Experiment: Formate/Acetate-Sulfate (60/10-30 mM) minimal
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpG overlap by 20 nucleotides trpG and trpD overlap by 4 nucleotides trpD and trpC overlap by 8 nucleotides
DVU0465: trpE - anthranilate synthase, component I (TIGR), at 532,853 to 534,451
trpE
DVU0466: trpG - anthranilate synthase, glutamine amidotransferase component (TIGR), at 534,432 to 535,061
trpG
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
Position (kb) 534
535
536 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 533.461 kb on + strand, within trpE at 533.466 kb on + strand, within trpE at 533.489 kb on - strand, within trpE at 533.512 kb on + strand, within trpE at 533.547 kb on - strand, within trpE at 533.554 kb on - strand, within trpE at 533.608 kb on - strand, within trpE at 533.613 kb on - strand, within trpE at 533.615 kb on + strand, within trpE at 533.615 kb on + strand, within trpE at 533.623 kb on - strand, within trpE at 533.651 kb on - strand, within trpE at 533.687 kb on - strand, within trpE at 533.715 kb on - strand, within trpE at 533.719 kb on + strand, within trpE at 533.741 kb on - strand, within trpE at 533.743 kb on + strand, within trpE at 533.751 kb on - strand, within trpE at 533.769 kb on - strand, within trpE at 533.774 kb on + strand, within trpE at 533.871 kb on - strand, within trpE at 534.030 kb on - strand, within trpE at 534.037 kb on + strand, within trpE at 534.073 kb on + strand, within trpE at 534.090 kb on + strand, within trpE at 534.098 kb on - strand, within trpE at 534.107 kb on + strand, within trpE at 534.149 kb on + strand, within trpE at 534.180 kb on - strand, within trpE at 534.256 kb on - strand, within trpE at 534.322 kb on - strand at 534.382 kb on + strand at 534.418 kb on + strand at 534.466 kb on + strand at 534.490 kb on - strand at 534.500 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.563 kb on + strand, within trpG at 534.596 kb on - strand, within trpG at 534.717 kb on - strand, within trpG at 534.766 kb on + strand, within trpG at 534.796 kb on + strand, within trpG at 534.807 kb on + strand, within trpG at 534.895 kb on - strand, within trpG at 534.905 kb on + strand, within trpG at 534.921 kb on - strand, within trpG at 534.935 kb on - strand, within trpG at 535.038 kb on - strand at 535.103 kb on + strand at 535.130 kb on + strand at 535.204 kb on + strand, within trpD at 535.206 kb on - strand, within trpD at 535.229 kb on - strand, within trpD at 535.233 kb on - strand, within trpD at 535.239 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.257 kb on - strand, within trpD at 535.272 kb on + strand, within trpD at 535.447 kb on - strand, within trpD at 535.508 kb on - strand, within trpD at 535.546 kb on + strand, within trpD at 535.591 kb on - strand, within trpD at 535.634 kb on - strand, within trpD at 535.709 kb on + strand, within trpD at 535.756 kb on - strand, within trpD at 535.839 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.849 