 + CCNA_01222 0.35 -0.6 1,348,459 + CCNA_01222 0.42 +1.4 1,348,467 - CCNA_01222 0.42 +0.8 1,348,525 - CCNA_01222 0.48 -1.5 1,348,525 - CCNA_01222 0.48 -1.9 1,348,527 + CCNA_01222 0.49 +2.4 1,348,553 - CCNA_01222 0.51 +0.4 1,348,553 - CCNA_01222 0.51 -1.3 1,348,616 + CCNA_01222 0.58 +0.4 1,348,662 + CCNA_01222 0.63 -1.1 1,348,677 - CCNA_01222 0.64 +0.8 1,348,717 - CCNA_01222 0.69 -3.2 1,348,746 + CCNA_01222 0.72 -3.6 1,348,755 + CCNA_01222 0.73 -1.1 1,348,763 + CCNA_01222 0.73 +0.8 1,348,800 - CCNA_01222 0.77 +0.4 1,348,820 - CCNA_01222 0.79 -2.8 1,348,825 - CCNA_01222 0.80 -2.3 1,348,837 - CCNA_01222 0.81 +0.8 1,348,867 - CCNA_01222 0.84 +0.4 1,348,880 - CCNA_01222 0.86 +2.4
Or see this region's nucleotide sequence