Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3028 and Dsui_3029 overlap by 4 nucleotides Dsui_3029 and Dsui_3030 overlap by 4 nucleotides Dsui_3030 and Dsui_3031 are separated by 289 nucleotides
Dsui_3028: Dsui_3028 - coenzyme F420-reducing hydrogenase, gamma subunit, at 3,239,688 to 3,240,245
_3028
Dsui_3029: Dsui_3029 - NADH:ubiquinone oxidoreductase chain G-like protein, at 3,240,242 to 3,240,955
_3029
Dsui_3030: Dsui_3030 - NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit, at 3,240,952 to 3,242,760
_3030
Dsui_3031: Dsui_3031 - enoyl-(acyl-carrier-protein) reductase (NADH), at 3,243,050 to 3,243,814
_3031
Position (kb)
3240
3241
3242
3243 Strain fitness (log2 ratio)
-2
-1
0
1 at 3240.113 kb on - strand, within Dsui_3028 at 3240.364 kb on - strand, within Dsui_3029 at 3240.414 kb on + strand, within Dsui_3029 at 3240.695 kb on - strand, within Dsui_3029 at 3240.726 kb on + strand at 3240.979 kb on - strand at 3241.046 kb on - strand at 3241.142 kb on - strand, within Dsui_3030 at 3241.583 kb on - strand, within Dsui_3030 at 3241.612 kb on - strand, within Dsui_3030 at 3241.861 kb on + strand at 3241.868 kb on + strand at 3241.879 kb on - strand, within Dsui_3030 at 3241.929 kb on - strand, within Dsui_3030 at 3242.079 kb on + strand, within Dsui_3030 at 3242.087 kb on - strand, within Dsui_3030 at 3242.127 kb on + strand, within Dsui_3030 at 3242.159 kb on - strand, within Dsui_3030 at 3242.218 kb on - strand, within Dsui_3030 at 3242.250 kb on + strand, within Dsui_3030 at 3242.282 kb on - strand, within Dsui_3030 at 3242.468 kb on + strand at 3242.486 kb on - strand, within Dsui_3030 at 3242.609 kb on - strand at 3242.609 kb on - strand at 3242.609 kb on - strand at 3242.609 kb on - strand at 3242.728 kb on - strand at 3242.800 kb on - strand at 3242.800 kb on - strand at 3242.826 kb on - strand at 3242.836 kb on - strand at 3242.993 kb on + strand at 3242.994 kb on + strand at 3243.001 kb on - strand at 3243.009 kb on - strand at 3243.042 kb on - strand at 3243.097 kb on + strand at 3243.103 kb on + strand at 3243.118 kb on - strand at 3243.118 kb on - strand at 3243.118 kb on - strand at 3243.118 kb on - strand at 3243.284 kb on + strand, within Dsui_3031 at 3243.321 kb on + strand, within Dsui_3031 at 3243.359 kb on + strand, within Dsui_3031 at 3243.367 kb on - strand, within Dsui_3031 at 3243.380 kb on + strand, within Dsui_3031 at 3243.441 kb on + strand, within Dsui_3031 at 3243.495 kb on + strand, within Dsui_3031 at 3243.508 kb on - strand at 3243.684 kb on + strand, within Dsui_3031
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,240,113 - Dsui_3028 0.76 +0.8 3,240,364 - Dsui_3029 0.17 +0.8 3,240,414 + Dsui_3029 0.24 -0.8 3,240,695 - Dsui_3029 0.63 -0.2 3,240,726 + -0.2 3,240,979 - -1.2 3,241,046 - -0.2 3,241,142 - Dsui_3030 0.11 -1.6 3,241,583 - Dsui_3030 0.35 -0.2 3,241,612 - Dsui_3030 0.36 +0.8 3,241,861 + +0.8 3,241,868 + -0.2 3,241,879 - Dsui_3030 0.51 +0.8 3,241,929 - Dsui_3030 0.54 +0.8 3,242,079 + Dsui_3030 0.62 -1.8 3,242,087 - Dsui_3030 0.63 +0.8 3,242,127 + Dsui_3030 0.65 -0.2 3,242,159 - Dsui_3030 0.67 -1.2 3,242,218 - Dsui_3030 0.70 -1.2 3,242,250 + Dsui_3030 0.72 -0.8 3,242,282 - Dsui_3030 0.74 +0.8 3,242,468 + +0.8 3,242,486 - Dsui_3030 0.85 -1.8 3,242,609 - -2.6 3,242,609 - -0.2 3,242,609 - +0.8 3,242,609 - -0.2 3,242,728 - -0.8 3,242,800 - -0.6 3,242,800 - +0.8 3,242,826 - -0.2 3,242,836 - +0.8 3,242,993 + -0.0 3,242,994 + +0.1 3,243,001 - +0.8 3,243,009 - -1.8 3,243,042 - -0.8 3,243,097 + -0.2 3,243,103 + -0.3 3,243,118 - -1.8 3,243,118 - -0.2 3,243,118 - +0.8 3,243,118 - +0.8 3,243,284 + Dsui_3031 0.31 -0.4 3,243,321 + Dsui_3031 0.35 +0.6 3,243,359 + Dsui_3031 0.40 -0.2 3,243,367 - Dsui_3031 0.41 -0.2 3,243,380 + Dsui_3031 0.43 -0.3 3,243,441 + Dsui_3031 0.51 -0.4 3,243,495 + Dsui_3031 0.58 -0.3 3,243,508 - +0.8 3,243,684 + Dsui_3031 0.83 +0.3
Or see this region's nucleotide sequence