Experiment: ALP with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0384 and Dsui_0385 are separated by 52 nucleotides Dsui_0385 and Dsui_0386 overlap by 4 nucleotides Dsui_0386 and Dsui_0387 are separated by 55 nucleotides Dsui_0387 and Dsui_0388 are separated by 88 nucleotides
Dsui_0384: Dsui_0384 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 383,964 to 385,313
_0384
Dsui_0385: Dsui_0385 - hypothetical protein, at 385,366 to 385,977
_0385
Dsui_0386: Dsui_0386 - thiol-disulfide isomerase-like thioredoxin, at 385,974 to 386,522
_0386
Dsui_0387: Dsui_0387 - 3-dehydroquinate dehydratase, type II, at 386,578 to 387,078
_0387
Dsui_0388: Dsui_0388 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 387,167 to 387,616
_0388
Position (kb)
385
386
387 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 385.040 kb on - strand, within Dsui_0384 at 385.055 kb on - strand, within Dsui_0384 at 385.257 kb on - strand at 385.262 kb on - strand at 385.270 kb on - strand at 385.281 kb on - strand at 385.282 kb on + strand at 385.328 kb on + strand at 385.351 kb on - strand at 385.360 kb on - strand at 385.369 kb on + strand at 385.371 kb on - strand at 385.372 kb on - strand at 385.736 kb on + strand, within Dsui_0385 at 385.745 kb on + strand, within Dsui_0385 at 385.745 kb on + strand, within Dsui_0385 at 385.754 kb on - strand at 385.787 kb on + strand, within Dsui_0385 at 385.787 kb on + strand, within Dsui_0385 at 385.856 kb on + strand, within Dsui_0385 at 385.856 kb on + strand, within Dsui_0385 at 385.864 kb on + strand, within Dsui_0385 at 385.869 kb on + strand, within Dsui_0385 at 385.877 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.963 kb on - strand at 386.139 kb on + strand, within Dsui_0386 at 386.151 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.178 kb on - strand at 386.180 kb on + strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.229 kb on - strand, within Dsui_0386 at 386.280 kb on - strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.303 kb on - strand, within Dsui_0386 at 386.428 kb on + strand, within Dsui_0386 at 386.461 kb on - strand, within Dsui_0386 at 386.570 kb on - strand at 386.575 kb on + strand at 386.613 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.134 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Dimethyl Sulfoxide 2 vol% remove 385,040 - Dsui_0384 0.80 +0.1 385,055 - Dsui_0384 0.81 -1.3 385,257 - -0.4 385,262 - -1.2 385,270 - -1.3 385,281 - -0.9 385,282 + -0.3 385,328 + -0.6 385,351 - -0.4 385,360 - +0.7 385,369 + -1.5 385,371 - +0.7 385,372 - +0.7 385,736 + Dsui_0385 0.60 -0.9 385,745 + Dsui_0385 0.62 -1.6 385,745 + Dsui_0385 0.62 -1.2 385,754 - +0.7 385,787 + Dsui_0385 0.69 -1.6 385,787 + Dsui_0385 0.69 +0.7 385,856 + Dsui_0385 0.80 -1.3 385,856 + Dsui_0385 0.80 -1.0 385,864 + Dsui_0385 0.81 +0.2 385,869 + Dsui_0385 0.82 -0.2 385,877 - Dsui_0385 0.83 -1.6 385,887 - Dsui_0385 0.85 +0.4 385,887 - Dsui_0385 0.85 -1.3 385,887 - Dsui_0385 0.85 -0.9 385,963 - -1.3 386,139 + Dsui_0386 0.30 +0.7 386,151 + Dsui_0386 0.32 -0.6 386,170 + Dsui_0386 0.36 -2.0 386,170 + Dsui_0386 0.36 -0.3 386,178 - +0.7 386,180 + Dsui_0386 0.38 -1.2 386,214 - Dsui_0386 0.44 +0.7 386,214 - Dsui_0386 0.44 -1.9 386,229 - Dsui_0386 0.46 -0.3 386,280 - Dsui_0386 0.56 -1.6 386,295 + Dsui_0386 0.58 -0.5 386,295 + Dsui_0386 0.58 -0.9 386,303 - Dsui_0386 0.60 +0.7 386,428 + Dsui_0386 0.83 -0.9 386,461 - Dsui_0386 0.89 +0.7 386,570 - -1.0 386,575 + -0.8 386,613 + -0.6 387,086 + -0.3 387,086 + +0.0 387,086 + -0.7 387,134 + -2.9
Or see this region's nucleotide sequence