Experiment: PYE 0.5x 25C survival rep1 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00151 and CCNA_00152 are separated by 139 nucleotides CCNA_00152 and CCNA_00153 are separated by 12 nucleotides CCNA_00153 and CCNA_00154 overlap by 61 nucleotides
CCNA_00151: CCNA_00151 - ribonuclease PH, at 160,464 to 161,180
_00151
CCNA_00152: CCNA_00152 - winged helix-turn-helix transcription repressor HrcA, at 161,320 to 162,396
_00152
CCNA_00153: CCNA_00153 - adenine nucleotide exchange factor GrpE, at 162,409 to 163,035
_00153
CCNA_00154: CCNA_00154 - hypothetical protein, at 162,975 to 163,235
_00154
Position (kb) 161
162
163 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 160.329 kb on + strand at 160.337 kb on - strand at 160.396 kb on - strand at 160.405 kb on + strand at 160.406 kb on - strand at 160.406 kb on - strand at 160.413 kb on + strand at 160.426 kb on + strand at 160.455 kb on + strand at 160.455 kb on + strand at 160.455 kb on + strand at 160.456 kb on + strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.463 kb on - strand at 160.480 kb on + strand at 160.511 kb on - strand at 160.520 kb on - strand at 160.571 kb on - strand, within CCNA_00151 at 160.571 kb on - strand, within CCNA_00151 at 160.605 kb on + strand, within CCNA_00151 at 160.641 kb on - strand, within CCNA_00151 at 160.646 kb on - strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.675 kb on + strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.683 kb on - strand, within CCNA_00151 at 160.699 kb on + strand, within CCNA_00151 at 160.726 kb on + strand, within CCNA_00151 at 160.734 kb on - strand, within CCNA_00151 at 160.755 kb on - strand, within CCNA_00151 at 160.761 kb on + strand at 160.774 kb on - strand, within CCNA_00151 at 160.780 kb on - strand, within CCNA_00151 at 160.796 kb on - strand, within CCNA_00151 at 160.848 kb on - strand, within CCNA_00151 at 160.864 kb on + strand, within CCNA_00151 at 160.873 kb on - strand, within CCNA_00151 at 160.940 kb on - strand, within CCNA_00151 at 160.989 kb on + strand, within CCNA_00151 at 160.999 kb on + strand, within CCNA_00151 at 160.999 kb on + strand, within CCNA_00151 at 161.002 kb on - strand, within CCNA_00151 at 161.007 kb on - strand, within CCNA_00151 at 161.025 kb on - strand, within CCNA_00151 at 161.046 kb on + strand, within CCNA_00151 at 161.054 kb on - strand, within CCNA_00151 at 161.054 kb on - strand, within CCNA_00151 at 161.067 kb on - strand, within CCNA_00151 at 161.072 kb on - strand, within CCNA_00151 at 161.079 kb on - strand, within CCNA_00151 at 161.089 kb on - strand, within CCNA_00151 at 161.104 kb on - strand, within CCNA_00151 at 161.106 kb on - strand, within CCNA_00151 at 161.135 kb on - strand at 161.135 kb on - strand at 161.143 kb on + strand at 161.143 kb on + strand at 