Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0980 and Dsui_0981 are separated by 20 nucleotides Dsui_0981 and Dsui_0982 are separated by 63 nucleotides Dsui_0982 and Dsui_0983 are separated by 188 nucleotides
Dsui_0980: Dsui_0980 - acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta), at 1,006,300 to 1,007,907
_0980
Dsui_0981: Dsui_0981 - enoyl-CoA hydratase/carnithine racemase, at 1,007,928 to 1,008,719
_0981
Dsui_0982: Dsui_0982 - acetyl/propionyl-CoA carboxylase, alpha subunit, at 1,008,783 to 1,010,786
_0982
Dsui_0983: Dsui_0983 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,010,975 to 1,013,275
_0983
Position (kb)
1008
1009
1010
1011 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1007.790 kb on + strand at 1008.053 kb on + strand, within Dsui_0981 at 1008.084 kb on - strand, within Dsui_0981 at 1008.613 kb on + strand, within Dsui_0981 at 1008.637 kb on - strand, within Dsui_0981 at 1008.927 kb on + strand at 1009.031 kb on - strand, within Dsui_0982 at 1009.295 kb on + strand at 1009.318 kb on - strand at 1009.385 kb on + strand, within Dsui_0982 at 1009.476 kb on - strand, within Dsui_0982 at 1009.486 kb on + strand, within Dsui_0982 at 1009.646 kb on + strand, within Dsui_0982 at 1009.703 kb on - strand at 1009.704 kb on - strand, within Dsui_0982 at 1009.777 kb on + strand, within Dsui_0982 at 1009.804 kb on - strand, within Dsui_0982 at 1009.873 kb on + strand, within Dsui_0982 at 1009.929 kb on - strand, within Dsui_0982 at 1009.978 kb on - strand, within Dsui_0982 at 1010.003 kb on - strand, within Dsui_0982 at 1010.083 kb on + strand, within Dsui_0982 at 1010.390 kb on + strand, within Dsui_0982 at 1010.537 kb on - strand, within Dsui_0982 at 1010.615 kb on + strand at 1010.686 kb on + strand at 1010.754 kb on - strand at 1010.780 kb on + strand at 1010.863 kb on + strand at 1010.876 kb on - strand at 1011.031 kb on + strand at 1011.114 kb on + strand at 1011.132 kb on + strand at 1011.132 kb on + strand at 1011.369 kb on - strand, within Dsui_0983 at 1011.385 kb on + strand, within Dsui_0983 at 1011.488 kb on - strand, within Dsui_0983 at 1011.507 kb on + strand, within Dsui_0983 at 1011.517 kb on + strand, within Dsui_0983 at 1011.635 kb on - strand, within Dsui_0983 at 1011.642 kb on + strand, within Dsui_0983 at 1011.706 kb on + strand, within Dsui_0983 at 1011.767 kb on - strand, within Dsui_0983
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 1,007,790 + -0.8 1,008,053 + Dsui_0981 0.16 +0.5 1,008,084 - Dsui_0981 0.20 -2.0 1,008,613 + Dsui_0981 0.86 -0.8 1,008,637 - Dsui_0981 0.90 -0.2 1,008,927 + -1.2 1,009,031 - Dsui_0982 0.12 -1.2 1,009,295 + -0.8 1,009,318 - +0.8 1,009,385 + Dsui_0982 0.30 +0.3 1,009,476 - Dsui_0982 0.35 -0.2 1,009,486 + Dsui_0982 0.35 +0.8 1,009,646 + Dsui_0982 0.43 +0.1 1,009,703 - +0.8 1,009,704 - Dsui_0982 0.46 -1.2 1,009,777 + Dsui_0982 0.50 -0.7 1,009,804 - Dsui_0982 0.51 -0.8 1,009,873 + Dsui_0982 0.54 -0.4 1,009,929 - Dsui_0982 0.57 -0.8 1,009,978 - Dsui_0982 0.60 -0.2 1,010,003 - Dsui_0982 0.61 -1.2 1,010,083 + Dsui_0982 0.65 -0.9 1,010,390 + Dsui_0982 0.80 -0.8 1,010,537 - Dsui_0982 0.88 -0.6 1,010,615 + -0.6 1,010,686 + -0.4 1,010,754 - +0.8 1,010,780 + -0.8 1,010,863 + +0.8 1,010,876 - -0.8 1,011,031 + +0.0 1,011,114 + +0.6 1,011,132 + -0.7 1,011,132 + +1.8 1,011,369 - Dsui_0983 0.17 -0.8 1,011,385 + Dsui_0983 0.18 -0.8 1,011,488 - Dsui_0983 0.22 -0.2 1,011,507 + Dsui_0983 0.23 -0.5 1,011,517 + Dsui_0983 0.24 -0.6 1,011,635 - Dsui_0983 0.29 +0.8 1,011,642 + Dsui_0983 0.29 -1.4 1,011,706 + Dsui_0983 0.32 -0.8 1,011,767 - Dsui_0983 0.34 -0.8
Or see this region's nucleotide sequence