Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2723 and Dsui_2724 are separated by 36 nucleotides Dsui_2724 and Dsui_2725 are separated by 191 nucleotides Dsui_2725 and Dsui_2726 overlap by 11 nucleotides Dsui_2726 and Dsui_2727 overlap by 20 nucleotides
Dsui_2723: Dsui_2723 - transcription termination factor NusA, at 2,878,955 to 2,880,427
_2723
Dsui_2724: Dsui_2724 - hypothetical protein, at 2,880,464 to 2,880,925
_2724
Dsui_2725: Dsui_2725 - pseudouridine synthase family protein, at 2,881,117 to 2,882,262
_2725
Dsui_2726: Dsui_2726 - segregation and condensation protein B, at 2,882,252 to 2,882,809
_2726
Dsui_2727: Dsui_2727 - hypothetical protein, at 2,882,790 to 2,883,713
_2727
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2880.457 kb on - strand at 2880.491 kb on - strand at 2880.922 kb on - strand at 2880.922 kb on - strand at 2881.054 kb on + strand at 2881.157 kb on + strand at 2882.283 kb on - strand at 2882.331 kb on - strand, within Dsui_2726 at 2882.495 kb on - strand, within Dsui_2726 at 2882.870 kb on - strand at 2882.900 kb on - strand, within Dsui_2727 at 2882.958 kb on - strand, within Dsui_2727 at 2882.968 kb on - strand, within Dsui_2727 at 2883.073 kb on - strand, within Dsui_2727 at 2883.228 kb on - strand, within Dsui_2727
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,880,457 - +0.1 2,880,491 - +1.9 2,880,922 - +0.2 2,880,922 - +3.0 2,881,054 + -1.7 2,881,157 + -0.9 2,882,283 - -0.2 2,882,331 - Dsui_2726 0.14 +0.1 2,882,495 - Dsui_2726 0.44 -1.6 2,882,870 - -0.4 2,882,900 - Dsui_2727 0.12 +0.8 2,882,958 - Dsui_2727 0.18 +0.2 2,882,968 - Dsui_2727 0.19 -0.3 2,883,073 - Dsui_2727 0.31 -1.5 2,883,228 - Dsui_2727 0.47 +1.3
Or see this region's nucleotide sequence