kb on - strand, within trpD at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfate (60/10-30 mM) minimal remove 533,461 + trpE DVU0465 0.38 -2.0 533,466 + trpE DVU0465 0.38 -1.7 533,489 - trpE DVU0465 0.40 -2.0 533,512 + trpE DVU0465 0.41 -2.4 533,547 - trpE DVU0465 0.43 -0.1 533,554 - trpE DVU0465 0.44 -3.8 533,608 - trpE DVU0465 0.47 -3.0 533,613 - trpE DVU0465 0.48 -1.9 533,615 + trpE DVU0465 0.48 -0.6 533,615 + trpE DVU0465 0.48 -2.4 533,623 - trpE DVU0465 0.48 -1.6 533,651 - trpE DVU0465 0.50 -0.6 533,687 - trpE DVU0465 0.52 -2.0 533,715 - trpE DVU0465 0.54 -0.4 533,719 + trpE DVU0465 0.54 -2.3 533,741 - trpE DVU0465 0.56 -1.7 533,743 + trpE DVU0465 0.56 -3.4 533,751 - trpE DVU0465 0.56 +0.4 533,769 - trpE DVU0465 0.57 -1.8 533,774 + trpE DVU0465 0.58 -1.6 533,871 - trpE DVU0465 0.64 -2.3 534,030 - trpE DVU0465 0.74 -2.1 534,037 + trpE DVU0465 0.74 +0.9 534,073 + trpE DVU0465 0.76 +0.5 534,090 + trpE DVU0465 0.77 -1.6 534,098 - trpE DVU0465 0.78 -1.4 534,107 + trpE DVU0465 0.78 -1.8 534,149 + trpE DVU0465 0.81 -1.9 534,180 - trpE DVU0465 0.83 -1.2 534,256 - trpE DVU0465 0.88 -2.5 534,322 - -2.3 534,382 + -0.3 534,418 + +0.4 534,466 + -1.6 534,490 - -0.1 534,500 - trpG DVU0466 0.11 +0.6 534,501 - trpG DVU0466 0.11 -1.6 534,501 - trpG DVU0466 0.11 -1.7 534,563 + trpG DVU0466 0.21 -1.7 534,596 - trpG DVU0466 0.26 -0.9 534,717 - trpG DVU0466 0.45 +0.9 534,766 + trpG DVU0466 0.53 -1.4 534,796 + trpG DVU0466 0.58 -0.6 534,807 + trpG DVU0466 0.60 -0.7 534,895 - trpG DVU0466 0.73 +0.4 534,905 + trpG DVU0466 0.75 +0.9 534,921 - trpG DVU0466 0.78 -0.7 534,935 - trpG DVU0466 0.80 -2.2 535,038 - +0.5 535,103 + -2.0 535,130 + -1.3 535,204 + trpD DVU0467 0.15 -0.5 535,206 - trpD DVU0467 0.15 -0.5 535,229 - trpD DVU0467 0.17 -2.8 535,233 - trpD DVU0467 0.18 -1.2 535,239 + trpD DVU0467 0.18 -2.4 535,244 + trpD DVU0467 0.19 -2.9 535,244 + trpD DVU0467 0.19 -0.4 535,244 + trpD DVU0467 0.19 -2.1 535,244 + trpD DVU0467 0.19 +0.4 535,244 + trpD DVU0467 0.19 -2.6 535,252 - trpD DVU0467 0.19 -1.6 535,252 - trpD DVU0467 0.19 -0.1 535,252 - trpD DVU0467 0.19 -1.4 535,252 - trpD DVU0467 0.19 +0.9 535,257 - trpD DVU0467 0.20 -2.0 535,272 + trpD DVU0467 0.21 -1.8 535,447 - trpD DVU0467 0.39 -2.6 535,508 - trpD DVU0467 0.45 -2.1 535,546 + trpD DVU0467 0.49 -0.3 535,591 - trpD DVU0467 0.53 -2.4 535,634 - trpD DVU0467 0.58 +0.9 535,709 + trpD DVU0467 0.65 -0.4 535,756 - trpD DVU0467 0.70 +0.9 535,839 + trpD DVU0467 0.78 -2.2 535,841 + trpD DVU0467 0.78 -1.3 535,841 + trpD DVU0467 0.78 +0.9 535,849 - trpD DVU0467 0.79 -0.9 535,871 - trpD DVU0467 0.81 -2.4 535,897 + trpD DVU0467 0.84 -1.2 535,905 - trpD DVU0467 0.85 -0.8 535,920 - trpD DVU0467 0.86 -3.5 535,938 + trpD DVU0467 0.88 -1.4 535,942 - trpD DVU0467 0.88 +1.9 535,942 - trpD DVU0467 0.88 -0.1 535,955 + trpD DVU0467 0.90 -1.1 535,955 + trpD DVU0467 0.90 -1.7 535,963 - -2.2 535,983 + -1.4 535,985 + -2.2 535,985 + +0.9 535,985 + -1.9
Or see this region's nucleotide sequence