Experiment: Formate/Acetate-Sulfate (60/10-30 mM) minimal
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispE and hslV overlap by 4 nucleotides hslV and DVU1578 are separated by 161 nucleotides DVU1578 and cysS are separated by 5 nucleotides
DVU1576: ispE - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (TIGR), at 1,658,357 to 1,659,217
ispE
DVU1577: hslV - ATP-dependent protease hslV (TIGR), at 1,659,214 to 1,659,759
hslV
DVU1578: DVU1578 - TPR domain protein (TIGR), at 1,659,921 to 1,661,366
DVU1578
DVU1579: cysS - cysteinyl-tRNA synthetase (TIGR), at 1,661,372 to 1,662,829
cysS
Position (kb)
1659
1660
1661
1662 Strain fitness (log2 ratio)
-2
-1
0
1 at 1659.270 kb on - strand, within hslV at 1659.355 kb on + strand, within hslV at 1659.554 kb on - strand, within hslV at 1659.633 kb on - strand, within hslV at 1659.691 kb on + strand, within hslV at 1659.717 kb on + strand at 1659.720 kb on - strand at 1659.729 kb on - strand at 1659.842 kb on + strand at 1659.842 kb on - strand at 1659.847 kb on + strand at 1659.850 kb on - strand at 1659.855 kb on - strand at 1659.860 kb on - strand at 1659.860 kb on - strand at 1659.860 kb on - strand at 1659.860 kb on - strand at 1659.870 kb on - strand at 1659.895 kb on + strand at 1659.903 kb on - strand at 1659.975 kb on - strand at 1659.978 kb on + strand at 1659.978 kb on + strand at 1659.981 kb on - strand at 1660.003 kb on - strand at 1660.005 kb on + strand at 1660.008 kb on - strand at 1660.101 kb on + strand, within DVU1578 at 1660.398 kb on + strand, within DVU1578 at 1660.406 kb on - strand, within DVU1578 at 1660.414 kb on + strand, within DVU1578 at 1660.528 kb on - strand, within DVU1578 at 1660.639 kb on - strand, within DVU1578 at 1660.700 kb on - strand, within DVU1578 at 1660.734 kb on - strand, within DVU1578 at 1660.794 kb on - strand, within DVU1578 at 1660.870 kb on + strand, within DVU1578 at 1660.923 kb on + strand, within DVU1578 at 1661.089 kb on + strand, within DVU1578 at 1661.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfate (60/10-30 mM) minimal remove 1,659,270 - hslV DVU1577 0.10 +1.0 1,659,355 + hslV DVU1577 0.26 -0.0 1,659,554 - hslV DVU1577 0.62 -0.6 1,659,633 - hslV DVU1577 0.77 -0.2 1,659,691 + hslV DVU1577 0.87 -0.9 1,659,717 + -0.0 1,659,720 - -0.0 1,659,729 - -0.0 1,659,842 + -1.3 1,659,842 - -0.3 1,659,847 + -0.1 1,659,850 - -1.0 1,659,855 - -0.2 1,659,860 - +1.0 1,659,860 - -1.6 1,659,860 - +1.0 1,659,860 - -0.3 1,659,870 - +0.6 1,659,895 + -0.8 1,659,903 - -0.5 1,659,975 - -0.4 1,659,978 + -0.0 1,659,978 + -0.0 1,659,981 - -0.6 1,660,003 - +1.0 1,660,005 + -0.7 1,660,008 - -0.8 1,660,101 + DVU1578 0.12 -1.2 1,660,398 + DVU1578 0.33 -0.5 1,660,406 - DVU1578 0.34 -0.6 1,660,414 + DVU1578 0.34 -0.2 1,660,528 - DVU1578 0.42 -0.3 1,660,639 - DVU1578 0.50 -2.5 1,660,700 - DVU1578 0.54 +0.1 1,660,734 - DVU1578 0.56 -0.4 1,660,794 - DVU1578 0.60 -0.2 1,660,870 + DVU1578 0.66 -2.1 1,660,923 + DVU1578 0.69 -1.6 1,661,089 + DVU1578 0.81 +1.0 1,661,250 + -0.4
Or see this region's nucleotide sequence