161.151 kb on - strand at 161.151 kb on - strand at 161.161 kb on - strand at 161.187 kb on + strand at 161.187 kb on + strand at 161.195 kb on - strand at 161.205 kb on + strand at 161.221 kb on - strand at 161.221 kb on - strand at 161.221 kb on - strand at 161.244 kb on - strand at 161.277 kb on - strand at 161.323 kb on + strand at 161.328 kb on + strand at 161.328 kb on + strand at 161.331 kb on - strand at 161.333 kb on + strand at 161.336 kb on - strand at 161.336 kb on - strand at 161.336 kb on - strand at 161.341 kb on - strand at 161.348 kb on + strand at 161.354 kb on + strand at 161.354 kb on + strand at 161.362 kb on - strand at 161.362 kb on - strand at 161.384 kb on + strand at 161.400 kb on - strand at 161.412 kb on + strand at 161.412 kb on + strand at 161.426 kb on + strand at 161.450 kb on - strand, within CCNA_00152 at 161.452 kb on + strand, within CCNA_00152 at 161.513 kb on - strand, within CCNA_00152 at 161.515 kb on - strand, within CCNA_00152 at 161.516 kb on + strand, within CCNA_00152 at 161.552 kb on + strand, within CCNA_00152 at 161.685 kb on + strand, within CCNA_00152 at 161.685 kb on + strand, within CCNA_00152 at 161.704 kb on - strand, within CCNA_00152 at 161.705 kb on + strand, within CCNA_00152 at 161.710 kb on + strand, within CCNA_00152 at 161.713 kb on - strand, within CCNA_00152 at 161.760 kb on + strand, within CCNA_00152 at 161.782 kb on + strand, within CCNA_00152 at 161.817 kb on + strand, within CCNA_00152 at 161.841 kb on - strand, within CCNA_00152 at 161.841 kb on - strand, within CCNA_00152 at 161.866 kb on + strand, within CCNA_00152 at 161.929 kb on - strand, within CCNA_00152 at 161.959 kb on + strand, within CCNA_00152 at 161.967 kb on - strand, within CCNA_00152 at 162.025 kb on + strand, within CCNA_00152 at 162.064 kb on - strand, within CCNA_00152 at 162.075 kb on + strand, within CCNA_00152 at 162.122 kb on + strand, within CCNA_00152 at 162.140 kb on + strand, within CCNA_00152 at 162.140 kb on + strand, within CCNA_00152 at 162.147 kb on + strand, within CCNA_00152 at 162.171 kb on + strand, within CCNA_00152 at 162.171 kb on + strand, within CCNA_00152 at 162.171 kb on + strand, within CCNA_00152 at 162.171 kb on + strand, within CCNA_00152 at 162.179 kb on - strand, within CCNA_00152 at 162.239 kb on - strand, within CCNA_00152 at 162.264 kb on + strand, within CCNA_00152 at 162.267 kb on + strand, within CCNA_00152 at 162.269 kb on + strand, within CCNA_00152 at 162.340 kb on - strand at 162.347 kb on - strand at 162.354 kb on + strand at 162.392 kb on + strand at 162.392 kb on + strand at 162.392 kb on + strand at 162.405 kb on - strand at 162.937 kb on - strand, within CCNA_00153 at 162.952 kb on - strand, within CCNA_00153 at 162.972 kb on + strand, within CCNA_00153 at 162.973 kb on - strand at 