Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2758 and Dsui_2759 are separated by 283 nucleotides Dsui_2759 and Dsui_2760 are separated by 349 nucleotides Dsui_2760 and Dsui_2761 are separated by 97 nucleotides
Dsui_2758: Dsui_2758 - putative SAM-dependent methyltransferase, at 2,917,617 to 2,918,633
_2758
Dsui_2759: Dsui_2759 - pseudouridine synthase family protein, at 2,918,917 to 2,920,515
_2759
Dsui_2760: Dsui_2760 - translation initiation factor IF-1, at 2,920,865 to 2,921,125
_2760
Dsui_2761: Dsui_2761 - hypothetical protein, at 2,921,223 to 2,921,387
_2761
Position (kb)
2918
2919
2920
2921 Strain fitness (log2 ratio)
-2
-1
0
1 at 2918.123 kb on + strand, within Dsui_2758 at 2918.238 kb on - strand, within Dsui_2758 at 2918.276 kb on + strand, within Dsui_2758 at 2918.276 kb on + strand, within Dsui_2758 at 2918.325 kb on - strand, within Dsui_2758 at 2918.627 kb on + strand at 2918.671 kb on + strand at 2918.671 kb on + strand at 2918.671 kb on + strand at 2918.706 kb on - strand at 2918.877 kb on + strand at 2918.885 kb on - strand at 2918.914 kb on + strand at 2918.922 kb on - strand at 2919.074 kb on + strand at 2919.231 kb on - strand, within Dsui_2759 at 2919.253 kb on + strand, within Dsui_2759 at 2919.306 kb on - strand at 2919.801 kb on + strand, within Dsui_2759 at 2919.869 kb on + strand, within Dsui_2759 at 2920.235 kb on - strand at 2920.250 kb on - strand at 2920.278 kb on + strand, within Dsui_2759 at 2920.286 kb on - strand at 2920.569 kb on + strand at 2920.606 kb on + strand at 2920.730 kb on + strand at 2921.200 kb on + strand at 2921.208 kb on - strand at 2921.369 kb on + strand, within Dsui_2761 at 2921.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,918,123 + Dsui_2758 0.50 +0.1 2,918,238 - Dsui_2758 0.61 +0.9 2,918,276 + Dsui_2758 0.65 -1.8 2,918,276 + Dsui_2758 0.65 -0.4 2,918,325 - Dsui_2758 0.70 -0.1 2,918,627 + +1.5 2,918,671 + -0.1 2,918,671 + +1.0 2,918,671 + +0.9 2,918,706 - -1.1 2,918,877 + -0.1 2,918,885 - -0.7 2,918,914 + -0.2 2,918,922 - +0.9 2,919,074 + -0.2 2,919,231 - Dsui_2759 0.20 -1.1 2,919,253 + Dsui_2759 0.21 +0.1 2,919,306 - +0.9 2,919,801 + Dsui_2759 0.55 +0.2 2,919,869 + Dsui_2759 0.60 +0.5 2,920,235 - -0.1 2,920,250 - +0.9 2,920,278 + Dsui_2759 0.85 -0.2 2,920,286 - -1.1 2,920,569 + +0.6 2,920,606 + +0.1 2,920,730 + -0.4 2,921,200 + -1.8 2,921,208 - -0.1 2,921,369 + Dsui_2761 0.88 +0.3 2,921,498 + +0.8
Or see this region's nucleotide sequence