Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0421 and Dsui_0422 are separated by 330 nucleotides Dsui_0422 and Dsui_0423 are separated by 50 nucleotides
Dsui_0421: Dsui_0421 - carbonic anhydrase, at 422,683 to 424,119
_0421
Dsui_0422: Dsui_0422 - thiamine biosynthesis protein ThiC, at 424,450 to 426,366
_0422
Dsui_0423: Dsui_0423 - putative permease, at 426,417 to 427,475
_0423
Position (kb) 424
425
426
427 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 423.463 kb on + strand at 423.625 kb on - strand, within Dsui_0421 at 423.647 kb on + strand, within Dsui_0421 at 423.769 kb on - strand, within Dsui_0421 at 423.857 kb on + strand, within Dsui_0421 at 423.897 kb on - strand, within Dsui_0421 at 423.935 kb on + strand, within Dsui_0421 at 423.985 kb on - strand at 424.115 kb on + strand at 424.115 kb on + strand at 424.123 kb on - strand at 424.140 kb on + strand at 424.140 kb on + strand at 424.151 kb on + strand at 424.151 kb on + strand at 424.159 kb on - strand at 424.159 kb on - strand at 424.159 kb on - strand at 424.181 kb on + strand at 424.192 kb on + strand at 424.242 kb on + strand at 424.242 kb on + strand at 424.250 kb on - strand at 424.369 kb on + strand at 426.433 kb on + strand at 426.496 kb on - strand at 426.566 kb on - strand, within Dsui_0423 at 426.596 kb on + strand, within Dsui_0423 at 426.691 kb on - strand, within Dsui_0423 at 426.700 kb on + strand at 426.737 kb on + strand at 426.750 kb on - strand, within Dsui_0423 at 426.753 kb on + strand, within Dsui_0423 at 426.756 kb on - strand, within Dsui_0423 at 426.845 kb on - strand, within Dsui_0423 at 426.850 kb on - strand, within Dsui_0423 at 426.917 kb on - strand, within Dsui_0423 at 427.006 kb on - strand, within Dsui_0423 at 427.067 kb on - strand, within Dsui_0423 at 427.091 kb on + strand, within Dsui_0423 at 427.184 kb on - strand, within Dsui_0423 at 427.282 kb on - strand, within Dsui_0423
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 423,463 + +0.7 423,625 - Dsui_0421 0.66 -0.9 423,647 + Dsui_0421 0.67 -0.3 423,769 - Dsui_0421 0.76 +0.7 423,857 + Dsui_0421 0.82 -0.3 423,897 - Dsui_0421 0.84 +0.7 423,935 + Dsui_0421 0.87 -0.9 423,985 - -1.9 424,115 + -0.3 424,115 + -0.9 424,123 - -0.9 424,140 + -1.9 424,140 + -0.3 424,151 + +0.7 424,151 + +0.7 424,159 - -0.3 424,159 - +0.7 424,159 - -0.9 424,181 + +0.7 424,192 + +0.7 424,242 + -0.3 424,242 + -0.8 424,250 - -0.3 424,369 + -1.6 426,433 + -2.5 426,496 - -2.1 426,566 - Dsui_0423 0.14 -4.9 426,596 + Dsui_0423 0.17 -0.9 426,691 - Dsui_0423 0.26 -3.2 426,700 + -0.3 426,737 + +0.7 426,750 - Dsui_0423 0.31 -5.6 426,753 + Dsui_0423 0.32 -1.9 426,756 - Dsui_0423 0.32 -5.2 426,845 - Dsui_0423 0.40 -6.6 426,850 - Dsui_0423 0.41 -3.6 426,917 - Dsui_0423 0.47 -4.9 427,006 - Dsui_0423 0.56 -3.6 427,067 - Dsui_0423 0.61 -3.1 427,091 + Dsui_0423 0.64 -0.9 427,184 - Dsui_0423 0.72 -3.9 427,282 - Dsui_0423 0.82 -3.8
Or see this region's nucleotide sequence