Experiment: Formate/Acetate-Sulfate (60/10-30 mM) minimal
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2253 and thyX are separated by 32 nucleotides thyX and ruvB are separated by 17 nucleotides ruvB and ruvA are separated by 0 nucleotides ruvA and DVU2257 are separated by 38 nucleotides DVU2257 and ruvC are separated by 176 nucleotides
DVU2253: DVU2253 - hypothetical protein (TIGR), at 2,349,297 to 2,349,479
DVU2253
DVU2254: thyX - thymidylate synthase ThyX (TIGR), at 2,349,512 to 2,350,249
thyX
DVU2255: ruvB - Holliday junction DNA helicase RuvB (TIGR), at 2,350,267 to 2,351,229
ruvB
DVU2256: ruvA - Holliday junction DNA helicase RuvA (TIGR), at 2,351,230 to 2,351,838
ruvA
DVU2257: DVU2257 - hypothetical protein (TIGR), at 2,351,877 to 2,352,035
DVU2257
DVU2258: ruvC - crossover junction endodeoxyribonuclease RuvC (TIGR), at 2,352,212 to 2,352,712
ruvC
Position (kb)
2350
2351
2352 Strain fitness (log2 ratio)
-1
0
1 at 2349.481 kb on - strand at 2350.259 kb on - strand at 2350.259 kb on - strand at 2350.259 kb on - strand at 2350.259 kb on - strand at 2350.259 kb on - strand at 2350.259 kb on - strand at 2350.297 kb on - strand at 2350.768 kb on - strand, within ruvB at 2350.787 kb on + strand, within ruvB at 2350.795 kb on - strand, within ruvB at 2350.893 kb on - strand, within ruvB at 2350.987 kb on - strand, within ruvB at 2351.245 kb on - strand at 2351.334 kb on - strand, within ruvA at 2351.382 kb on - strand, within ruvA at 2351.878 kb on - strand at 2351.883 kb on - strand at 2351.949 kb on + strand, within DVU2257 at 2351.956 kb on + strand, within DVU2257 at 2352.006 kb on - strand, within DVU2257 at 2352.064 kb on - strand at 2352.203 kb on - strand at 2352.209 kb on - strand at 2352.215 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfate (60/10-30 mM) minimal remove 2,349,481 - +0.5 2,350,259 - +0.2 2,350,259 - -0.4 2,350,259 - +0.0 2,350,259 - -1.3 2,350,259 - -0.2 2,350,259 - -0.3 2,350,297 - +1.0 2,350,768 - ruvB DVU2255 0.52 -0.9 2,350,787 + ruvB DVU2255 0.54 -1.0 2,350,795 - ruvB DVU2255 0.55 -0.0 2,350,893 - ruvB DVU2255 0.65 -0.2 2,350,987 - ruvB DVU2255 0.75 +1.0 2,351,245 - +0.2 2,351,334 - ruvA DVU2256 0.17 -0.3 2,351,382 - ruvA DVU2256 0.25 +0.2 2,351,878 - +0.3 2,351,883 - +1.0 2,351,949 + DVU2257 0.45 -0.7 2,351,956 + DVU2257 0.50 -0.0 2,352,006 - DVU2257 0.81 -0.4 2,352,064 - -0.4 2,352,203 - -0.7 2,352,209 - -0.6 2,352,215 + -0.6
Or see this region's nucleotide sequence