Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1000 and Dsui_1001 overlap by 4 nucleotides Dsui_1001 and Dsui_1002 overlap by 4 nucleotides Dsui_1002 and Dsui_1003 are separated by 15 nucleotides
Dsui_1000: Dsui_1000 - hypothetical protein, at 1,027,655 to 1,028,968
_1000
Dsui_1001: Dsui_1001 - hypothetical protein, at 1,028,965 to 1,030,335
_1001
Dsui_1002: Dsui_1002 - prolyl oligopeptidase family protein, at 1,030,332 to 1,031,246
_1002
Dsui_1003: Dsui_1003 - phosphoribosylglycinamide formyltransferase 2, at 1,031,262 to 1,032,494
_1003
Position (kb)
1028
1029
1030
1031 Strain fitness (log2 ratio)
-2
-1
0
1 at 1028.249 kb on - strand at 1028.431 kb on - strand, within Dsui_1000 at 1028.441 kb on - strand at 1028.450 kb on + strand at 1028.577 kb on - strand at 1028.602 kb on - strand at 1028.817 kb on + strand, within Dsui_1000 at 1029.089 kb on + strand at 1029.120 kb on - strand, within Dsui_1001 at 1029.130 kb on - strand at 1029.198 kb on + strand, within Dsui_1001 at 1029.203 kb on + strand at 1029.206 kb on - strand, within Dsui_1001 at 1029.216 kb on - strand, within Dsui_1001 at 1029.260 kb on + strand at 1029.339 kb on + strand, within Dsui_1001 at 1029.378 kb on - strand at 1029.389 kb on + strand, within Dsui_1001 at 1029.402 kb on - strand, within Dsui_1001 at 1029.406 kb on + strand, within Dsui_1001 at 1029.475 kb on + strand, within Dsui_1001 at 1029.543 kb on + strand, within Dsui_1001 at 1029.575 kb on - strand, within Dsui_1001 at 1029.599 kb on - strand, within Dsui_1001 at 1029.865 kb on + strand, within Dsui_1001 at 1029.893 kb on + strand, within Dsui_1001 at 1029.968 kb on - strand, within Dsui_1001 at 1030.127 kb on - strand at 1030.175 kb on + strand, within Dsui_1001 at 1030.191 kb on + strand at 1030.204 kb on - strand at 1030.204 kb on - strand at 1030.278 kb on + strand at 1030.298 kb on + strand at 1030.358 kb on - strand at 1030.552 kb on + strand, within Dsui_1002 at 1030.631 kb on + strand at 1030.649 kb on - strand, within Dsui_1002 at 1030.958 kb on - strand, within Dsui_1002 at 1031.172 kb on + strand at 1031.175 kb on - strand at 1031.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 1,028,249 - -0.2 1,028,431 - Dsui_1000 0.59 -0.8 1,028,441 - +0.8 1,028,450 + +0.8 1,028,577 - +0.8 1,028,602 - +0.8 1,028,817 + Dsui_1000 0.88 +0.8 1,029,089 + +0.8 1,029,120 - Dsui_1001 0.11 -1.8 1,029,130 - +0.8 1,029,198 + Dsui_1001 0.17 -1.2 1,029,203 + +0.8 1,029,206 - Dsui_1001 0.18 -0.2 1,029,216 - Dsui_1001 0.18 -1.5 1,029,260 + +0.8 1,029,339 + Dsui_1001 0.27 -0.5 1,029,378 - +0.8 1,029,389 + Dsui_1001 0.31 -0.8 1,029,402 - Dsui_1001 0.32 +0.8 1,029,406 + Dsui_1001 0.32 -1.5 1,029,475 + Dsui_1001 0.37 -1.2 1,029,543 + Dsui_1001 0.42 -0.8 1,029,575 - Dsui_1001 0.44 +0.8 1,029,599 - Dsui_1001 0.46 -0.2 1,029,865 + Dsui_1001 0.66 +0.8 1,029,893 + Dsui_1001 0.68 +0.8 1,029,968 - Dsui_1001 0.73 -0.2 1,030,127 - +0.8 1,030,175 + Dsui_1001 0.88 -0.8 1,030,191 + +0.8 1,030,204 - -1.2 1,030,204 - -1.8 1,030,278 + -1.8 1,030,298 + -0.8 1,030,358 - +0.8 1,030,552 + Dsui_1002 0.24 -1.2 1,030,631 + -0.8 1,030,649 - Dsui_1002 0.35 -0.8 1,030,958 - Dsui_1002 0.68 -1.0 1,031,172 + -1.2 1,031,175 - -2.0 1,031,180 - -0.4
Or see this region's nucleotide sequence