Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0422 and Dsui_0423 are separated by 50 nucleotides Dsui_0423 and Dsui_0424 are separated by 213 nucleotides
Dsui_0422: Dsui_0422 - thiamine biosynthesis protein ThiC, at 424,450 to 426,366
_0422
Dsui_0423: Dsui_0423 - putative permease, at 426,417 to 427,475
_0423
Dsui_0424: Dsui_0424 - phosphoketolase, at 427,689 to 430,109
_0424
Position (kb) 426
427
428 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 426.433 kb on + strand at 426.496 kb on - strand at 426.566 kb on - strand, within Dsui_0423 at 426.596 kb on + strand, within Dsui_0423 at 426.691 kb on - strand, within Dsui_0423 at 426.700 kb on + strand at 426.737 kb on + strand at 426.750 kb on - strand, within Dsui_0423 at 426.753 kb on + strand, within Dsui_0423 at 426.756 kb on - strand, within Dsui_0423 at 426.845 kb on - strand, within Dsui_0423 at 426.850 kb on - strand, within Dsui_0423 at 426.917 kb on - strand, within Dsui_0423 at 427.006 kb on - strand, within Dsui_0423 at 427.067 kb on - strand, within Dsui_0423 at 427.091 kb on + strand, within Dsui_0423 at 427.184 kb on - strand, within Dsui_0423 at 427.282 kb on - strand, within Dsui_0423 at 427.543 kb on + strand at 427.543 kb on + strand at 427.828 kb on + strand at 427.908 kb on + strand at 427.931 kb on - strand, within Dsui_0424 at 428.319 kb on - strand, within Dsui_0424 at 428.324 kb on - strand, within Dsui_0424 at 428.341 kb on - strand at 428.454 kb on + strand, within Dsui_0424 at 428.459 kb on + strand, within Dsui_0424
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 426,433 + -2.5 426,496 - -2.1 426,566 - Dsui_0423 0.14 -4.9 426,596 + Dsui_0423 0.17 -0.9 426,691 - Dsui_0423 0.26 -3.2 426,700 + -0.3 426,737 + +0.7 426,750 - Dsui_0423 0.31 -5.6 426,753 + Dsui_0423 0.32 -1.9 426,756 - Dsui_0423 0.32 -5.2 426,845 - Dsui_0423 0.40 -6.6 426,850 - Dsui_0423 0.41 -3.6 426,917 - Dsui_0423 0.47 -4.9 427,006 - Dsui_0423 0.56 -3.6 427,067 - Dsui_0423 0.61 -3.1 427,091 + Dsui_0423 0.64 -0.9 427,184 - Dsui_0423 0.72 -3.9 427,282 - Dsui_0423 0.82 -3.8 427,543 + +0.7 427,543 + -0.9 427,828 + -0.5 427,908 + +0.2 427,931 - Dsui_0424 0.10 -1.3 428,319 - Dsui_0424 0.26 -0.9 428,324 - Dsui_0424 0.26 -0.3 428,341 - +0.7 428,454 + Dsui_0424 0.32 -0.2 428,459 + Dsui_0424 0.32 +0.4
Or see this region's nucleotide sequence