Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3018 and Dsui_3019 are separated by 9 nucleotides Dsui_3019 and Dsui_3020 are separated by 22 nucleotides Dsui_3020 and Dsui_3021 are separated by 57 nucleotides Dsui_3021 and Dsui_3022 overlap by 4 nucleotides
Dsui_3018: Dsui_3018 - OAH/OAS sulfhydrylase, at 3,231,337 to 3,232,638
_3018
Dsui_3019: Dsui_3019 - putative hydrolase or acyltransferase of alpha/beta superfamily, at 3,232,648 to 3,233,433
_3019
Dsui_3020: Dsui_3020 - Protein of unknown function (DUF3124), at 3,233,456 to 3,233,968
_3020
Dsui_3021: Dsui_3021 - putative permease, DMT superfamily, at 3,234,026 to 3,234,913
_3021
Dsui_3022: Dsui_3022 - hypothetical protein, at 3,234,910 to 3,235,074
_3022
Position (kb)
3233
3234 Strain fitness (log2 ratio)
-2
-1
0
1 at 3232.505 kb on - strand at 3232.515 kb on + strand at 3232.759 kb on + strand, within Dsui_3019 at 3233.019 kb on + strand, within Dsui_3019 at 3233.027 kb on - strand, within Dsui_3019 at 3233.040 kb on + strand, within Dsui_3019 at 3233.048 kb on - strand, within Dsui_3019 at 3233.048 kb on - strand, within Dsui_3019 at 3233.216 kb on + strand, within Dsui_3019 at 3233.239 kb on + strand, within Dsui_3019 at 3233.427 kb on + strand at 3233.435 kb on - strand at 3233.435 kb on - strand at 3233.493 kb on - strand at 3233.596 kb on + strand, within Dsui_3020 at 3233.663 kb on + strand, within Dsui_3020 at 3233.671 kb on - strand, within Dsui_3020 at 3233.793 kb on + strand, within Dsui_3020 at 3233.890 kb on + strand, within Dsui_3020 at 3233.914 kb on + strand, within Dsui_3020 at 3233.914 kb on + strand, within Dsui_3020 at 3234.166 kb on + strand, within Dsui_3021 at 3234.166 kb on + strand at 3234.166 kb on + strand, within Dsui_3021 at 3234.180 kb on - strand, within Dsui_3021 at 3234.217 kb on - strand, within Dsui_3021 at 3234.253 kb on + strand, within Dsui_3021 at 3234.266 kb on + strand, within Dsui_3021 at 3234.371 kb on + strand, within Dsui_3021 at 3234.383 kb on + strand at 3234.421 kb on - strand, within Dsui_3021 at 3234.421 kb on - strand, within Dsui_3021 at 3234.428 kb on + strand, within Dsui_3021 at 3234.454 kb on - strand, within Dsui_3021 at 3234.454 kb on - strand, within Dsui_3021 at 3234.586 kb on - strand at 3234.594 kb on - strand, within Dsui_3021 at 3234.610 kb on - strand, within Dsui_3021 at 3234.710 kb on - strand, within Dsui_3021 at 3234.740 kb on + strand, within Dsui_3021 at 3234.751 kb on + strand, within Dsui_3021 at 3234.772 kb on + strand, within Dsui_3021 at 3234.779 kb on + strand, within Dsui_3021 at 3234.894 kb on + strand at 3234.894 kb on + strand at 3234.902 kb on - strand at 3234.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,232,505 - +0.8 3,232,515 + -0.4 3,232,759 + Dsui_3019 0.14 +0.0 3,233,019 + Dsui_3019 0.47 +0.7 3,233,027 - Dsui_3019 0.48 -1.3 3,233,040 + Dsui_3019 0.50 -0.1 3,233,048 - Dsui_3019 0.51 -1.3 3,233,048 - Dsui_3019 0.51 -0.8 3,233,216 + Dsui_3019 0.72 +0.4 3,233,239 + Dsui_3019 0.75 -0.1 3,233,427 + +0.3 3,233,435 - +0.7 3,233,435 - +0.7 3,233,493 - -1.3 3,233,596 + Dsui_3020 0.27 -0.7 3,233,663 + Dsui_3020 0.40 +1.1 3,233,671 - Dsui_3020 0.42 -0.1 3,233,793 + Dsui_3020 0.66 +0.2 3,233,890 + Dsui_3020 0.85 +1.0 3,233,914 + Dsui_3020 0.89 +1.0 3,233,914 + Dsui_3020 0.89 -0.4 3,234,166 + Dsui_3021 0.16 -0.8 3,234,166 + +0.7 3,234,166 + Dsui_3021 0.16 -0.8 3,234,180 - Dsui_3021 0.17 -1.6 3,234,217 - Dsui_3021 0.22 -1.3 3,234,253 + Dsui_3021 0.26 -1.8 3,234,266 + Dsui_3021 0.27 -0.3 3,234,371 + Dsui_3021 0.39 -0.3 3,234,383 + +0.7 3,234,421 - Dsui_3021 0.44 +0.7 3,234,421 - Dsui_3021 0.44 -1.8 3,234,428 + Dsui_3021 0.45 -1.3 3,234,454 - Dsui_3021 0.48 +0.3 3,234,454 - Dsui_3021 0.48 +0.0 3,234,586 - -0.3 3,234,594 - Dsui_3021 0.64 -0.3 3,234,610 - Dsui_3021 0.66 +1.3 3,234,710 - Dsui_3021 0.77 -1.6 3,234,740 + Dsui_3021 0.80 -1.6 3,234,751 + Dsui_3021 0.82 -0.8 3,234,772 + Dsui_3021 0.84 -0.2 3,234,779 + Dsui_3021 0.85 +0.8 3,234,894 + -2.1 3,234,894 + -0.2 3,234,902 - +0.4 3,234,902 - +0.6
Or see this region's nucleotide sequence