Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3239 and Dsui_3240 are separated by 27 nucleotides Dsui_3240 and Dsui_3241 are separated by 168 nucleotides Dsui_3241 and Dsui_3242 overlap by 17 nucleotides
Dsui_3239: Dsui_3239 - acetyl-CoA acetyltransferase, at 3,482,891 to 3,484,069
_3239
Dsui_3240: Dsui_3240 - cobalamin biosynthesis protein cobD/CbiB, at 3,484,097 to 3,485,017
_3240
Dsui_3241: Dsui_3241 - NTP pyrophosphohydrolase, at 3,485,186 to 3,485,812
_3241
Dsui_3242: Dsui_3242 - hypothetical protein, at 3,485,796 to 3,486,056
_3242
Position (kb)
3484
3485
3486 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3483.104 kb on - strand, within Dsui_3239 at 3483.126 kb on + strand, within Dsui_3239 at 3483.159 kb on - strand, within Dsui_3239 at 3483.170 kb on - strand, within Dsui_3239 at 3483.242 kb on - strand, within Dsui_3239 at 3483.515 kb on + strand, within Dsui_3239 at 3483.539 kb on + strand, within Dsui_3239 at 3483.549 kb on + strand, within Dsui_3239 at 3483.559 kb on + strand, within Dsui_3239 at 3483.559 kb on + strand, within Dsui_3239 at 3483.623 kb on - strand, within Dsui_3239 at 3483.661 kb on - strand, within Dsui_3239 at 3483.680 kb on - strand, within Dsui_3239 at 3483.783 kb on - strand, within Dsui_3239 at 3483.865 kb on - strand, within Dsui_3239 at 3483.920 kb on + strand, within Dsui_3239 at 3483.935 kb on - strand, within Dsui_3239 at 3484.100 kb on + strand at 3484.245 kb on + strand, within Dsui_3240 at 3484.298 kb on - strand, within Dsui_3240 at 3484.389 kb on - strand, within Dsui_3240 at 3484.460 kb on + strand, within Dsui_3240 at 3484.520 kb on - strand, within Dsui_3240 at 3484.525 kb on - strand, within Dsui_3240 at 3484.525 kb on - strand, within Dsui_3240 at 3484.760 kb on - strand, within Dsui_3240 at 3484.809 kb on + strand, within Dsui_3240 at 3484.942 kb on - strand at 3484.976 kb on - strand at 3484.976 kb on - strand at 3485.118 kb on + strand at 3485.168 kb on - strand at 3485.206 kb on + strand at 3485.276 kb on - strand, within Dsui_3241 at 3485.276 kb on - strand, within Dsui_3241 at 3485.276 kb on - strand, within Dsui_3241 at 3485.276 kb on - strand, within Dsui_3241 at 3485.351 kb on + strand, within Dsui_3241 at 3485.359 kb on - strand, within Dsui_3241 at 3485.397 kb on - strand, within Dsui_3241 at 3485.401 kb on + strand, within Dsui_3241 at 3485.428 kb on + strand, within Dsui_3241 at 3485.502 kb on - strand, within Dsui_3241 at 3485.578 kb on + strand, within Dsui_3241 at 3485.611 kb on + strand at 3485.619 kb on - strand, within Dsui_3241 at 3485.619 kb on - strand, within Dsui_3241 at 3485.619 kb on - strand, within Dsui_3241 at 3485.643 kb on + strand, within Dsui_3241 at 3485.776 kb on + strand at 3485.840 kb on + strand, within Dsui_3242 at 3485.998 kb on + strand, within Dsui_3242 at 3485.998 kb on + strand, within Dsui_3242 at 3485.998 kb on + strand, within Dsui_3242 at 3485.998 kb on + strand, within Dsui_3242 at 3486.006 kb on - strand, within Dsui_3242 at 3486.006 kb on - strand, within Dsui_3242 at 3486.008 kb on + strand, within Dsui_3242
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,483,104 - Dsui_3239 0.18 -0.3 3,483,126 + Dsui_3239 0.20 -1.3 3,483,159 - Dsui_3239 0.23 -0.5 3,483,170 - Dsui_3239 0.24 -0.7 3,483,242 - Dsui_3239 0.30 +0.2 3,483,515 + Dsui_3239 0.53 -1.3 3,483,539 + Dsui_3239 0.55 -0.3 3,483,549 + Dsui_3239 0.56 +0.7 3,483,559 + Dsui_3239 0.57 +0.7 3,483,559 + Dsui_3239 0.57 +0.7 3,483,623 - Dsui_3239 0.62 -0.7 3,483,661 - Dsui_3239 0.65 -0.5 3,483,680 - Dsui_3239 0.67 -0.6 3,483,783 - Dsui_3239 0.76 -0.5 3,483,865 - Dsui_3239 0.83 +0.0 3,483,920 + Dsui_3239 0.87 +0.7 3,483,935 - Dsui_3239 0.89 -1.2 3,484,100 + +0.7 3,484,245 + Dsui_3240 0.16 +0.7 3,484,298 - Dsui_3240 0.22 -2.5 3,484,389 - Dsui_3240 0.32 -3.5 3,484,460 + Dsui_3240 0.39 +0.7 3,484,520 - Dsui_3240 0.46 -2.6 3,484,525 - Dsui_3240 0.46 -4.5 3,484,525 - Dsui_3240 0.46 -2.3 3,484,760 - Dsui_3240 0.72 -4.0 3,484,809 + Dsui_3240 0.77 +0.7 3,484,942 - -2.6 3,484,976 - -2.5 3,484,976 - -3.0 3,485,118 + -0.3 3,485,168 - -0.1 3,485,206 + +0.7 3,485,276 - Dsui_3241 0.14 -0.4 3,485,276 - Dsui_3241 0.14 -0.3 3,485,276 - Dsui_3241 0.14 -0.2 3,485,276 - Dsui_3241 0.14 -0.5 3,485,351 + Dsui_3241 0.26 +0.7 3,485,359 - Dsui_3241 0.28 +0.5 3,485,397 - Dsui_3241 0.34 -0.2 3,485,401 + Dsui_3241 0.34 +0.7 3,485,428 + Dsui_3241 0.39 -1.5 3,485,502 - Dsui_3241 0.50 +0.3 3,485,578 + Dsui_3241 0.63 +0.7 3,485,611 + +0.7 3,485,619 - Dsui_3241 0.69 -3.2 3,485,619 - Dsui_3241 0.69 -0.3 3,485,619 - Dsui_3241 0.69 +0.1 3,485,643 + Dsui_3241 0.73 -2.9 3,485,776 + -0.3 3,485,840 + Dsui_3242 0.17 -0.3 3,485,998 + Dsui_3242 0.77 -0.3 3,485,998 + Dsui_3242 0.77 -0.3 3,485,998 + Dsui_3242 0.77 -1.6 3,485,998 + Dsui_3242 0.77 +0.7 3,486,006 - Dsui_3242 0.80 -0.0 3,486,006 - Dsui_3242 0.80 -0.4 3,486,008 + Dsui_3242 0.81 -0.9
Or see this region's nucleotide sequence