Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1533 and Dsui_1534 are separated by 376 nucleotides Dsui_1534 and Dsui_1535 are separated by 30 nucleotides Dsui_1535 and Dsui_1536 are separated by 116 nucleotides Dsui_1536 and Dsui_1537 are separated by 120 nucleotides Dsui_1537 and Dsui_1538 are separated by 47 nucleotides
Dsui_1533: Dsui_1533 - disulfide bond formation protein DsbB, at 1,595,975 to 1,596,466
_1533
Dsui_1534: Dsui_1534 - ribosomal protein L33, at 1,596,843 to 1,596,998
_1534
Dsui_1535: Dsui_1535 - ribosomal protein L28, at 1,597,029 to 1,597,262
_1535
Dsui_1536: Dsui_1536 - PilZ domain-containing protein, at 1,597,379 to 1,597,705
_1536
Dsui_1537: Dsui_1537 - DNA repair protein radc, at 1,597,826 to 1,598,503
_1537
Dsui_1538: Dsui_1538 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase, at 1,598,551 to 1,599,735
_1538
Position (kb)
1597
1598 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1596.379 kb on - strand at 1596.525 kb on + strand at 1596.530 kb on + strand at 1596.838 kb on - strand at 1596.936 kb on - strand, within Dsui_1534 at 1597.013 kb on - strand at 1597.384 kb on - strand at 1597.384 kb on - strand at 1597.436 kb on - strand, within Dsui_1536 at 1597.443 kb on - strand, within Dsui_1536 at 1597.447 kb on - strand, within Dsui_1536 at 1597.497 kb on + strand, within Dsui_1536 at 1597.520 kb on - strand, within Dsui_1536 at 1597.597 kb on + strand, within Dsui_1536 at 1597.597 kb on + strand at 1597.597 kb on + strand, within Dsui_1536 at 1597.604 kb on + strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.605 kb on - strand, within Dsui_1536 at 1597.773 kb on + strand at 1597.788 kb on + strand at 1597.788 kb on + strand at 1597.796 kb on - strand at 1597.798 kb on - strand at 1597.798 kb on - strand at 1597.798 kb on - strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.824 kb on + strand at 1597.827 kb on - strand at 1597.827 kb on - strand at 1597.832 kb on - strand at 1597.832 kb on - strand at 1597.832 kb on - strand at 1597.837 kb on - strand at 1597.911 kb on - strand, within Dsui_1537 at 1597.917 kb on - strand at 1597.927 kb on + strand at 1597.969 kb on + strand at 1598.214 kb on + strand, within Dsui_1537 at 1598.253 kb on + strand, within Dsui_1537 at 1598.261 kb on - strand, within Dsui_1537 at 1598.277 kb on - strand, within Dsui_1537 at 1598.337 kb on - strand, within Dsui_1537 at 1598.377 kb on + strand at 1598.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 1,596,379 - +0.7 1,596,525 + +0.1 1,596,530 + +0.7 1,596,838 - -0.2 1,596,936 - Dsui_1534 0.60 -0.8 1,597,013 - -0.8 1,597,384 - -0.3 1,597,384 - -0.6 1,597,436 - Dsui_1536 0.17 -0.5 1,597,443 - Dsui_1536 0.20 -0.1 1,597,447 - Dsui_1536 0.21 -0.3 1,597,497 + Dsui_1536 0.36 -1.3 1,597,520 - Dsui_1536 0.43 -2.1 1,597,597 + Dsui_1536 0.67 -0.3 1,597,597 + -0.3 1,597,597 + Dsui_1536 0.67 -3.0 1,597,604 + Dsui_1536 0.69 -0.9 1,597,605 - Dsui_1536 0.69 -1.7 1,597,605 - Dsui_1536 0.69 +0.2 1,597,605 - Dsui_1536 0.69 -1.4 1,597,605 - Dsui_1536 0.69 -1.2 1,597,605 - Dsui_1536 0.69 +0.7 1,597,773 + +0.7 1,597,788 + -0.9 1,597,788 + -1.9 1,597,796 - +0.5 1,597,798 - +0.7 1,597,798 - -1.9 1,597,798 - -0.9 1,597,824 + +0.7 1,597,824 + -2.1 1,597,824 + -0.3 1,597,824 + +0.7 1,597,824 + +0.7 1,597,824 + -2.1 1,597,827 - -0.3 1,597,827 - -1.9 1,597,832 - -0.3 1,597,832 - -0.9 1,597,832 - +0.7 1,597,837 - +0.7 1,597,911 - Dsui_1537 0.13 -1.3 1,597,917 - +0.7 1,597,927 + +0.7 1,597,969 + +0.7 1,598,214 + Dsui_1537 0.57 -0.3 1,598,253 + Dsui_1537 0.63 -0.3 1,598,261 - Dsui_1537 0.64 +0.7 1,598,277 - Dsui_1537 0.67 -0.9 1,598,337 - Dsui_1537 0.75 -1.3 1,598,377 + +0.7 1,598,511 + -0.1
Or see this region's nucleotide sequence