Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3529 and Dsui_3530 are separated by 198 nucleotides Dsui_3530 and Dsui_3531 overlap by 4 nucleotides Dsui_3531 and Dsui_3532 overlap by 4 nucleotides Dsui_3532 and Dsui_3533 overlap by 1 nucleotides
Dsui_3529: Dsui_3529 - acetolactate synthase, large subunit, biosynthetic type, at 3,795,117 to 3,796,817
_3529
Dsui_3530: Dsui_3530 - DNA-directed RNA polymerase specialized sigma subunit, sigma24, at 3,797,016 to 3,797,573
_3530
Dsui_3531: Dsui_3531 - Protein of unknown function (DUF3619), at 3,797,570 to 3,797,941
_3531
Dsui_3532: Dsui_3532 - Protein of unknown function (DUF3106), at 3,797,938 to 3,798,663
_3532
Dsui_3533: Dsui_3533 - putative membrane protein/domain protein, at 3,798,663 to 3,799,142
_3533
Position (kb)
3797
3798 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3796.878 kb on + strand at 3796.893 kb on - strand at 3796.904 kb on - strand at 3796.907 kb on + strand at 3796.907 kb on + strand at 3796.931 kb on + strand at 3796.951 kb on - strand at 3796.951 kb on - strand at 3796.952 kb on - strand at 3797.002 kb on + strand at 3797.076 kb on - strand, within Dsui_3530 at 3797.078 kb on + strand, within Dsui_3530 at 3797.109 kb on + strand, within Dsui_3530 at 3797.117 kb on - strand, within Dsui_3530 at 3797.118 kb on - strand, within Dsui_3530 at 3797.138 kb on - strand, within Dsui_3530 at 3797.197 kb on - strand, within Dsui_3530 at 3797.314 kb on + strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand at 3797.356 kb on + strand, within Dsui_3530 at 3797.356 kb on + strand, within Dsui_3530 at 3797.364 kb on - strand at 3797.374 kb on - strand, within Dsui_3530 at 3797.380 kb on - strand at 3797.421 kb on - strand at 3797.438 kb on - strand, within Dsui_3530 at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.560 kb on + strand at 3797.567 kb on - strand at 3797.761 kb on + strand, within Dsui_3531 at 3797.775 kb on + strand, within Dsui_3531 at 3797.779 kb on + strand, within Dsui_3531 at 3797.787 kb on - strand, within Dsui_3531 at 3797.916 kb on + strand at 3798.249 kb on + strand, within Dsui_3532 at 3798.366 kb on + strand, within Dsui_3532 at 3798.374 kb on - strand, within Dsui_3532 at 3798.478 kb on - strand, within Dsui_3532 at 3798.532 kb on + strand, within Dsui_3532 at 3798.569 kb on - strand, within Dsui_3532 at 3798.684 kb on + strand at 3798.715 kb on + strand, within Dsui_3533 at 3798.750 kb on + strand, within Dsui_3533 at 3798.856 kb on + strand, within Dsui_3533 at 3798.927 kb on + strand, within Dsui_3533 at 3798.928 kb on + strand, within Dsui_3533
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,796,878 + +0.1 3,796,893 - +0.7 3,796,904 - -2.5 3,796,907 + +0.3 3,796,907 + +0.1 3,796,931 + +0.2 3,796,951 - +0.7 3,796,951 - -0.9 3,796,952 - -2.3 3,797,002 + +0.3 3,797,076 - Dsui_3530 0.11 -0.3 3,797,078 + Dsui_3530 0.11 +1.0 3,797,109 + Dsui_3530 0.17 +0.8 3,797,117 - Dsui_3530 0.18 +0.7 3,797,118 - Dsui_3530 0.18 -0.3 3,797,138 - Dsui_3530 0.22 +0.7 3,797,197 - Dsui_3530 0.32 -0.9 3,797,314 + Dsui_3530 0.53 +1.0 3,797,322 - Dsui_3530 0.55 +0.7 3,797,322 - Dsui_3530 0.55 +0.7 3,797,322 - -0.3 3,797,356 + Dsui_3530 0.61 +1.6 3,797,356 + Dsui_3530 0.61 +0.6 3,797,364 - +0.7 3,797,374 - Dsui_3530 0.64 -0.9 3,797,380 - +0.7 3,797,421 - +0.7 3,797,438 - Dsui_3530 0.76 -0.9 3,797,527 + +1.3 3,797,527 + +1.5 3,797,527 + +0.5 3,797,527 + +0.7 3,797,560 + -0.1 3,797,567 - +0.7 3,797,761 + Dsui_3531 0.51 -3.3 3,797,775 + Dsui_3531 0.55 -1.8 3,797,779 + Dsui_3531 0.56 -1.6 3,797,787 - Dsui_3531 0.58 -0.3 3,797,916 + -2.0 3,798,249 + Dsui_3532 0.43 -1.3 3,798,366 + Dsui_3532 0.59 -2.0 3,798,374 - Dsui_3532 0.60 +0.7 3,798,478 - Dsui_3532 0.74 -1.3 3,798,532 + Dsui_3532 0.82 -0.2 3,798,569 - Dsui_3532 0.87 -1.3 3,798,684 + +0.5 3,798,715 + Dsui_3533 0.11 +1.1 3,798,750 + Dsui_3533 0.18 +0.9 3,798,856 + Dsui_3533 0.40 +0.7 3,798,927 + Dsui_3533 0.55 +1.0 3,798,928 + Dsui_3533 0.55 +0.8
Or see this region's nucleotide sequence