Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0649 and Dsui_0650 overlap by 4 nucleotides Dsui_0650 and Dsui_0651 are separated by 14 nucleotides Dsui_0651 and Dsui_0652 are separated by 4 nucleotides
Dsui_0649: Dsui_0649 - diaminopimelate epimerase, at 642,773 to 643,603
_0649
Dsui_0650: Dsui_0650 - hypothetical protein, at 643,600 to 644,280
_0650
Dsui_0651: Dsui_0651 - tyrosine recombinase XerC, at 644,295 to 645,200
_0651
Dsui_0652: Dsui_0652 - putative membrane protein, at 645,205 to 646,335
_0652
Position (kb) 644
645
646 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 643.608 kb on + strand at 643.616 kb on - strand at 643.650 kb on - strand at 643.676 kb on - strand, within Dsui_0650 at 643.725 kb on + strand, within Dsui_0650 at 643.757 kb on + strand, within Dsui_0650 at 643.776 kb on + strand, within Dsui_0650 at 643.784 kb on - strand at 643.784 kb on - strand, within Dsui_0650 at 643.864 kb on - strand, within Dsui_0650 at 644.038 kb on + strand, within Dsui_0650 at 644.041 kb on - strand, within Dsui_0650 at 644.047 kb on - strand, within Dsui_0650 at 644.136 kb on - strand at 644.282 kb on - strand at 644.428 kb on + strand, within Dsui_0651 at 644.436 kb on - strand, within Dsui_0651 at 644.436 kb on - strand, within Dsui_0651 at 644.632 kb on - strand at 644.731 kb on - strand at 644.739 kb on - strand, within Dsui_0651 at 644.890 kb on + strand, within Dsui_0651 at 644.999 kb on - strand, within Dsui_0651 at 645.057 kb on + strand, within Dsui_0651 at 645.133 kb on - strand at 645.133 kb on - strand at 645.133 kb on - strand at 645.162 kb on + strand at 645.170 kb on - strand at 645.196 kb on + strand at 645.215 kb on - strand at 645.229 kb on + strand at 645.519 kb on - strand, within Dsui_0652 at 645.722 kb on + strand, within Dsui_0652 at 645.727 kb on + strand, within Dsui_0652 at 645.736 kb on - strand at 645.747 kb on + strand, within Dsui_0652 at 645.790 kb on + strand, within Dsui_0652 at 645.855 kb on - strand at 645.855 kb on - strand at 645.931 kb on + strand, within Dsui_0652 at 645.951 kb on + strand, within Dsui_0652 at 645.957 kb on + strand, within Dsui_0652 at 645.965 kb on + strand, within Dsui_0652 at 646.000 kb on + strand, within Dsui_0652 at 646.045 kb on - strand at 646.138 kb on - strand, within Dsui_0652
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 643,608 + +0.2 643,616 - +0.8 643,650 - +0.8 643,676 - Dsui_0650 0.11 -0.2 643,725 + Dsui_0650 0.18 -0.6 643,757 + Dsui_0650 0.23 -0.9 643,776 + Dsui_0650 0.26 -1.9 643,784 - +0.8 643,784 - Dsui_0650 0.27 +0.8 643,864 - Dsui_0650 0.39 +0.8 644,038 + Dsui_0650 0.64 -0.8 644,041 - Dsui_0650 0.65 +0.8 644,047 - Dsui_0650 0.66 +0.8 644,136 - +0.8 644,282 - -2.5 644,428 + Dsui_0651 0.15 -0.4 644,436 - Dsui_0651 0.16 -0.2 644,436 - Dsui_0651 0.16 -0.2 644,632 - +0.8 644,731 - +0.8 644,739 - Dsui_0651 0.49 -1.2 644,890 + Dsui_0651 0.66 -2.3 644,999 - Dsui_0651 0.78 -0.2 645,057 + Dsui_0651 0.84 -1.0 645,133 - +0.8 645,133 - +0.8 645,133 - -0.2 645,162 + -2.9 645,170 - -0.8 645,196 + -0.4 645,215 - -1.2 645,229 + +0.2 645,519 - Dsui_0652 0.28 -0.2 645,722 + Dsui_0652 0.46 -1.2 645,727 + Dsui_0652 0.46 -1.2 645,736 - +0.8 645,747 + Dsui_0652 0.48 -2.2 645,790 + Dsui_0652 0.52 -0.5 645,855 - +0.8 645,855 - +0.8 645,931 + Dsui_0652 0.64 -3.2 645,951 + Dsui_0652 0.66 +0.2 645,957 + Dsui_0652 0.66 +0.2 645,965 + Dsui_0652 0.67 -0.2 646,000 + Dsui_0652 0.70 -0.2 646,045 - +0.8 646,138 - Dsui_0652 0.82 -0.8
Or see this region's nucleotide sequence