Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1423 and Dsui_1424 are separated by 8 nucleotides Dsui_1424 and Dsui_1425 are separated by 69 nucleotides Dsui_1425 and Dsui_1426 are separated by 23 nucleotides
Dsui_1423: Dsui_1423 - twin arginine-targeting protein translocase TatB, at 1,476,075 to 1,476,458
_1423
Dsui_1424: Dsui_1424 - twin arginine targeting protein translocase subunit TatC, at 1,476,467 to 1,477,342
_1424
Dsui_1425: Dsui_1425 - putative glutamine amidotransferase, at 1,477,412 to 1,478,236
_1425
Dsui_1426: Dsui_1426 - periplasmic serine protease, Do/DeqQ family, at 1,478,260 to 1,479,414
_1426
Position (kb)
1477
1478
1479 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1477.307 kb on + strand at 1477.514 kb on + strand at 1477.514 kb on + strand, within Dsui_1425 at 1477.542 kb on + strand, within Dsui_1425 at 1477.626 kb on + strand, within Dsui_1425 at 1477.680 kb on - strand, within Dsui_1425 at 1477.682 kb on - strand, within Dsui_1425 at 1477.965 kb on + strand, within Dsui_1425 at 1477.998 kb on - strand, within Dsui_1425 at 1478.058 kb on + strand, within Dsui_1425 at 1478.069 kb on + strand, within Dsui_1425 at 1478.410 kb on - strand, within Dsui_1426 at 1478.420 kb on - strand, within Dsui_1426 at 1478.517 kb on + strand, within Dsui_1426 at 1478.747 kb on + strand, within Dsui_1426 at 1478.801 kb on - strand, within Dsui_1426 at 1478.846 kb on + strand at 1478.938 kb on - strand, within Dsui_1426 at 1479.096 kb on + strand, within Dsui_1426 at 1479.096 kb on + strand, within Dsui_1426 at 1479.104 kb on - strand, within Dsui_1426 at 1479.214 kb on - strand, within Dsui_1426 at 1479.215 kb on + strand, within Dsui_1426
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 1,477,307 + -0.4 1,477,514 + +0.6 1,477,514 + Dsui_1425 0.12 -2.5 1,477,542 + Dsui_1425 0.16 -1.1 1,477,626 + Dsui_1425 0.26 -3.5 1,477,680 - Dsui_1425 0.32 -0.3 1,477,682 - Dsui_1425 0.33 -0.6 1,477,965 + Dsui_1425 0.67 -0.4 1,477,998 - Dsui_1425 0.71 -0.3 1,478,058 + Dsui_1425 0.78 -1.0 1,478,069 + Dsui_1425 0.80 +0.6 1,478,410 - Dsui_1426 0.13 -0.7 1,478,420 - Dsui_1426 0.14 -0.4 1,478,517 + Dsui_1426 0.22 -0.4 1,478,747 + Dsui_1426 0.42 -0.4 1,478,801 - Dsui_1426 0.47 -3.0 1,478,846 + +0.6 1,478,938 - Dsui_1426 0.59 -0.8 1,479,096 + Dsui_1426 0.72 -1.0 1,479,096 + Dsui_1426 0.72 -0.4 1,479,104 - Dsui_1426 0.73 -0.8 1,479,214 - Dsui_1426 0.83 -0.6 1,479,215 + Dsui_1426 0.83 -1.4
Or see this region's nucleotide sequence