Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3005 and Dsui_3006 are separated by 136 nucleotides Dsui_3006 and Dsui_3007 are separated by 4 nucleotides Dsui_3007 and Dsui_3008 overlap by 1 nucleotides Dsui_3008 and Dsui_3009 are separated by 128 nucleotides
Dsui_3005: Dsui_3005 - transcription elongation factor GreB, at 3,219,658 to 3,220,215
_3005
Dsui_3006: Dsui_3006 - (NiFe) hydrogenase assembly chaperone, HybE family, at 3,220,352 to 3,220,969
_3006
Dsui_3007: Dsui_3007 - hydrogenase assembly chaperone HypC/HupF, at 3,220,974 to 3,221,261
_3007
Dsui_3008: Dsui_3008 - hydrogenase expression/formation protein, at 3,221,261 to 3,221,761
_3008
Dsui_3009: Dsui_3009 - Ni,Fe-hydrogenase I large subunit, at 3,221,890 to 3,223,602
_3009
Position (kb)
3220
3221
3222 Strain fitness (log2 ratio)
-2
-1
0
1 at 3219.989 kb on - strand, within Dsui_3005 at 3220.047 kb on - strand, within Dsui_3005 at 3220.047 kb on - strand, within Dsui_3005 at 3220.127 kb on - strand, within Dsui_3005 at 3220.132 kb on - strand, within Dsui_3005 at 3220.186 kb on + strand at 3220.205 kb on + strand at 3220.417 kb on - strand, within Dsui_3006 at 3220.615 kb on - strand, within Dsui_3006 at 3221.107 kb on - strand, within Dsui_3007 at 3221.137 kb on + strand at 3221.246 kb on - strand at 3221.416 kb on - strand, within Dsui_3008 at 3221.445 kb on - strand, within Dsui_3008 at 3221.590 kb on - strand, within Dsui_3008 at 3221.616 kb on - strand, within Dsui_3008 at 3221.657 kb on + strand, within Dsui_3008 at 3221.700 kb on - strand at 3221.700 kb on - strand, within Dsui_3008 at 3221.707 kb on - strand, within Dsui_3008 at 3221.757 kb on - strand at 3221.953 kb on - strand at 3221.969 kb on - strand at 3222.079 kb on + strand at 3222.229 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 3,219,989 - Dsui_3005 0.59 -0.4 3,220,047 - Dsui_3005 0.70 -0.1 3,220,047 - Dsui_3005 0.70 -0.0 3,220,127 - Dsui_3005 0.84 -0.0 3,220,132 - Dsui_3005 0.85 +0.0 3,220,186 + +0.7 3,220,205 + -0.3 3,220,417 - Dsui_3006 0.11 -1.8 3,220,615 - Dsui_3006 0.43 -1.1 3,221,107 - Dsui_3007 0.46 -1.3 3,221,137 + -0.3 3,221,246 - +0.7 3,221,416 - Dsui_3008 0.31 +0.7 3,221,445 - Dsui_3008 0.37 -0.8 3,221,590 - Dsui_3008 0.66 +0.7 3,221,616 - Dsui_3008 0.71 -1.6 3,221,657 + Dsui_3008 0.79 -2.4 3,221,700 - -0.3 3,221,700 - Dsui_3008 0.88 +0.7 3,221,707 - Dsui_3008 0.89 -1.6 3,221,757 - +0.7 3,221,953 - +0.7 3,221,969 - +0.7 3,222,079 + +0.7 3,222,229 - +0.7
Or see this region's nucleotide sequence