Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2714 and Dsui_2715 are separated by 14 nucleotides Dsui_2715 and Dsui_2716 are separated by 36 nucleotides Dsui_2716 and Dsui_2717 are separated by 143 nucleotides Dsui_2717 and Dsui_2718 are separated by 115 nucleotides Dsui_2718 and Dsui_2719 are separated by 186 nucleotides Dsui_2719 and Dsui_2720 are separated by 164 nucleotides
Dsui_2714: Dsui_2714 - tRNA-His, at 2,871,102 to 2,871,177
_2714
Dsui_2715: Dsui_2715 - tRNA-Arg, at 2,871,192 to 2,871,268
_2715
Dsui_2716: Dsui_2716 - tRNA-Pro, at 2,871,305 to 2,871,381
_2716
Dsui_2717: Dsui_2717 - RNA polymerase-binding protein DksA, at 2,871,525 to 2,871,950
_2717
Dsui_2718: Dsui_2718 - polyribonucleotide nucleotidyltransferase, at 2,872,066 to 2,874,189
_2718
Dsui_2719: Dsui_2719 - ribosomal protein S15, at 2,874,376 to 2,874,645
_2719
Dsui_2720: Dsui_2720 - tRNA pseudouridine 55 synthase, at 2,874,810 to 2,875,712
_2720
Position (kb)
2872
2873
2874
2875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2871.295 kb on - strand at 2871.528 kb on + strand at 2871.585 kb on + strand, within Dsui_2717 at 2871.644 kb on + strand, within Dsui_2717 at 2871.669 kb on + strand, within Dsui_2717 at 2871.696 kb on + strand, within Dsui_2717 at 2871.742 kb on + strand, within Dsui_2717 at 2871.870 kb on + strand, within Dsui_2717 at 2872.321 kb on - strand, within Dsui_2718 at 2872.327 kb on - strand, within Dsui_2718 at 2874.240 kb on + strand at 2874.305 kb on - strand at 2874.334 kb on - strand at 2874.807 kb on + strand at 2874.814 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.852 kb on - strand at 2874.869 kb on + strand at 2874.947 kb on + strand, within Dsui_2720 at 2874.950 kb on - strand, within Dsui_2720 at 2875.037 kb on - strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.110 kb on + strand, within Dsui_2720 at 2875.137 kb on + strand at 2875.145 kb on - strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 2,871,295 - +2.7 2,871,528 + -3.3 2,871,585 + Dsui_2717 0.14 -2.3 2,871,644 + Dsui_2717 0.28 -2.8 2,871,669 + Dsui_2717 0.34 -1.9 2,871,696 + Dsui_2717 0.40 -3.0 2,871,742 + Dsui_2717 0.51 -4.1 2,871,870 + Dsui_2717 0.81 -2.5 2,872,321 - Dsui_2718 0.12 +0.7 2,872,327 - Dsui_2718 0.12 +0.3 2,874,240 + -1.7 2,874,305 - +0.7 2,874,334 - -0.0 2,874,807 + -0.1 2,874,814 - -0.1 2,874,815 - +0.4 2,874,815 - -0.3 2,874,815 - -0.1 2,874,815 - +0.0 2,874,852 - +0.1 2,874,869 + +0.9 2,874,947 + Dsui_2720 0.15 -0.7 2,874,950 - Dsui_2720 0.16 +0.5 2,875,037 - Dsui_2720 0.25 +0.8 2,875,063 + Dsui_2720 0.28 -0.1 2,875,063 + Dsui_2720 0.28 +0.9 2,875,110 + Dsui_2720 0.33 -0.1 2,875,137 + +0.9 2,875,145 - Dsui_2720 0.37 +1.1 2,875,166 + Dsui_2720 0.39 +0.9 2,875,166 + Dsui_2720 0.39 -0.1
Or see this region's nucleotide sequence