Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2960 and Dsui_2961 are separated by 3 nucleotides Dsui_2961 and Dsui_2962 overlap by 11 nucleotides Dsui_2962 and Dsui_2963 overlap by 8 nucleotides
Dsui_2960: Dsui_2960 - hypothetical protein, at 3,168,067 to 3,169,053
_2960
Dsui_2961: Dsui_2961 - precorrin isomerase, at 3,169,057 to 3,169,737
_2961
Dsui_2962: Dsui_2962 - cobalamin biosynthesis protein CbiD, at 3,169,727 to 3,170,923
_2962
Dsui_2963: Dsui_2963 - precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit,precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit, at 3,170,916 to 3,172,208
_2963
Position (kb)
3169
3170
3171 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3168.788 kb on + strand, within Dsui_2960 at 3169.054 kb on + strand at 3169.089 kb on + strand at 3169.097 kb on - strand at 3169.198 kb on + strand, within Dsui_2961 at 3169.278 kb on + strand, within Dsui_2961 at 3169.286 kb on - strand, within Dsui_2961 at 3169.404 kb on + strand, within Dsui_2961 at 3169.560 kb on + strand at 3169.640 kb on - strand at 3169.703 kb on + strand at 3169.716 kb on - strand at 3169.754 kb on + strand at 3169.754 kb on + strand at 3169.886 kb on - strand, within Dsui_2962 at 3169.886 kb on - strand, within Dsui_2962 at 3169.943 kb on - strand, within Dsui_2962 at 3170.159 kb on - strand at 3170.250 kb on + strand, within Dsui_2962 at 3170.250 kb on + strand, within Dsui_2962 at 3170.250 kb on + strand, within Dsui_2962 at 3170.258 kb on - strand at 3170.258 kb on - strand at 3170.258 kb on - strand at 3170.365 kb on + strand, within Dsui_2962 at 3170.429 kb on + strand, within Dsui_2962 at 3170.444 kb on + strand, within Dsui_2962 at 3170.533 kb on + strand, within Dsui_2962 at 3170.578 kb on + strand, within Dsui_2962 at 3170.598 kb on + strand, within Dsui_2962 at 3170.626 kb on - strand at 3170.643 kb on + strand, within Dsui_2962 at 3170.930 kb on + strand at 3170.938 kb on - strand at 3170.938 kb on - strand at 3171.217 kb on - strand at 3171.324 kb on - strand at 3171.566 kb on - strand, within Dsui_2963 at 3171.580 kb on + strand, within Dsui_2963 at 3171.737 kb on + strand, within Dsui_2963 at 3171.806 kb on + strand, within Dsui_2963 at 3171.806 kb on + strand, within Dsui_2963 at 3171.814 kb on - strand, within Dsui_2963
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 3,168,788 + Dsui_2960 0.73 -0.2 3,169,054 + -0.2 3,169,089 + +0.2 3,169,097 - -0.2 3,169,198 + Dsui_2961 0.21 -0.9 3,169,278 + Dsui_2961 0.32 -0.9 3,169,286 - Dsui_2961 0.34 -0.8 3,169,404 + Dsui_2961 0.51 +1.2 3,169,560 + +0.8 3,169,640 - +0.8 3,169,703 + -1.1 3,169,716 - +1.8 3,169,754 + +0.6 3,169,754 + -0.8 3,169,886 - Dsui_2962 0.13 -1.5 3,169,886 - Dsui_2962 0.13 -0.8 3,169,943 - Dsui_2962 0.18 -0.2 3,170,159 - +0.8 3,170,250 + Dsui_2962 0.44 +1.4 3,170,250 + Dsui_2962 0.44 +0.1 3,170,250 + Dsui_2962 0.44 +0.5 3,170,258 - -0.2 3,170,258 - +0.8 3,170,258 - -0.2 3,170,365 + Dsui_2962 0.53 -0.7 3,170,429 + Dsui_2962 0.59 +1.9 3,170,444 + Dsui_2962 0.60 +3.6 3,170,533 + Dsui_2962 0.67 -1.5 3,170,578 + Dsui_2962 0.71 +0.4 3,170,598 + Dsui_2962 0.73 +0.8 3,170,626 - -1.5 3,170,643 + Dsui_2962 0.77 +0.8 3,170,930 + +0.8 3,170,938 - +0.8 3,170,938 - +0.8 3,171,217 - -0.8 3,171,324 - -0.2 3,171,566 - Dsui_2963 0.50 -0.2 3,171,580 + Dsui_2963 0.51 -1.5 3,171,737 + Dsui_2963 0.63 -1.4 3,171,806 + Dsui_2963 0.69 -0.2 3,171,806 + Dsui_2963 0.69 +0.8 3,171,814 - Dsui_2963 0.69 +0.8
Or see this region's nucleotide sequence