Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2718 and Dsui_2719 are separated by 186 nucleotides Dsui_2719 and Dsui_2720 are separated by 164 nucleotides Dsui_2720 and Dsui_2721 are separated by 7 nucleotides Dsui_2721 and Dsui_2722 are separated by 6 nucleotides
Dsui_2718: Dsui_2718 - polyribonucleotide nucleotidyltransferase, at 2,872,066 to 2,874,189
_2718
Dsui_2719: Dsui_2719 - ribosomal protein S15, at 2,874,376 to 2,874,645
_2719
Dsui_2720: Dsui_2720 - tRNA pseudouridine 55 synthase, at 2,874,810 to 2,875,712
_2720
Dsui_2721: Dsui_2721 - ribosome-binding factor A, at 2,875,720 to 2,876,133
_2721
Dsui_2722: Dsui_2722 - translation initiation factor IF-2, at 2,876,140 to 2,878,932
_2722
Position (kb)
2874
2875
2876 Strain fitness (log2 ratio)
-2
-1
0
1 at 2874.240 kb on + strand at 2874.305 kb on - strand at 2874.334 kb on - strand at 2874.807 kb on + strand at 2874.814 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.852 kb on - strand at 2874.869 kb on + strand at 2874.947 kb on + strand, within Dsui_2720 at 2874.950 kb on - strand, within Dsui_2720 at 2875.037 kb on - strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.110 kb on + strand, within Dsui_2720 at 2875.137 kb on + strand at 2875.145 kb on - strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720 at 2875.399 kb on + strand, within Dsui_2720 at 2875.483 kb on + strand, within Dsui_2720 at 2875.662 kb on + strand at 2875.731 kb on - strand at 2875.815 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,874,240 + -1.7 2,874,305 - +0.7 2,874,334 - -0.0 2,874,807 + -0.1 2,874,814 - -0.1 2,874,815 - +0.4 2,874,815 - -0.3 2,874,815 - -0.1 2,874,815 - +0.0 2,874,852 - +0.1 2,874,869 + +0.9 2,874,947 + Dsui_2720 0.15 -0.7 2,874,950 - Dsui_2720 0.16 +0.5 2,875,037 - Dsui_2720 0.25 +0.8 2,875,063 + Dsui_2720 0.28 -0.1 2,875,063 + Dsui_2720 0.28 +0.9 2,875,110 + Dsui_2720 0.33 -0.1 2,875,137 + +0.9 2,875,145 - Dsui_2720 0.37 +1.1 2,875,166 + Dsui_2720 0.39 +0.9 2,875,166 + Dsui_2720 0.39 -0.1 2,875,399 + Dsui_2720 0.65 -2.4 2,875,483 + Dsui_2720 0.75 +0.9 2,875,662 + -0.1 2,875,731 - -0.6 2,875,815 + -0.7
Or see this region's nucleotide sequence