Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3196 and Dsui_3197 are separated by 215 nucleotides Dsui_3197 and Dsui_3198 are separated by 93 nucleotides
Dsui_3196: Dsui_3196 - FimV N-terminal domain protein, at 3,429,809 to 3,432,817
_3196
Dsui_3197: Dsui_3197 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 3,433,033 to 3,434,166
_3197
Dsui_3198: Dsui_3198 - 3-isopropylmalate dehydrogenase, at 3,434,260 to 3,435,324
_3198
Position (kb)
3433
3434
3435 Strain fitness (log2 ratio)
-2
-1
0
1 at 3432.041 kb on - strand, within Dsui_3196 at 3432.119 kb on + strand, within Dsui_3196 at 3432.138 kb on + strand, within Dsui_3196 at 3432.143 kb on + strand, within Dsui_3196 at 3432.171 kb on - strand, within Dsui_3196 at 3432.177 kb on + strand at 3432.441 kb on + strand, within Dsui_3196 at 3432.442 kb on - strand, within Dsui_3196 at 3432.525 kb on + strand at 3432.538 kb on - strand at 3432.538 kb on - strand at 3432.574 kb on + strand at 3432.608 kb on + strand at 3432.657 kb on + strand at 3432.670 kb on - strand at 3432.766 kb on - strand at 3432.792 kb on - strand at 3432.831 kb on + strand at 3432.834 kb on + strand at 3432.885 kb on + strand at 3432.885 kb on + strand at 3432.885 kb on + strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3434.206 kb on - strand at 3434.222 kb on + strand at 3434.223 kb on + strand at 3434.231 kb on - strand at 3434.258 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,432,041 - Dsui_3196 0.74 +1.4 3,432,119 + Dsui_3196 0.77 -0.3 3,432,138 + Dsui_3196 0.77 -0.9 3,432,143 + Dsui_3196 0.78 +0.7 3,432,171 - Dsui_3196 0.78 +1.5 3,432,177 + +0.7 3,432,441 + Dsui_3196 0.87 -0.9 3,432,442 - Dsui_3196 0.88 +1.5 3,432,525 + -0.9 3,432,538 - +1.4 3,432,538 - +1.5 3,432,574 + -1.3 3,432,608 + +0.7 3,432,657 + -2.6 3,432,670 - +1.4 3,432,766 - +1.5 3,432,792 - +1.7 3,432,831 + -1.6 3,432,834 + -0.3 3,432,885 + +0.7 3,432,885 + -1.6 3,432,885 + -1.3 3,432,893 - -0.3 3,432,893 - -0.1 3,432,893 - +0.8 3,432,893 - +0.6 3,432,893 - -0.7 3,432,893 - -0.1 3,432,893 - +0.5 3,432,893 - +0.6 3,434,206 - +0.0 3,434,222 + +0.7 3,434,223 + +0.7 3,434,231 - -0.1 3,434,258 + +0.7
Or see this region's nucleotide sequence