Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1011 and Dsui_1012 are separated by 135 nucleotides Dsui_1012 and Dsui_1013 are separated by 18 nucleotides Dsui_1013 and Dsui_1014 are separated by 69 nucleotides
Dsui_1011: Dsui_1011 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,041,420 to 1,044,800
_1011
Dsui_1012: Dsui_1012 - ABC-type branched-chain amino acid transport system, periplasmic component, at 1,044,936 to 1,046,174
_1012
Dsui_1013: Dsui_1013 - thiamine biosynthesis protein ThiS, at 1,046,193 to 1,046,402
_1013
Dsui_1014: Dsui_1014 - putative enzyme of thiazole biosynthesis, at 1,046,472 to 1,047,257
_1014
Position (kb)
1044
1045
1046
1047 Strain fitness (log2 ratio)
-2
-1
0
1 at 1043.967 kb on - strand, within Dsui_1011 at 1043.967 kb on - strand, within Dsui_1011 at 1043.992 kb on - strand, within Dsui_1011 at 1044.106 kb on + strand, within Dsui_1011 at 1044.226 kb on - strand, within Dsui_1011 at 1044.374 kb on + strand, within Dsui_1011 at 1044.534 kb on - strand at 1044.590 kb on + strand at 1044.721 kb on + strand at 1044.739 kb on - strand at 1044.818 kb on - strand at 1044.939 kb on + strand at 1044.939 kb on - strand at 1044.947 kb on - strand at 1044.973 kb on - strand at 1045.061 kb on + strand, within Dsui_1012 at 1045.097 kb on - strand, within Dsui_1012 at 1045.267 kb on - strand, within Dsui_1012 at 1045.689 kb on - strand, within Dsui_1012 at 1045.749 kb on - strand, within Dsui_1012 at 1045.749 kb on - strand, within Dsui_1012 at 1045.825 kb on + strand at 1045.871 kb on - strand, within Dsui_1012 at 1045.877 kb on + strand at 1045.885 kb on - strand, within Dsui_1012 at 1045.902 kb on + strand, within Dsui_1012 at 1045.910 kb on - strand, within Dsui_1012 at 1045.948 kb on + strand at 1045.963 kb on + strand at 1045.963 kb on + strand at 1045.992 kb on + strand at 1046.019 kb on + strand, within Dsui_1012 at 1046.022 kb on - strand, within Dsui_1012 at 1046.032 kb on - strand, within Dsui_1012 at 1046.151 kb on + strand at 1046.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 1,043,967 - Dsui_1011 0.75 +0.3 1,043,967 - Dsui_1011 0.75 -0.3 1,043,992 - Dsui_1011 0.76 -0.2 1,044,106 + Dsui_1011 0.79 -1.2 1,044,226 - Dsui_1011 0.83 -0.8 1,044,374 + Dsui_1011 0.87 -1.2 1,044,534 - +0.1 1,044,590 + -0.2 1,044,721 + +0.8 1,044,739 - -0.3 1,044,818 - -0.2 1,044,939 + -2.0 1,044,939 - -1.8 1,044,947 - +0.3 1,044,973 - +1.1 1,045,061 + Dsui_1012 0.10 +0.8 1,045,097 - Dsui_1012 0.13 -0.5 1,045,267 - Dsui_1012 0.27 -1.0 1,045,689 - Dsui_1012 0.61 -0.2 1,045,749 - Dsui_1012 0.66 -1.5 1,045,749 - Dsui_1012 0.66 -0.0 1,045,825 + -0.2 1,045,871 - Dsui_1012 0.75 +0.8 1,045,877 + +0.8 1,045,885 - Dsui_1012 0.77 +0.6 1,045,902 + Dsui_1012 0.78 -0.8 1,045,910 - Dsui_1012 0.79 -0.1 1,045,948 + +0.8 1,045,963 + +0.8 1,045,963 + +0.8 1,045,992 + +0.8 1,046,019 + Dsui_1012 0.87 -1.2 1,046,022 - Dsui_1012 0.88 -1.2 1,046,032 - Dsui_1012 0.88 -0.2 1,046,151 + -0.2 1,046,152 + -2.4
Or see this region's nucleotide sequence