Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3326 and Dsui_3327 are separated by 123 nucleotides Dsui_3327 and Dsui_3328 are separated by 102 nucleotides Dsui_3328 and Dsui_3329 are separated by 242 nucleotides
Dsui_3326: Dsui_3326 - protein-export membrane protein, SecD/SecF family, at 3,566,461 to 3,567,408
_3326
Dsui_3327: Dsui_3327 - cytochrome b, at 3,567,532 to 3,568,248
_3327
Dsui_3328: Dsui_3328 - cytochrome c556, at 3,568,351 to 3,568,821
_3328
Dsui_3329: Dsui_3329 - hypothetical protein, at 3,569,064 to 3,569,381
_3329
Position (kb)
3567
3568
3569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3567.445 kb on + strand at 3567.596 kb on + strand at 3567.659 kb on + strand, within Dsui_3327 at 3567.741 kb on + strand, within Dsui_3327 at 3567.860 kb on - strand, within Dsui_3327 at 3567.962 kb on - strand, within Dsui_3327 at 3568.049 kb on + strand, within Dsui_3327 at 3568.117 kb on - strand at 3568.252 kb on - strand at 3568.305 kb on + strand at 3568.349 kb on + strand at 3568.369 kb on - strand at 3568.424 kb on + strand, within Dsui_3328 at 3568.526 kb on + strand, within Dsui_3328 at 3568.568 kb on - strand, within Dsui_3328 at 3568.568 kb on - strand, within Dsui_3328 at 3568.590 kb on + strand, within Dsui_3328 at 3568.653 kb on - strand, within Dsui_3328 at 3568.663 kb on - strand, within Dsui_3328 at 3568.686 kb on + strand, within Dsui_3328 at 3568.748 kb on - strand, within Dsui_3328 at 3568.780 kb on - strand at 3568.804 kb on - strand at 3568.804 kb on - strand at 3568.810 kb on - strand at 3568.861 kb on + strand at 3568.905 kb on + strand at 3569.079 kb on - strand at 3569.095 kb on + strand at 3569.174 kb on + strand, within Dsui_3329 at 3569.230 kb on + strand, within Dsui_3329
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,567,445 + +0.2 3,567,596 + +2.5 3,567,659 + Dsui_3327 0.18 +3.1 3,567,741 + Dsui_3327 0.29 +2.1 3,567,860 - Dsui_3327 0.46 +0.7 3,567,962 - Dsui_3327 0.60 +0.7 3,568,049 + Dsui_3327 0.72 +2.4 3,568,117 - +0.7 3,568,252 - -0.3 3,568,305 + -3.0 3,568,349 + -2.6 3,568,369 - -0.2 3,568,424 + Dsui_3328 0.15 -0.3 3,568,526 + Dsui_3328 0.37 -0.9 3,568,568 - Dsui_3328 0.46 -0.8 3,568,568 - Dsui_3328 0.46 -0.3 3,568,590 + Dsui_3328 0.51 -0.3 3,568,653 - Dsui_3328 0.64 +0.4 3,568,663 - Dsui_3328 0.66 -0.3 3,568,686 + Dsui_3328 0.71 -0.3 3,568,748 - Dsui_3328 0.84 -0.2 3,568,780 - +1.2 3,568,804 - -0.0 3,568,804 - -0.4 3,568,810 - -0.3 3,568,861 + -1.9 3,568,905 + -1.3 3,569,079 - +0.5 3,569,095 + -2.1 3,569,174 + Dsui_3329 0.35 +0.7 3,569,230 + Dsui_3329 0.52 -0.9
Or see this region's nucleotide sequence