Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3012 and Dsui_3013 are separated by 500 nucleotides Dsui_3013 and Dsui_3014 are separated by 9 nucleotides Dsui_3014 and Dsui_3015 overlap by 4 nucleotides
Dsui_3012: Dsui_3012 - hydrogenase (NiFe) small subunit HydA, at 3,225,772 to 3,226,953
_3012
Dsui_3013: Dsui_3013 - hypothetical protein, at 3,227,454 to 3,229,295
_3013
Dsui_3014: Dsui_3014 - outer membrane protein/peptidoglycan-associated (lipo)protein, at 3,229,305 to 3,230,024
_3014
Dsui_3015: Dsui_3015 - hypothetical protein, at 3,230,021 to 3,230,548
_3015
Position (kb)
3227
3228
3229
3230 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3226.557 kb on + strand at 3226.565 kb on + strand, within Dsui_3012 at 3226.883 kb on + strand at 3227.439 kb on + strand at 3227.451 kb on - strand at 3227.464 kb on - strand at 3227.510 kb on + strand at 3227.580 kb on - strand at 3227.649 kb on - strand, within Dsui_3013 at 3227.696 kb on + strand at 3227.914 kb on - strand, within Dsui_3013 at 3228.014 kb on + strand, within Dsui_3013 at 3228.189 kb on + strand, within Dsui_3013 at 3228.321 kb on + strand, within Dsui_3013 at 3228.353 kb on + strand, within Dsui_3013 at 3228.361 kb on - strand at 3228.507 kb on - strand at 3228.682 kb on - strand, within Dsui_3013 at 3228.789 kb on + strand, within Dsui_3013 at 3228.832 kb on - strand, within Dsui_3013 at 3229.021 kb on + strand, within Dsui_3013 at 3229.094 kb on - strand at 3229.115 kb on + strand at 3229.140 kb on + strand at 3229.181 kb on + strand at 3229.195 kb on - strand at 3229.230 kb on + strand at 3229.270 kb on - strand at 3229.290 kb on + strand at 3229.315 kb on - strand at 3229.351 kb on + strand at 3229.409 kb on - strand, within Dsui_3014 at 3229.452 kb on - strand at 3229.710 kb on + strand, within Dsui_3014 at 3229.802 kb on - strand at 3229.862 kb on + strand at 3229.870 kb on - strand at 3229.969 kb on - strand at 3229.989 kb on + strand at 3229.990 kb on - strand at 3230.050 kb on - strand at 3230.262 kb on - strand at 3230.266 kb on + strand at 3230.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,226,557 + +1.7 3,226,565 + Dsui_3012 0.67 +0.7 3,226,883 + +0.7 3,227,439 + +0.2 3,227,451 - +0.7 3,227,464 - -0.8 3,227,510 + -0.8 3,227,580 - +0.7 3,227,649 - Dsui_3013 0.11 +0.7 3,227,696 + +0.7 3,227,914 - Dsui_3013 0.25 -1.6 3,228,014 + Dsui_3013 0.30 -0.1 3,228,189 + Dsui_3013 0.40 -0.4 3,228,321 + Dsui_3013 0.47 +1.0 3,228,353 + Dsui_3013 0.49 +0.8 3,228,361 - +0.7 3,228,507 - +0.7 3,228,682 - Dsui_3013 0.67 -0.3 3,228,789 + Dsui_3013 0.72 +0.1 3,228,832 - Dsui_3013 0.75 +0.7 3,229,021 + Dsui_3013 0.85 +0.3 3,229,094 - +0.7 3,229,115 + +0.5 3,229,140 + -0.1 3,229,181 + -0.4 3,229,195 - -0.8 3,229,230 + +1.2 3,229,270 - -0.8 3,229,290 + -0.3 3,229,315 - -1.8 3,229,351 + +0.6 3,229,409 - Dsui_3014 0.14 -0.8 3,229,452 - -0.3 3,229,710 + Dsui_3014 0.56 +0.5 3,229,802 - +0.7 3,229,862 + -1.3 3,229,870 - +0.7 3,229,969 - -0.3 3,229,989 + -0.1 3,229,990 - +0.7 3,230,050 - -1.3 3,230,262 - +0.7 3,230,266 + +2.3 3,230,280 - +0.7
Or see this region's nucleotide sequence