Experiment: BHIS with Zinc Pyrithione 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0544 and BT0545 are separated by 82 nucleotides BT0545 and BT0546 are separated by 151 nucleotides BT0546 and BT0547 are separated by 74 nucleotides
BT0544: BT0544 - ammonium transporter (NCBI ptt file), at 670,232 to 671,689
BT0544
BT0545: BT0545 - nitrogen regulatory protein P-II (NCBI ptt file), at 671,772 to 672,128
BT0545
BT0546: BT0546 - conserved hypothetical protein (NCBI ptt file), at 672,280 to 673,005
BT0546
BT0547: BT0547 - aspartate aminotransferase (NCBI ptt file), at 673,080 to 674,312
BT0547
Position (kb)
671
672
673 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 670.835 kb on + strand, within BT0544 at 670.924 kb on + strand, within BT0544 at 670.925 kb on - strand, within BT0544 at 671.099 kb on - strand, within BT0544 at 671.104 kb on + strand, within BT0544 at 671.105 kb on - strand, within BT0544 at 671.107 kb on + strand, within BT0544 at 671.110 kb on - strand, within BT0544 at 671.118 kb on + strand, within BT0544 at 671.548 kb on + strand at 671.636 kb on - strand at 671.696 kb on + strand at 671.697 kb on - strand at 671.938 kb on - strand, within BT0545 at 671.945 kb on + strand, within BT0545 at 672.000 kb on - strand, within BT0545 at 672.168 kb on + strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.227 kb on + strand at 672.248 kb on + strand at 672.306 kb on - strand at 672.318 kb on + strand at 672.329 kb on - strand at 672.472 kb on + strand, within BT0546 at 672.473 kb on - strand, within BT0546 at 672.531 kb on - strand, within BT0546 at 672.631 kb on + strand, within BT0546 at 672.656 kb on + strand, within BT0546 at 672.759 kb on + strand, within BT0546 at 672.815 kb on + strand, within BT0546 at 672.827 kb on - strand, within BT0546 at 672.873 kb on + strand, within BT0546 at 672.910 kb on + strand, within BT0546 at 673.058 kb on + strand at 673.059 kb on - strand at 673.078 kb on + strand at 673.115 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Zinc Pyrithione 0.05 mM remove 670,835 + BT0544 0.41 -0.6 670,924 + BT0544 0.47 -1.8 670,925 - BT0544 0.48 -1.0 671,099 - BT0544 0.59 +0.4 671,104 + BT0544 0.60 +3.0 671,105 - BT0544 0.60 -2.1 671,107 + BT0544 0.60 +0.6 671,110 - BT0544 0.60 -1.3 671,118 + BT0544 0.61 -1.0 671,548 + +2.0 671,636 - -0.6 671,696 + -2.5 671,697 - -0.6 671,938 - BT0545 0.46 -2.7 671,945 + BT0545 0.48 -2.6 672,000 - BT0545 0.64 -2.3 672,168 + -1.6 672,201 - +1.0 672,201 - -2.2 672,201 - -1.9 672,227 + +0.2 672,248 + -0.6 672,306 - -0.6 672,318 + -0.6 672,329 - +0.0 672,472 + BT0546 0.26 +1.0 672,473 - BT0546 0.27 -0.2 672,531 - BT0546 0.35 +0.0 672,631 + BT0546 0.48 +0.3 672,656 + BT0546 0.52 -0.6 672,759 + BT0546 0.66 +0.3 672,815 + BT0546 0.74 +2.0 672,827 - BT0546 0.75 +0.4 672,873 + BT0546 0.82 -0.7 672,910 + BT0546 0.87 -0.9 673,058 + +0.0 673,059 - -0.8 673,078 + +1.4 673,115 + +0.0
Or see this region's nucleotide sequence