162.980 kb on - strand at 162.981 kb on - strand at 163.031 kb on - strand at 163.104 kb on + strand, within CCNA_00154 at 163.112 kb on - strand, within CCNA_00154 at 163.113 kb on - strand, within CCNA_00154 at 163.117 kb on + strand, within CCNA_00154 at 163.117 kb on + strand at 163.117 kb on + strand, within CCNA_00154 at 163.158 kb on + strand, within CCNA_00154 at 163.165 kb on + strand, within CCNA_00154 at 163.171 kb on - strand, within CCNA_00154 at 163.171 kb on - strand, within CCNA_00154 at 163.178 kb on - strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.209 kb on + strand, within CCNA_00154 at 163.217 kb on - strand at 163.217 kb on - strand at 163.217 kb on - strand at 163.217 kb on - strand at 163.217 kb on - strand at 163.217 kb on - strand at 163.217 kb on - strand at 163.218 kb on + strand at 163.218 kb on + strand at 163.218 kb on + strand at 163.223 kb on + strand at 163.226 kb on - strand at 163.226 kb on - strand at 163.226 kb on - strand at 163.231 kb on - strand at 163.233 kb on + strand at 163.289 kb on - strand at 163.297 kb on - strand at 163.304 kb on - strand at 163.348 kb on + strand at 163.365 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 25C survival rep1 12 days remove 160,329 + -0.1 160,337 - +1.8 160,396 - +0.3 160,405 + +0.7 160,406 - +0.7 160,406 - +0.4 160,413 + +0.2 160,426 + -0.1 160,455 + +1.1 160,455 + +0.1 160,455 + +0.5 160,456 + -1.8 160,463 - +0.4 160,463 - +1.1 160,463 - +0.6 160,463 - +0.6 160,463 - +0.1 160,480 + -1.1 160,511 - -0.9 160,520 - -1.2 160,571 - CCNA_00151 0.15 -0.6 160,571 - CCNA_00151 0.15 +0.1 160,605 + CCNA_00151 0.20 -2.0 160,641 - CCNA_00151 0.25 -1.5 160,646 - CCNA_00151 0.25 -0.5 160,675 + CCNA_00151 0.29 -0.7 160,675 + CCNA_00151 0.29 -1.9 160,675 + CCNA_00151 0.29 -0.5 160,683 - CCNA_00151 0.31 -1.1 160,683 - CCNA_00151 0.31 -1.8 160,683 - CCNA_00151 0.31 -0.4 160,683 - CCNA_00151 0.31 -1.1 160,683 - CCNA_00151 0.31 -1.4 160,683 - CCNA_00151 0.31 -1.8 160,683 - CCNA_00151 0.31 -0.5 160,699 + CCNA_00151 0.33 -2.5 160,726 + CCNA_00151 0.37 -0.7 160,734 - CCNA_00151 0.38 -0.4 160,755 - CCNA_00151 0.41 -0.8 160,761 + -0.2 160,774 - CCNA_00151 0.43 -0.1 160,780 - CCNA_00151 0.44 +0.1 160,796 - CCNA_00151 0.46 -1.2 160,848 - CCNA_00151 0.54 +0.0 160,864 + CCNA_00151 0.56 -2.1 160,873 - CCNA_00151 0.57 -0.8 160,940 - CCNA_00151 0.66 -1.1 160,989 + CCNA_00151 0.73 -1.1 160,999 + CCNA_00151 0.75 +2.1 160,999 + CCNA_00151 0.75 -1.8 161,002 - CCNA_00151 0.75 -0.0 161,007 - CCNA_00151 0.76 -1.2 161,025 - CCNA_00151 0.78 -2.2 161,046 + CCNA_00151 0.81 -1.3 161,054 - CCNA_00151 0.82 -1.3 161,054 - CCNA_00151 0.82 -1.3 161,067 - CCNA_00151 0.84 -1.0 161,072 - CCNA_00151 0.85 -0.3 161,079 - CCNA_00151 0.86 +0.3 161,089 - CCNA_00151 0.87 -1.5 161,104 - CCNA_00151 0.89 -0.2 161,106 - CCNA_00151 0.90 -0.3 161,135 - -0.9 161,135 - -0.9 161,143 + -2.8 161,143 + -2.5 161,151 - -1.1 161,151 - -0.6 161,161 - -0.3 161,187 + +0.3 161,187 + -1.1 161,195 - +0.9 161,205 + -0.7 161,221 - -2.1 161,221 - -2.4 161,221 - -0.9 161,244 - +0.1 161,277 - -1.5 161,323 + -0.7 161,328 + -1.5 161,328 + -1.1 161,331 - -0.7 161,333 + -1.4 161,336 - -0.5 161,336 - -1.1 161,336 - -1.6 161,341 - -0.9 161,348 + -1.7 161,354 + +2.1 161,354 + -0.5 161,362 - +0.5 161,362 - -2.3 161,384 + -1.9 161,400 - -1.4 161,412 + -3.0 161,412 + -1.1 161,426 + +0.0 161,450 - CCNA_00152 0.12 -1.1 161,452 + CCNA_00152 0.12 -2.9 161,513 - CCNA_00152 0.18 +2.1 161,515 - CCNA_00152 0.18 +1.1 161,516 + CCNA_00152 0.18 -3.3 161,552 + CCNA_00152 0.22 -1.4 161,685 + CCNA_00152 0.34 -1.4 161,685 + CCNA_00152 0.34 -4.9 161,704 - CCNA_00152 0.36 -1.6 161,705 + CCNA_00152 0.36 -0.8 161,710 + CCNA_00152 0.36 -3.2 161,713 - CCNA_00152 0.36 -2.4 161,760 + CCNA_00152 0.41 -0.2 161,782 + CCNA_00152 0.43 -1.5 161,817 + CCNA_00152 0.46 -1.0 161,841 - CCNA_00152 0.48 +0.1 161,841 - CCNA_00152 0.48 +0.5 161,866 + CCNA_00152 0.51 -1.6 161,929 - CCNA_00152 0.57 +1.1 161,959 + CCNA_00152 0.59 -2.9 161,967 - CCNA_00152 0.60 -0.5 162,025 + CCNA_00152 0.65 -2.7 162,064 - CCNA_00152 0.69 -2.8 162,075 + CCNA_00152 0.70 -0.9 162,122 + CCNA_00152 0.74 -3.1 162,140 + CCNA_00152 0.76 -2.0 162,140 + CCNA_00152 0.76 +1.1 162,147 + CCNA_00152 0.77 -3.4 162,171 + CCNA_00152 0.79 -1.0 162,171 + CCNA_00152 0.79 +1.4 162,171 + CCNA_00152 0.79 -2.3 162,171 + CCNA_00152 0.79 -0.2 162,179 - CCNA_00152 0.80 -2.2 162,239 - CCNA_00152 0.85 -1.1 162,264 + CCNA_00152 0.88 -4.4 162,267 + CCNA_00152 0.88 -2.8 162,269 + CCNA_00152 0.88 -3.1 162,340 - -1.5 162,347 - -1.4 162,354 + -2.5 162,392 + -0.3 162,392 + +0.5 162,392 + -0.1 162,405 - +0.3 162,937 - CCNA_00153 0.84 -1.4 162,952 - CCNA_00153 0.87 -3.0 162,972 + CCNA_00153 0.90 -1.4 162,973 - -1.0 162,980 - -2.4 162,981 - -4.2 163,031 - -0.6 163,104 + CCNA_00154 0.49 +1.5 163,112 - CCNA_00154 0.52 -0.1 163,113 - CCNA_00154 0.53 -0.4 163,117 + CCNA_00154 0.54 +0.4 163,117 + +0.5 163,117 + CCNA_00154 0.54 +0.4 163,158 + CCNA_00154 0.70 -0.1 163,165 + CCNA_00154 0.73 +0.1 163,171 - CCNA_00154 0.75 -0.1 163,171 - CCNA_00154 0.75 +0.1 163,178 - CCNA_00154 0.78 -0.6 163,209 + CCNA_00154 0.90 +0.3 163,209 + CCNA_00154 0.90 +0.1 163,209 + CCNA_00154 0.90 -0.3 163,209 + CCNA_00154 0.90 -0.3 163,209 + CCNA_00154 0.90 -0.0 163,209 + CCNA_00154 0.90 -0.6 163,217 - +0.3 163,217 - -0.7 163,217 - -0.2 163,217 - -0.3 163,217 - -1.2 163,217 - -0.3 163,217 - -2.3 163,218 + -1.1 163,218 + -1.2 163,218 + +0.3 163,223 + -0.2 163,226 - -0.8 163,226 - -1.1 163,226 - -0.1 163,231 - +0.6 163,233 + -0.8 163,289 - +0.3 163,297 - -0.2 163,304 - -1.2 163,348 + -1.2 163,365 + -2.5
Or see this region's nucleotide sequence